The Use of Fast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry in Protein Therapeutics Discovery and Development

Overview

Journal Drug Discov Today

Specialty Pharmacology

Date 2018 Dec 25

PMID 30583089

Citations 10

Authors

Jing Li

Guodong Chen

Affiliations

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Abstract

Structural analysis of protein therapeutics is a challenging task owing to complexities associated with large molecular size and 3D structures. Recent advances in fast photochemical oxidation of proteins (FPOP) coupled with mass spectrometry (MS) have provided a means to characterize higher order structure (HOS) of protein therapeutics in solution. In this review, the utility of FPOP-MS in protein therapeutics discovery and development is presented, focusing on the principles and applications of epitope mapping for target-protein-protein-therapeutics interactions and biopharmaceutical HOS comparison studies. Future trends in the application of FPOP-MS in protein therapeutics characterization are also described.

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