Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy

Overview

Journal J Vis Exp

Specialties Biology
General Medicine

Date 2018 Dec 25

PMID 30582603

Citations 10

Authors

Eriko Katsuta

Masanori Oshi

Omar M Rashid

Kazuaki Takabe

Affiliations

Soon will be listed here.

Abstract

In vivo mouse models to assess breast cancer progression are essential for cancer research, including preclinical drug developments. However, the majority of the practical and technical details are commonly omitted in published manuscripts which, therefore, makes it challenging to reproduce the models, particularly when it involves surgical techniques. Bioluminescence technology allows for the evaluation of small amounts of cancer cells even when a tumor is not palpable. Utilizing luciferase-expressing cancer cells, we establish a breast cancer orthotopic inoculation technique with a high tumorigenesis rate. Lung metastasis is assessed utilizing an ex vivo technique. We, then, establish a mastectomy model with a low local recurrence rate to assess the metastatic tumor burden. Herein, we describe, in detail, the surgical techniques of orthotopic implantation and mastectomy for breast cancer with a high tumorigenesis rate and low local recurrence rates, respectively, to improve breast cancer model efficiency.

Citing Articles

Targeting Tyro3, Axl, and MerTK Receptor Tyrosine Kinases Significantly Sensitizes Triple-Negative Breast Cancer to CDK4/6 Inhibition.

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PMID: 38927958 PMC: 11202171. DOI: 10.3390/cancers16122253.

Targeting PIM2 by JP11646 results in significant antitumor effects in solid tumors.

Katsuta E, Gil-Moore M, Moore J, Yousif M, Adjei A, Ding Y Int J Oncol. 2022; 61(4).

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H2AX mRNA expression reflects DNA repair, cell proliferation, metastasis, and worse survival in breast cancer.

Katsuta E, Sawant Dessai A, Ebos J, Yan L, Ouchi T, Takabe K Am J Cancer Res. 2022; 12(2):793-804.

PMID: 35261802 PMC: 8900000.

Conflicting roles of expression by subtypes in breast cancer.

Oshi M, Gandhi S, Tokumaru Y, Yan L, Yamada A, Matsuyama R Am J Cancer Res. 2021; 11(10):5094-5110.

PMID: 34765314 PMC: 8569361.

Degree of Early Estrogen Response Predict Survival after Endocrine Therapy in Primary and Metastatic ER-Positive Breast Cancer.

Oshi M, Tokumaru Y, Angarita F, Yan L, Matsuyama R, Endo I Cancers (Basel). 2020; 12(12).

PMID: 33260779 PMC: 7760577. DOI: 10.3390/cancers12123557.

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