The Use of Two Populations of Hepatocytes with Different Triacylglycerol Contents As a Model to Study the Accumulation of Liver Lipid in the Laying Hen

Overview

Journal Biochem J

Specialty Biochemistry

Date 1988 Oct 1

PMID 3058120

Authors

K E Cross

P F Dodds

Affiliations

Soon will be listed here.

Abstract

(1) A method has been developed to separate hepatocytes, isolated from laying hens, according to their densities, using discontinuous density-gradient centrifugation on Nycodenz. (2) The hepatocytes recovered from the interface of the 5% and 10% Nycodenz layers were rich in triacylglycerol and were termed 'fatty' hepatocytes: 'non-fatty' hepatocytes were obtained from the interface of the 15% and 30% Nycodenz layers and contained less than one-quarter as much triacylglycerol. (3) 'Fatty' hepatocytes incorporated radiolabelled glucose and glycerol into total lipid at more than twice the rate of 'non-fatty' cells: the corresponding increases in the incorporation of radiolabelled choline and valine into phospholipid and protein respectively were smaller and not statistically significant. (4) A higher proportion of glycerol and glucose incorporated into total lipid was found to be phospholipid in the 'non-fatty' hepatocytes. (5) A higher proportion of radiolabelled lipid or protein formed from glycerol or valine respectively was secreted into the medium by the 'non-fatty' hepatocytes. (6) The use of these hepatocytes as a model to study fatty liver syndromes is discussed.

References

Smith M, HUBSCHER G . The biosynthesis of glycerides by mitochondria from rat liver. The requirement for a soluble protein. Biochem J. 1966; 101(2):308-16. PMC: 1270110. DOI: 10.1042/bj1010308. View

Brindley D . Some aspects of the physiological and pharmacological control of the synthesis of triacylglycerols and phospholipids. Int J Obes. 1978; 2(1):7-16. View

HAJRA A . Biosynthesis of acyl dihydroxyacetone phosphate in guinea pig liver mitochondria. J Biol Chem. 1968; 243(12):3458-65. View

OHea E, Leveille G . Lipogenesis in isolated adipose tissue of the domestic chick (Gallus domesticus). Comp Biochem Physiol. 1968; 26(1):111-20. DOI: 10.1016/0010-406x(68)90317-4. View

Pearce J . An investigation of the effects of dietary lipid on hepatic lipogenesis in the growing chick. Comp Biochem Physiol B. 1971; 40(1):215-21. DOI: 10.1016/0305-0491(71)90078-2. View

Wolford J, Polin D . Lipid accumulation and hemorrhage in livers of laying chickens. A study on fatty liver-hemorrhagic syndrome (FLHS). Poult Sci. 1972; 51(5):1707-13. DOI: 10.3382/ps.0511707. View

KUDZMA D, Hegstad P, Stoll R . The chick as a laboratory model for the study of estrogen-induced hyperlipidemia. Metabolism. 1973; 22(3):423-34. DOI: 10.1016/0026-0495(73)90034-6. View

Ingebretsen Jr W, WAGLE S . A rapid method for the isolation of large quantities of rat liver parenchymal cells with high anabolic rates. Biochem Biophys Res Commun. 1972; 47(2):403-10. DOI: 10.1016/0006-291x(72)90728-0. View

Lamb R, FALLON H . An enzymatic explanation for dietary induced alterations in hepatic glycerolipid metabolism. Biochim Biophys Acta. 1974; 348(1):179-88. DOI: 10.1016/0005-2760(74)90104-0. View

10.

Sundler R, Akesson B, Nilsson A . Effect of different fatty acids on glycerolipid synthesis in isolated rat hepatocytes. J Biol Chem. 1974; 249(16):5102-7. View

11.

Sundler R, Akesson B . Regulation of phospholipid biosynthesis in isolated rat hepatocytes. Effect of different substrates. J Biol Chem. 1975; 250(9):3359-67. View

12.

Romsos D, Ruiz S, Leveille G . Influence of dietary fat source on hepatic lipogenesis in the fasted-refed chick (Gallus domesticus). Comp Biochem Physiol B. 1976; 53(1):19-22. DOI: 10.1016/0305-0491(76)90089-4. View

13.

Seglen P . Preparation of isolated rat liver cells. Methods Cell Biol. 1976; 13:29-83. DOI: 10.1016/s0091-679x(08)61797-5. View

14.

Dodds P, Gurr M, Brindley D . The glycerol phosphate, dihydroxyacetone phosphate and monoacylglycerol pathways of glycerolipid synthesis in rat adipose-tissue homogenates. Biochem J. 1976; 160(3):693-700. PMC: 1164287. DOI: 10.1042/bj1600693. View

15.

Sturton R, Brindley D . Factors controlling the activities of phosphatidate phosphohydrolase and phosphatidate cytidylyltransferase. The effects of chlorpromazine, demethylimipramine, cinchocaine, norfenfluramine, mepyramine and magnesium ions. Biochem J. 1977; 162(1):25-32. PMC: 1164565. DOI: 10.1042/bj1620025. View

16.

PEARSON A, Butler E . The oestrogenised chick as an experimental model for fatty liver-haemorrhagic syndrome in the fowl. Res Vet Sci. 1978; 24(1):82-6. View

17.

Groener J, Van Golde L . Utilization of exogenously added and endogenously synthesized fatty acids for glycerolipids synthesis in isolated rat hepatocytes. Biochim Biophys Acta. 1978; 529(1):88-95. DOI: 10.1016/0005-2760(78)90106-6. View

18.

Sturton R, Brindley D . Problems encountered in measuring the activity of phosphatidate phosphohydrolase. Biochem J. 1978; 171(1):263-6. PMC: 1184155. DOI: 10.1042/bj1710263. View

19.

Kondrup J, Bro B, Dich J, Grunnet N, Thieden H . Fractionation and characterization of rat hepatocytes isolated from ethanol-induced fatty liver. Lab Invest. 1980; 43(2):182-90. View

20.

Jennings R, Lawson N, Fears R, Brindley D . Stimulation of the activities of phosphatidate phosphohydrolase and tyrosine aminotransferase in rat hepatocytes by glucocorticoids. FEBS Lett. 1981; 133(1):119-22. DOI: 10.1016/0014-5793(81)80485-1. View