Wound Healing Effect of Essential Oil Extracted from DC (Myrtaceae) Leaves

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2018 Dec 23

PMID 30577426

Citations 24

Authors

Sandra Marcia Mazutti da Silva

Claudio Rodrigues Rezende Costa

Guilherme Martins Gelfuso

Eliete Neves Silva Guerra

Yanna Karla de Medeiros Nobrega

Sueli Maria Gomes

Aline Pic-Taylor

Yris Maria Fonseca-Bazzo

Damaris Silveira

Perola de Oliveira Magalhaes

Affiliations

Soon will be listed here.

Abstract

The use of natural oils in topical pharmaceutical preparations has usually presented safe agents for the improvement of human health. Based on research into the immense potential of wound management and healing, we aimed to validate the use of topical natural products by studying the ability of the essential oil of DC leaves (oEd) to stimulate in vitro skin cell migration. Skin cytotoxicity was evaluated using a fibroblast cell line (L929) by MTT assay. The oil chemical profile was investigated by GC-MS. Moreover, the inhibition of lipopolysaccharide (LPS) induced nitric oxide (NO) production in the macrophage cell line (RAW 264.7) tested. The Chick Chorioallantoic Membrane (CAM) assay was used to evaluate the angiogenic activity and irritating potential of the oil. The oEd induces skin cell migration in a scratch assay at a concentration of 542.2 µg/mL. α-humulene and β-caryophyllene, the major compounds of this oil, as determined by GC-MS, may partly explain the migration effect. The inhibition of nitric oxide by oEd and α-humulene suggested an anti-inflammatory effect. The CAM assay showed that treatment with oEd ≤ 292 µg/mL did not cause skin injury, and that it can promote angiogenesis in vivo. Hence, these results indicate the feasibility of the essential oil of DC leaves to developed dermatological products capable of helping the body to repair damaged tissue.

Citing Articles

Bioinputs from DC. (Myrtaceae): Optimization of Ultrasound-Assisted Extraction and Assessment of Antioxidant, Antimicrobial, and Antibiofilm Activities.

Barbosa F, Silva G, Oliveira V, Kubijan L, Costa L, Melo A Molecules. 2025; 30(5).

PMID: 40076338 PMC: 11901441. DOI: 10.3390/molecules30051115.

Immortelle Essential-Oil-Enriched Hydrogel for Diabetic Wound Repair: Development, Characterization, and In Vivo Efficacy Assessment.

Andjic M, Bradic J, Kocovic A, Simic M, Krstonosic V, capo I Pharmaceutics. 2024; 16(10).

PMID: 39458638 PMC: 11510981. DOI: 10.3390/pharmaceutics16101309.

Chitosan-Oxidized Pullulan Hydrogels Loaded with Essential Clove Oil: Synthesis, Characterization, Antioxidant and Antimicrobial Properties.

Suflet D, Constantin M, Pelin I, Popescu I, Rimbu C, Horhogea C Gels. 2024; 10(4).

PMID: 38667646 PMC: 11049474. DOI: 10.3390/gels10040227.

Amazonian useful plants described in the book "Le Pays des Amazones" (1885) of the Brazilian propagandist Baron de Santa-Anna Nery: a historical and ethnobotanical perspective.

Silva L, Oliveira E, Baratto L J Ethnobiol Ethnomed. 2024; 20(1):26.

PMID: 38409064 PMC: 10897987. DOI: 10.1186/s13002-024-00663-2.

Potential Effects of Essential Oil from (Mart.) Kausel on : In Vivo, In Vitro, and In Silico Approaches.

Holanda V, Brito T, Oliveira J, Cunha R, Silva A, Silva W Microorganisms. 2024; 12(1).

PMID: 38276192 PMC: 10819817. DOI: 10.3390/microorganisms12010207.

References

Souza P, Elias S, Simeoni L, de Paula J, Gomes S, Guerra E . Plants from Brazilian Cerrado with potent tyrosinase inhibitory activity. PLoS One. 2012; 7(11):e48589. PMC: 3500240. DOI: 10.1371/journal.pone.0048589. View

Siani A, Souza M, Henriques M, Ramos M . Anti-inflammatory activity of essential oils from Syzygium cumini and Psidium guajava. Pharm Biol. 2013; 51(7):881-7. DOI: 10.3109/13880209.2013.768675. View

Galheigo M, Silva Prado L, Mundin A, Gomes D, Chang R, Lima A . Antidiarrhoeic effect of Eugenia dysenterica DC (Myrtaceae) leaf essential oil. Nat Prod Res. 2015; 30(10):1182-5. DOI: 10.1080/14786419.2015.1043633. View

Prashar A, Locke I, Evans C . Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Prolif. 2006; 39(4):241-8. PMC: 6496679. DOI: 10.1111/j.1365-2184.2006.00384.x. View

MARTIN P, Nunan R . Cellular and molecular mechanisms of repair in acute and chronic wound healing. Br J Dermatol. 2015; 173(2):370-8. PMC: 4671308. DOI: 10.1111/bjd.13954. View

Costa C, Abreu L, Santos E, Presgrave O, Pierucci A, Rodrigues C . Preparation and Evaluation of Chitosan Submicroparticles Containing Pilocarpine for Glaucoma Therapy. Curr Drug Deliv. 2015; 12(5):491-503. DOI: 10.2174/1567201812666150901113240. View

Perini J, Angeli-Gamba T, Alessandra-Perini J, Ferreira L, Nasciutti L, Machado D . Topical application of Acheflan on rat skin injury accelerates wound healing: a histopathological, immunohistochemical and biochemical study. BMC Complement Altern Med. 2015; 15:203. PMC: 4486146. DOI: 10.1186/s12906-015-0745-x. View

Medeiros R, Passos G, Vitor C, Koepp J, Mazzuco T, Pianowski L . Effect of two active compounds obtained from the essential oil of Cordia verbenacea on the acute inflammatory responses elicited by LPS in the rat paw. Br J Pharmacol. 2007; 151(5):618-27. PMC: 2013990. DOI: 10.1038/sj.bjp.0707270. View

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View

10.

Cascaes M, Guilhon G, Aguiar Andrade E, Zoghbi M, Santos L . Constituents and Pharmacological Activities of Myrcia (Myrtaceae): A Review of an Aromatic and Medicinal Group of Plants. Int J Mol Sci. 2015; 16(10):23881-904. PMC: 4632730. DOI: 10.3390/ijms161023881. View

11.

Thakur R, Jain N, Pathak R, Sandhu S . Practices in wound healing studies of plants. Evid Based Complement Alternat Med. 2011; 2011:438056. PMC: 3118986. DOI: 10.1155/2011/438056. View

12.

Stander S, Schmelz M, Metze D, Luger T, Rukwied R . Distribution of cannabinoid receptor 1 (CB1) and 2 (CB2) on sensory nerve fibers and adnexal structures in human skin. J Dermatol Sci. 2005; 38(3):177-88. DOI: 10.1016/j.jdermsci.2005.01.007. View

13.

Barrientos S, Stojadinovic O, Golinko M, Brem H, Tomic-Canic M . Growth factors and cytokines in wound healing. Wound Repair Regen. 2009; 16(5):585-601. DOI: 10.1111/j.1524-475X.2008.00410.x. View

14.

Choi D, Baek Y, Huh J, Ko J, Woo H, Lee J . Stimulatory effect of Cinnamomum cassia and cinnamic acid on angiogenesis through up-regulation of VEGF and Flk-1/KDR expression. Int Immunopharmacol. 2009; 9(7-8):959-67. DOI: 10.1016/j.intimp.2009.04.001. View

15.

Chaves J, Leal P, Pianowisky L, Calixto J . Pharmacokinetics and tissue distribution of the sesquiterpene alpha-humulene in mice. Planta Med. 2008; 74(14):1678-83. DOI: 10.1055/s-0028-1088307. View

16.

Rojo L, Villano C, Joseph G, Schmidt B, Shulaev V, Shuman J . Wound-healing properties of nut oil from Pouteria lucuma. J Cosmet Dermatol. 2010; 9(3):185-95. PMC: 4097019. DOI: 10.1111/j.1473-2165.2010.00509.x. View

17.

Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I . Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol. 2009; 126(3):463-7. DOI: 10.1016/j.jep.2009.09.014. View

18.

Tang L, Pan W, Zhu G, Liu Z, Lv D, Jiang M . Total flavones of enhances angiogenic ability both and . Oncotarget. 2017; 8(41):69768-69778. PMC: 5642515. DOI: 10.18632/oncotarget.19264. View

19.

Koh T, DiPietro L . Inflammation and wound healing: the role of the macrophage. Expert Rev Mol Med. 2011; 13:e23. PMC: 3596046. DOI: 10.1017/S1462399411001943. View

20.

Luo J, Shin V, Liu E, Ye Y, Wu W, So W . Dexamethasone delays ulcer healing by inhibition of angiogenesis in rat stomachs. Eur J Pharmacol. 2004; 485(1-3):275-81. DOI: 10.1016/j.ejphar.2003.11.038. View