Polysialic Acid is Released by Human Umbilical Vein Endothelial Cells (HUVEC) in Vitro

Overview

Journal Cell Biosci

Publisher Biomed Central

Specialty Biology

Date 2018 Dec 18

PMID 30555678

Citations 8

Authors

Sebastian Strubl

Uwe Schubert

Andrea Kuhnle

Alexander Rebl

Negah Ahmadvand

Silvia Fischer

Klaus T Preissner

Sebastian P Galuska

Affiliations

Soon will be listed here.

Abstract

Background: Sialic acids represent common terminal residues on numerous mammalian glycoconjugates, thereby influencing e.g. lumen formation in developing blood vessels. Interestingly, besides monosialylated also polysialylated glycoconjugates are produced by endothelial cells. Polysialic acid (polySia) is formed in several organs during embryonal and postnatal development influencing, for instance, cell migration processes. Furthermore, the function of cytokines like basic fibroblast growth factor (bFGF) is modulated by polySia.

Results: In this study, we demonstrated that human umbilical vein endothelial cells (HUVEC) also secrete polysialylated glycoconjugates. Furthermore, an interaction between polySia and vascular endothelial growth factor (VEGF) was observed. VEGF modulates like bFGF the migration of HUVEC. Since both growth factors interact with polySia, we examined, if polySia modulates the migration of HUVEC. To this end scratch assays were performed showing that the migration of HUVEC is stimulated, when polySia was degraded.

Conclusions: Since polySia can interact with bFGF as well as VEGF and the degradation of polySia resulted in an increased cell migration capacity in the applied scratch assay, we propose that polySia may trap these growth factors influencing their biological activity. Thus, polySia might also contribute to the fine regulation of physiological processes in endothelial cells.

Citing Articles

Knockout of the polysialyltransferases ST8SiaII and ST8SiaIV leads to a dilatation of rete testis during postnatal development.

Humpfle L, Hachem N, Simon P, Weinhold B, Galuska S, Middendorff R Front Physiol. 2023; 14:1240296.

PMID: 37520830 PMC: 10382229. DOI: 10.3389/fphys.2023.1240296.

Correlation of Serum N-Acetylneuraminic Acid with the Risk of Moyamoya Disease.

Liu C, Ge P, Zeng C, Yu X, Zhai Y, Liu W Brain Sci. 2023; 13(6).

PMID: 37371391 PMC: 10296217. DOI: 10.3390/brainsci13060913.

Polysialylation controls immune function of myeloid cells in murine model of pneumococcal pneumonia.

Shinde P, Kiepas A, Zhang L, Sudhir S, Konstantopoulos K, Stamatos N Cell Rep. 2023; 42(6):112648.

PMID: 37339052 PMC: 10592499. DOI: 10.1016/j.celrep.2023.112648.

Milk Polysialic Acid Levels Rapidly Decrease in Line with the N-Acetylneuraminic Acid Concentrations during Early Lactation in Dairy Cows.

Hinterseher J, Gunther J, Zlatina K, Isernhagen L, Viergutz T, Wirthgen E Biology (Basel). 2023; 12(1).

PMID: 36671698 PMC: 9854834. DOI: 10.3390/biology12010005.

Low molecular weight polysialic acid binds to properdin and reduces the activity of the alternative complement pathway.

Shahraz A, Lin Y, Mbroh J, Winkler J, Liao H, Lackmann M Sci Rep. 2022; 12(1):5818.

PMID: 35388026 PMC: 8987038. DOI: 10.1038/s41598-022-09407-2.

References

Kim R, Yokota H, Kim S . Electrophoresis of proteins and protein-protein complexes in a native agarose gel. Anal Biochem. 2000; 282(1):147-9. DOI: 10.1006/abio.2000.4598. View

Stummeyer K, Dickmanns A, Muhlenhoff M, Gerardy-Schahn R, Ficner R . Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F. Nat Struct Mol Biol. 2004; 12(1):90-6. DOI: 10.1038/nsmb874. View

Antoine M, Wirz W, Tag C, Mavituna M, Emans N, Korff T . Expression pattern of fibroblast growth factors (FGFs), their receptors and antagonists in primary endothelial cells and vascular smooth muscle cells. Growth Factors. 2005; 23(2):87-95. DOI: 10.1080/08977190500096004. View

Schauer R . Sialic acids: fascinating sugars in higher animals and man. Zoology (Jena). 2005; 107(1):49-64. DOI: 10.1016/j.zool.2003.10.002. View

Liang C, Park A, Guan J . In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2007; 2(2):329-33. DOI: 10.1038/nprot.2007.30. View

Kermani P, Hempstead B . Brain-derived neurotrophic factor: a newly described mediator of angiogenesis. Trends Cardiovasc Med. 2007; 17(4):140-3. PMC: 2268985. DOI: 10.1016/j.tcm.2007.03.002. View

Goodwin A . In vitro assays of angiogenesis for assessment of angiogenic and anti-angiogenic agents. Microvasc Res. 2007; 74(2-3):172-83. PMC: 2692317. DOI: 10.1016/j.mvr.2007.05.006. View

Varki A . Sialic acids in human health and disease. Trends Mol Med. 2008; 14(8):351-60. PMC: 2553044. DOI: 10.1016/j.molmed.2008.06.002. View

Kanato Y, Kitajima K, Sato C . Direct binding of polysialic acid to a brain-derived neurotrophic factor depends on the degree of polymerization. Glycobiology. 2008; 18(12):1044-53. DOI: 10.1093/glycob/cwn084. View

10.

Tan O, Fadiel A, Chang A, Demir N, Jeffrey R, Horvath T . Estrogens regulate posttranslational modification of neural cell adhesion molecule during the estrogen-induced gonadotropin surge. Endocrinology. 2009; 150(6):2783-90. DOI: 10.1210/en.2008-0927. View

11.

Schauer R . Sialic acids as regulators of molecular and cellular interactions. Curr Opin Struct Biol. 2009; 19(5):507-14. PMC: 7127376. DOI: 10.1016/j.sbi.2009.06.003. View

12.

Hildebrandt H, Muhlenhoff M, Gerardy-Schahn R . Polysialylation of NCAM. Adv Exp Med Biol. 2009; 663:95-109. DOI: 10.1007/978-1-4419-1170-4_6. View

13.

Park H, Pagan L, Tan O, Fadiel A, Demir N, Huang K . Estradiol regulates expression of polysialated neural cell adhesion molecule by human vascular endothelial cells. Reprod Sci. 2010; 17(12):1090-8. DOI: 10.1177/1933719110379649. View

14.

Lackie P, Zuber C, Roth J . Polysialic acid and N-CAM localisation in embryonic rat kidney: mesenchymal and epithelial elements show different patterns of expression. Development. 1990; 110(3):933-47. DOI: 10.1242/dev.110.3.933. View

15.

Strilic B, Eglinger J, Krieg M, Zeeb M, Axnick J, Babal P . Electrostatic cell-surface repulsion initiates lumen formation in developing blood vessels. Curr Biol. 2010; 20(22):2003-9. DOI: 10.1016/j.cub.2010.09.061. View

16.

Oommen S, Gupta S, Vlahakis N . Vascular endothelial growth factor A (VEGF-A) induces endothelial and cancer cell migration through direct binding to integrin {alpha}9{beta}1: identification of a specific {alpha}9{beta}1 binding site. J Biol Chem. 2010; 286(2):1083-92. PMC: 3020715. DOI: 10.1074/jbc.M110.175158. View

17.

Flaumenhaft R, Moscatelli D, Rifkin D . Heparin and heparan sulfate increase the radius of diffusion and action of basic fibroblast growth factor. J Cell Biol. 1990; 111(4):1651-9. PMC: 2116237. DOI: 10.1083/jcb.111.4.1651. View

18.

Potente M, Gerhardt H, Carmeliet P . Basic and therapeutic aspects of angiogenesis. Cell. 2011; 146(6):873-87. DOI: 10.1016/j.cell.2011.08.039. View

19.

Ono S, Hane M, Kitajima K, Sato C . Novel regulation of fibroblast growth factor 2 (FGF2)-mediated cell growth by polysialic acid. J Biol Chem. 2011; 287(6):3710-22. PMC: 3281739. DOI: 10.1074/jbc.M111.276618. View

20.

Curatola A, Huang K, Naftolin F . Dehydroepiandrosterone (DHEA) inhibition of monocyte binding by vascular endothelium is associated with sialylation of neural cell adhesion molecule. Reprod Sci. 2012; 19(1):86-91. PMC: 3343126. DOI: 10.1177/1933719111414210. View