Expression and Characterization of a New PolyG-Specific Alginate Lyase From Marine Bacterium Sp. Q7

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2018 Dec 18

PMID 30555439

Citations 14

Authors

Min Yang

Yuan Yu

Suxiao Yang

Xiaohui Shi

Haijin Mou

Li Li

Affiliations

Soon will be listed here.

Abstract

Alginate lyases play an important role in preparation of alginate oligosaccharides. Although a large number of alginate lyases have been characterized, reports on directional preparation of alginate oligosaccharides by alginate lyases are still rather less. Here, a gene encoding a new alginate lyase AlyM was cloned from sp. Q7 and expressed in . AlyM exhibited the maximumactivity at pH 7.0 and 55°C and showed special preference to poly-guluronic acid (polyG). Glycine promoted the extracellular secretion of AlyM by 3.6 times. PBS and glycerol significantly improved the thermal stability of AlyM, the enzyme activity remained 75 and 78% after heat-treatment at 45°C for 2 h, respectively. ESI-MS analysis suggested that AlyM mainly produced oligosaccharides with degrees of polymerization (DP) of 2-5. The results of H-NMR showed that guluronic acid (G) occupied the reducing end of the end products, indicating that AlyM preferred to degrade the glycosidic bond at the G-X linkage. HPLC analysis showed that the hydrolysis products with a lower degree of polymerization contained more G. Therefore, AlyM shows good potential to produce alginate oligosaccharides with specific M/G ratio and molecular weights.

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