Association of a Potential Functional Mir-520f Rs75598818 G>A Polymorphism with Breast Cancer

Overview

Journal J Genet

Specialty Genetics

Date 2018 Dec 18

PMID 30555079

Citations 3

Authors

Marzieh Meshkat

Hamzeh Mesrian Tanha

Kamran Ghaedi

Mahboobeh Meshkat

Affiliations

Soon will be listed here.

Abstract

Some of the single-nucleotide polymorphisms in miRNA genes have been studied to date to find their association with the risk of breast cancer (BC). However, no study has been conducted to investigate the association of the rs75598818G>A in BC. In the present study, rs75598818 association with BC in an Iranian population has been investigated, and an analysis was performed to predict the function of rs75598818 polymorphism in BC. The rs75598818 was genotyped in 129 BC patients and 144 healthy women, using the PCR-RFLP method. The frequency of alleles and genotypes were considered to find the associations between rs75598818 alleles/genotypes, and BC risk and pathological characteristics of the patients. Statistical analysis showed that the rs75598818 GA genotype was significantly associated with BC (GA versus GG, OR=0.50, 95% CI: 0.25-0.98, =0.041), highstage BC (stage III/IV versus I/II, GA versus GG, OR=0.27, 95% CI: 0.09-0.81, =0.015), and HER-2 positive status (GA versus GG, OR=19.00, 95% CI: 4.64-77.82, <0.001). Notably, the rs75598818 GA genotype has a negative association pattern since it reduces the risk of BC and high stage BC. Conversely, it increases the risk of HER-2 positivity. Computational results suggested that the rs75598818 polymorphism affects the stability of stem-loop and as a result -3p production that is a potential tumour suppressor. A contribution of the rs75598818 polymorphism to BC had been unexplored before. In the present study, we performed an association study and a bioinformatics approach to evaluate this polymorphism in BC. However, further functional experiments and large-scale association studies with various ethnicities are required to elaborate our findings.

Citing Articles

Association between single-nucleotide polymorphisms in miRNA and breast cancer risk: an updated review.

Arancibia T, Morales-Pison S, Maldonado E, Jara L Biol Res. 2021; 54(1):26.

PMID: 34454612 PMC: 8401249. DOI: 10.1186/s40659-021-00349-z.

Transcription factor AP-4 (TFAP4)-upstream ORF coding 66 aa inhibits the malignant behaviors of glioma cells by suppressing the TFAP4/long noncoding RNA 00520/microRNA-520f-3p feedback loop.

Wang Y, Yang C, Liu X, Zheng J, Zhang F, Wang D Cancer Sci. 2020; 111(3):891-906.

PMID: 31943575 PMC: 7060482. DOI: 10.1111/cas.14308.

Evidences from a Systematic Review and Meta-Analysis Unveil the Role of MiRNA Polymorphisms in the Predisposition to Female Neoplasms.

Bastami M, Choupani J, Saadatian Z, Zununi Vahed S, Ouladsahebmadarek E, Mansoori Y Int J Mol Sci. 2019; 20(20).

PMID: 31615040 PMC: 6834313. DOI: 10.3390/ijms20205088.

References

Hamosh A, Scott A, Amberger J, Bocchini C, McKusick V . Online Mendelian Inheritance in Man (OMIM), a knowledgebase of human genes and genetic disorders. Nucleic Acids Res. 2004; 33(Database issue):D514-7. PMC: 539987. DOI: 10.1093/nar/gki033. View

Bentwich I, Avniel A, Karov Y, Aharonov R, Gilad S, Barad O . Identification of hundreds of conserved and nonconserved human microRNAs. Nat Genet. 2005; 37(7):766-70. DOI: 10.1038/ng1590. View

Zhang B, Pan X, Cobb G, Anderson T . microRNAs as oncogenes and tumor suppressors. Dev Biol. 2006; 302(1):1-12. DOI: 10.1016/j.ydbio.2006.08.028. View

Griffiths-Jones S, Saini H, van Dongen S, Enright A . miRBase: tools for microRNA genomics. Nucleic Acids Res. 2007; 36(Database issue):D154-8. PMC: 2238936. DOI: 10.1093/nar/gkm952. View

Anderson B, Jakesz R . Breast cancer issues in developing countries: an overview of the Breast Health Global Initiative. World J Surg. 2008; 32(12):2578-85. DOI: 10.1007/s00268-007-9454-z. View

Huang D, Sherman B, Lempicki R . Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009; 4(1):44-57. DOI: 10.1038/nprot.2008.211. View

Ryan B, Robles A, Harris C . Genetic variation in microRNA networks: the implications for cancer research. Nat Rev Cancer. 2010; 10(6):389-402. PMC: 2950312. DOI: 10.1038/nrc2867. View

Keklikoglou I, Koerner C, Schmidt C, Zhang J, Heckmann D, Shavinskaya A . MicroRNA-520/373 family functions as a tumor suppressor in estrogen receptor negative breast cancer by targeting NF-κB and TGF-β signaling pathways. Oncogene. 2011; 31(37):4150-63. DOI: 10.1038/onc.2011.571. View

Chen Q, Wang Q, Zhang B . Ethnicity modifies the association between functional microRNA polymorphisms and breast cancer risk: a HuGE meta-analysis. Tumour Biol. 2013; 35(1):529-43. DOI: 10.1007/s13277-013-1074-7. View

10.

Omrani M, Hashemi M, Eskandari-Nasab E, Hasani S, Mashhadi M, Arbabi F . hsa-mir-499 rs3746444 gene polymorphism is associated with susceptibility to breast cancer in an Iranian population. Biomark Med. 2014; 8(2):259-67. DOI: 10.2217/bmm.13.118. View

11.

Marjan M, Hamzeh M, Rahman E, Sadeq V . A computational prospect to aspirin side effects: aspirin and COX-1 interaction analysis based on non-synonymous SNPs. Comput Biol Chem. 2014; 51:57-62. DOI: 10.1016/j.compbiolchem.2014.05.002. View

12.

Ha M, Kim V . Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol. 2014; 15(8):509-24. DOI: 10.1038/nrm3838. View

13.

Harvey H, Piskareva O, Creevey L, Alcock L, Buckley P, OSullivan M . Modulation of chemotherapeutic drug resistance in neuroblastoma SK-N-AS cells by the neural apoptosis inhibitory protein and miR-520f. Int J Cancer. 2014; 136(7):1579-88. DOI: 10.1002/ijc.29144. View

14.

Dweep H, Gretz N . miRWalk2.0: a comprehensive atlas of microRNA-target interactions. Nat Methods. 2015; 12(8):697. DOI: 10.1038/nmeth.3485. View

15.

Srinivasan S, Clements J, Batra J . Single nucleotide polymorphisms in clinics: Fantasy or reality for cancer?. Crit Rev Clin Lab Sci. 2015; 53(1):29-39. DOI: 10.3109/10408363.2015.1075469. View

16.

Chou C, Chang N, Shrestha S, Hsu S, Lin Y, Lee W . miRTarBase 2016: updates to the experimentally validated miRNA-target interactions database. Nucleic Acids Res. 2015; 44(D1):D239-47. PMC: 4702890. DOI: 10.1093/nar/gkv1258. View

17.

Mesrian Tanha H, Naeini M, Rahgozar S, Moafi A, Honardoost M . Integrative computational in-depth analysis of dysregulated miRNA-mRNA interactions in drug-resistant pediatric acute lymphoblastic leukemia cells: an attempt to obtain new potential gene-miRNA pathways involved in response to treatment. Tumour Biol. 2015; 37(6):7861-72. DOI: 10.1007/s13277-015-4553-1. View

18.

Hashemi M, Sanaei S, Mashhadi M, Hashemi S, Taheri M, Ghavami S . Association study of hsa-mir-603 rs11014002 polymorphism and risk of breast cancer in a sample of Iranian population. Cell Mol Biol (Noisy-le-grand). 2016; 61(8):69-73. View

19.

Hashemi M, Sanaei S, Rezaei M, Bahari G, Hashemi S, Mashhadi M . miR-608 rs4919510 C>G polymorphism decreased the risk of breast cancer in an Iranian subpopulation. Exp Oncol. 2016; 38(1):57-9. View

20.

Bagheri F, Mesrian Tanha H, Naeini M, Ghaedi K, Azadeh M . Tumor-promoting function of single nucleotide polymorphism rs1836724 (C3388T) alters multiple potential legitimate microRNA binding sites at the 3'-untranslated region of ErbB4 in breast cancer. Mol Med Rep. 2016; 13(5):4494-8. DOI: 10.3892/mmr.2016.5078. View