Up-Regulation of MiR-1915 Inhibits Proliferation, Invasion, and Migration of -Infected Gastric Cancer Cells Via Targeting RAGE

Overview

Journal Yonsei Med J

Specialty General Medicine

Date 2018 Dec 17

PMID 30554489

Citations 7

Authors

Xin Cai Xu

Wen Bin Zhang

Chun Xing Li

Hua Gao

Qi Pei

Bo Wei Cao

Tie Han He

Affiliations

Soon will be listed here.

Abstract

Purpose: -infected gastric cancer (GC) is known to be a fatal malignant tumor, but the molecular mechanisms underlying its proliferation, invasion, and migration remain far from being completely understood. Our aim in this study was to explore miR-1915 expression and its molecular mechanisms in regulating proliferation, invasion, and migration of -infected GC cells.

Materials And Methods: Quantitative real-time PCR and western blot analysis were performed to determine miR-1915 and receptor for advanced glycation end product (RAGE) expression in -infected GC tissues and gastritis tissues, as well as human gastric mucosal cell line GES-1 and human GC cell lines SGC-7901 and MKN45. CCK8 assay and transwell assay were performed to detect the proliferation, invasion, and migration capabilities. MiR-1915 mimics and miR-1915 inhibitor were transfected into GC cells to determine the target relationship between miR-1915 and RAGE.

Results: MiR-1915 was under-expressed, while RAGE was over-expressed in -infected GC tissues and GC cells. Over-expressed miR-1915 could attenuate cellular proliferation, invasion, and migration capacities. RAGE was confirmed to be the target gene of miR-1915 by bioinformatics analysis and luciferase reporter assay. Moreover, -infected GC cellular proliferation, invasion, and migration were inhibited after treatment with pcDNA-RAGE.

Conclusion: MiR-1915 exerted tumor-suppressive effects on cellular proliferation, invasion, and migration of -infected GC cells via targeting RAGE, which provided an innovative target candidate for treatment of -infected GC.

Citing Articles

Roles of microRNAs and exosomes in Helicobacter pylori associated gastric cancer.

Xu X, Shao S, Guo D, Ge L, Wang Z, Liu P Mol Biol Rep. 2022; 50(1):889-897.

PMID: 36367659 DOI: 10.1007/s11033-022-08073-x.

Host miRNAs-microbiota interactions in gastric cancer.

Yang Y, Huang Y, Lin W, Liu J, Chen X, Chen C J Transl Med. 2022; 20(1):52.

PMID: 35093110 PMC: 8800214. DOI: 10.1186/s12967-022-03264-3.

MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer.

Pan H, Pan Z, Guo F, Meng F, Zu L, Fan Y BMC Cancer. 2021; 21(1):1218.

PMID: 34774019 PMC: 8590782. DOI: 10.1186/s12885-021-08961-8.

Function of Non-coding RNA in -Infected Gastric Cancer.

Wang C, Hu Y, Yang H, Wang S, Zhou B, Bao Y Front Mol Biosci. 2021; 8:649105.

PMID: 34046430 PMC: 8144459. DOI: 10.3389/fmolb.2021.649105.

Aberrant Expression of Long Non-coding RNAs in Exosomes in Follicle Fluid From PCOS Patients.

Wang L, Fan H, Zou Y, Yuan Q, Hu X, Chen X Front Genet. 2021; 11:608178.

PMID: 33679867 PMC: 7925891. DOI: 10.3389/fgene.2020.608178.

References

Hirata Y, Maeda S, Mitsuno Y, Akanuma M, Yamaji Y, Ogura K . Helicobacter pylori activates the cyclin D1 gene through mitogen-activated protein kinase pathway in gastric cancer cells. Infect Immun. 2001; 69(6):3965-71. PMC: 98458. DOI: 10.1128/IAI.69.6.3965-3971.2001. View

Mitsuno Y, Yoshida H, Maeda S, Ogura K, Hirata Y, Kawabe T . Helicobacter pylori induced transactivation of SRE and AP-1 through the ERK signalling pathway in gastric cancer cells. Gut. 2001; 49(1):18-22. PMC: 1728350. DOI: 10.1136/gut.49.1.18. View

Kuniyasu H, Oue N, Wakikawa A, Shigeishi H, Matsutani N, Kuraoka K . Expression of receptors for advanced glycation end-products (RAGE) is closely associated with the invasive and metastatic activity of gastric cancer. J Pathol. 2002; 196(2):163-70. DOI: 10.1002/path.1031. View

Parkin D . The global health burden of infection-associated cancers in the year 2002. Int J Cancer. 2006; 118(12):3030-44. DOI: 10.1002/ijc.21731. View

Talley N, Zinsmeister A, Weaver A, DiMagno E, CARPENTER H, Perez-Perez G . Gastric adenocarcinoma and Helicobacter pylori infection. J Natl Cancer Inst. 1991; 83(23):1734-9. DOI: 10.1093/jnci/83.23.1734. View

Zhang Z, Li Z, Gao C, Chen P, Chen J, Liu W . miR-21 plays a pivotal role in gastric cancer pathogenesis and progression. Lab Invest. 2008; 88(12):1358-66. DOI: 10.1038/labinvest.2008.94. View

Mbulaiteye S, Hisada M, El-Omar E . Helicobacter Pylori associated global gastric cancer burden. Front Biosci (Landmark Ed). 2009; 14(4):1490-504. PMC: 2654592. DOI: 10.2741/3320. View

Saito Y, Suzuki H, Tsugawa H, Nakagawa I, Matsuzaki J, Kanai Y . Chromatin remodeling at Alu repeats by epigenetic treatment activates silenced microRNA-512-5p with downregulation of Mcl-1 in human gastric cancer cells. Oncogene. 2009; 28(30):2738-44. DOI: 10.1038/onc.2009.140. View

Wada R, Akiyama Y, Hashimoto Y, Fukamachi H, Yuasa Y . miR-212 is downregulated and suppresses methyl-CpG-binding protein MeCP2 in human gastric cancer. Int J Cancer. 2009; 127(5):1106-14. DOI: 10.1002/ijc.25126. View

10.

Hashimoto Y, Akiyama Y, Otsubo T, Shimada S, Yuasa Y . Involvement of epigenetically silenced microRNA-181c in gastric carcinogenesis. Carcinogenesis. 2010; 31(5):777-84. DOI: 10.1093/carcin/bgq013. View

11.

Lai K, Koh K, Loh M, Tada K, Subramaniam M, Lim X . MicroRNA-130b regulates the tumour suppressor RUNX3 in gastric cancer. Eur J Cancer. 2010; 46(8):1456-63. DOI: 10.1016/j.ejca.2010.01.036. View

12.

Jemal A, Siegel R, Xu J, Ward E . Cancer statistics, 2010. CA Cancer J Clin. 2010; 60(5):277-300. DOI: 10.3322/caac.20073. View

13.

Zhu L, Liu T, Tang H, Tian R, Su C, Liu M . MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor. FEBS J. 2010; 277(18):3726-34. DOI: 10.1111/j.1742-4658.2010.07773.x. View

14.

Wang J, Yu J, Kang W, Ma Z . Treatment strategy for early gastric cancer. Surg Oncol. 2011; 21(2):119-23. DOI: 10.1016/j.suronc.2010.12.004. View

15.

Xu Y, Zhao F, Wang Z, Song Y, Luo Y, Zhang X . MicroRNA-335 acts as a metastasis suppressor in gastric cancer by targeting Bcl-w and specificity protein 1. Oncogene. 2011; 31(11):1398-407. PMC: 3312408. DOI: 10.1038/onc.2011.340. View

16.

Liang H, Zhong Y, Zhou S, Peng L . Knockdown of RAGE expression inhibits colorectal cancer cell invasion and suppresses angiogenesis in vitro and in vivo. Cancer Lett. 2011; 313(1):91-8. DOI: 10.1016/j.canlet.2011.08.028. View

17.

DiNorcia J, Lee M, Moroziewicz D, Winner M, Suman P, Bao F . RAGE gene deletion inhibits the development and progression of ductal neoplasia and prolongs survival in a murine model of pancreatic cancer. J Gastrointest Surg. 2011; 16(1):104-12. PMC: 4049447. DOI: 10.1007/s11605-011-1754-9. View

18.

Kim C, Kim H, Rettig R, Kim J, Lee E, Aprelikova O . miRNA signature associated with outcome of gastric cancer patients following chemotherapy. BMC Med Genomics. 2011; 4:79. PMC: 3287139. DOI: 10.1186/1755-8794-4-79. View

19.

Xu K, Liang X, Cui D, Wu Y, Shi W, Liu J . miR-1915 inhibits Bcl-2 to modulate multidrug resistance by increasing drug-sensitivity in human colorectal carcinoma cells. Mol Carcinog. 2011; 52(1):70-8. DOI: 10.1002/mc.21832. View

20.

Elangovan I, Thirugnanam S, Chen A, Zheng G, Bosland M, Kajdacsy-Balla A . Targeting receptor for advanced glycation end products (RAGE) expression induces apoptosis and inhibits prostate tumor growth. Biochem Biophys Res Commun. 2011; 417(4):1133-8. DOI: 10.1016/j.bbrc.2011.12.060. View