DEPArray™ System: An Automatic Image-based Sorter for Isolation of Pure Circulating Tumor Cells

Overview

Journal Cytometry A

Specialties Cell Biology
Radiology

Date 2018 Dec 15

PMID 30551261

Citations 41

Authors

Mariano Di Trapani

Nicolo Manaresi

Gianni Medoro

Affiliations

Soon will be listed here.

Abstract

Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single-cell level can inform on the genomic landscape of a tumor. All CTC enrichment methods bear a burden of contaminating normal cells, which mandate a further step of purification to enable reliable downstream genetic analysis. Here, we describe the DEPArray™ technology, a microchip-based digital sorter, which combines precise microfluidic and microelectronic enabling precise, image-based isolation of single CTCs, which can then be analyzed by Next Generation Sequencing (NGS) methods. © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

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