BUB-1 Promotes Amphitelic Chromosome Biorientation Via Multiple Activities at the Kinetochore

Overview

Journal Elife

Specialty Biology

Date 2018 Dec 15

PMID 30547880

Citations 17

Authors

Frances Edwards

Gilliane Maton

Nelly Gareil

Julie C Canman

Julien Dumont

Affiliations

Soon will be listed here.

Abstract

Accurate chromosome segregation relies on bioriented amphitelic attachments of chromosomes to microtubules of the mitotic spindle, in which sister chromatids are connected to opposite spindle poles. BUB-1 is a protein of the Spindle Assembly Checkpoint (SAC) that coordinates chromosome attachment with anaphase onset. BUB-1 is also required for accurate sister chromatid segregation independently of its SAC function, but the underlying mechanism remains unclear. Here we show that, in embryos, BUB-1 accelerates the establishment of non-merotelic end-on kinetochore-microtubule attachments by recruiting the RZZ complex and its downstream partner dynein-dynactin at the kinetochore. In parallel, BUB-1 limits attachment maturation by the SKA complex. This activity opposes kinetochore-microtubule attachment stabilisation promoted by CLS-2-dependent kinetochore-microtubule assembly. BUB-1 is therefore a SAC component that coordinates the function of multiple downstream kinetochore-associated proteins to ensure accurate chromosome segregation.

Citing Articles

4D Microscopy and Tracking of Chromosomes and the Spindle in C. elegans Early Embryos.

Dumont J, Maton G Methods Mol Biol. 2024; 2872:141-156.

PMID: 39616574 DOI: 10.1007/978-1-0716-4224-5_10.

Unveiling inter-embryo variability in spindle length over time: Towards quantitative phenotype analysis.

Le Cunff Y, Chesneau L, Pastezeur S, Pinson X, Soler N, Fairbrass D PLoS Comput Biol. 2024; 20(9):e1012330.

PMID: 39236069 PMC: 11376571. DOI: 10.1371/journal.pcbi.1012330.

Phospho-KNL-1 recognition by a TPR domain targets the BUB-1-BUB-3 complex to C. elegans kinetochores.

Houston J, Vissotsky C, Deep A, Hakozaki H, Crews E, Oegema K J Cell Biol. 2024; 223(7).

PMID: 38578284 PMC: 10996584. DOI: 10.1083/jcb.202402036.

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