MiR-21-5p Induces Cell Proliferation by Targeting TGFBI in Non-small Cell Lung Cancer Cells

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2018 Dec 14

PMID 30542417

Citations 21

Authors

Liang Yan

Jinzhu Ma

Yi Wang

Jiawei Zan

Zhen Wang

Yu Zhu

Yiping Zhu

Liefeng Ling

Long Cao

Xin Liu

Shu Li

Lei Xu

Zhilin Qi

Liuwang Nie

Yao Zhang

Affiliations

Soon will be listed here.

Abstract

The mortality rate of non-small cell lung cancer (NSCLC) remains high worldwide. miR-21-5p plays an important part in many cancer types, including NSCLC. However, the effect of miR-21-5p in NSCLC tumorigenesis remains poorly understood. The present study investigated whether miR-21-5p promoted NSCLC cell proliferation . In order to study the molecular mechanism by which miR-21-5p contributes to NSCLC progression, three bioinformatics algorithms were used to predict the genes which miR-21-5p targeted. TGFBI was identfieid as a putative direct target in NSCLC cells via the luciferase reporter assay. Furthermore, miR-21-5p downregulated TGFBI protein expression by a post-transcriptional mechanism via western blotting and a reverse transcription-quantitative polymerase chain reaction analysis. Finally, TGFBI exhibited opposing effects to those of miR-21-5p on NSCLC cells, suggesting that miR-21-5p may promote cell proliferation by negative regulation of TGFBI. These results suggest miR-21-5p promote the proliferation of NSCLC cells via inhibiting TGFBI expression.

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