Influence of Nanofiber Orientation on Morphological and Mechanical Properties of Electrospun Chitosan Mats

Overview

Journal J Healthc Eng

Specialty Biomedical Engineering

Date 2018 Dec 13

PMID 30538809

Citations 15

Authors

Paola Nitti

Nunzia Gallo

Lara Natta

Francesca Scalera

Barbara Palazzo

Alessandro Sannino

Francesca Gervaso

Affiliations

Soon will be listed here.

Abstract

This work explored the use of chitosan (Cs) and poly(ethylene oxide) (PEO) blends for the fabrication of electrospun fiber-orientated meshes potentially suitable for engineering fiber-reinforced soft tissues such as tendons, ligaments, or meniscus. To mimic the fiber alignment present in native tissue, the CS/PEO blend solution was electrospun using a traditional static plate, a rotating drum collector, and a rotating disk collector to get, respectively, random, parallel, circumferential-oriented fibers. The effects of the different orientations (parallel or circumferential) and high-speed rotating collector influenced fiber morphology, leading to a reduction in nanofiber diameters and an improvement in mechanical properties.

Citing Articles

Electro-spun piezoelectric PLLA smart composites as a scaffold on bone fracture: A review.

Mirghaffari M, Mahmoodiyan A, Mahboubizadeh S, Shahbazi A, Soleimani Y, Mirghaffari S Regen Ther. 2025; 28:591-605.

PMID: 40061293 PMC: 11889349. DOI: 10.1016/j.reth.2025.01.026.

Fabricating ultra-thin nanofiber structures towards the advanced MEA of fuel cells: investigation of the degree of alignment, diameter, bead generation, and precision with Taguchi designs.

Yusro M, Hacker V Nanoscale Adv. 2024; .

PMID: 39359351 PMC: 11441462. DOI: 10.1039/d4na00558a.

Analysis and Application of Poly(vinyl alcohol) (PVA) and Polyacrylonitrile (PAN) Nanofiber Membrane-Based Triboelectric Nanogenerators.

Pragalathan S, Venugopal V ACS Omega. 2024; 9(36):37802-37813.

PMID: 39281960 PMC: 11391443. DOI: 10.1021/acsomega.4c03625.

Estimation of Digital Porosity of Electrospun Veils by Image Analysis.

Cuahuizo-Huitzil G, Olivares-Xometl O, Arellanes-Lozada P, Laguna Cortes J, Arriola Morales J, Santacruz-Vazquez C Polymers (Basel). 2024; 16(2).

PMID: 38276707 PMC: 10820155. DOI: 10.3390/polym16020300.

Electrospun Poly(carbonate-urea-urethane)s Nonwovens with Shape-Memory Properties as a Potential Biomaterial.

Rolinska K, Bakhshi H, Balk M, Blocki A, Panwar A, Puchalski M ACS Biomater Sci Eng. 2023; 9(12):6683-6697.

PMID: 38032398 PMC: 10716822. DOI: 10.1021/acsbiomaterials.3c01214.

References

Fisher M, Henning E, Soegaard N, Esterhai J, Mauck R . Organized nanofibrous scaffolds that mimic the macroscopic and microscopic architecture of the knee meniscus. Acta Biomater. 2012; 9(1):4496-504. PMC: 3508287. DOI: 10.1016/j.actbio.2012.10.018. View

Bhattarai N, Edmondson D, Veiseh O, Matsen F, Zhang M . Electrospun chitosan-based nanofibers and their cellular compatibility. Biomaterials. 2005; 26(31):6176-84. DOI: 10.1016/j.biomaterials.2005.03.027. View

Ayres C, Bowlin G, Henderson S, Taylor L, Shultz J, Alexander J . Modulation of anisotropy in electrospun tissue-engineering scaffolds: Analysis of fiber alignment by the fast Fourier transform. Biomaterials. 2006; 27(32):5524-34. PMC: 2929953. DOI: 10.1016/j.biomaterials.2006.06.014. View

Tonda-Turo C, Ruini F, Ramella M, Boccafoschi F, Gentile P, Gioffredi E . Non-covalently crosslinked chitosan nanofibrous mats prepared by electrospinning as substrates for soft tissue regeneration. Carbohydr Polym. 2017; 162:82-92. DOI: 10.1016/j.carbpol.2017.01.050. View

Ayres C, Jha B, Meredith H, Bowman J, Bowlin G, Henderson S . Measuring fiber alignment in electrospun scaffolds: a user's guide to the 2D fast Fourier transform approach. J Biomater Sci Polym Ed. 2008; 19(5):603-21. DOI: 10.1163/156856208784089643. View

Nathan A, Baker B, Nerurkar N, Mauck R . Mechano-topographic modulation of stem cell nuclear shape on nanofibrous scaffolds. Acta Biomater. 2010; 7(1):57-66. PMC: 2967658. DOI: 10.1016/j.actbio.2010.08.007. View

Alexander J, Fuss B, Colello R . Electric field-induced astrocyte alignment directs neurite outgrowth. Neuron Glia Biol. 2008; 2(2):93-103. PMC: 2367316. DOI: 10.1017/S1740925X0600010X. View

Kadler K . Matrix loading: assembly of extracellular matrix collagen fibrils during embryogenesis. Birth Defects Res C Embryo Today. 2004; 72(1):1-11. DOI: 10.1002/bdrc.20002. View

Kim Y, Ko H, Kwon I, Kwon I, Shin K . Extracellular Matrix Revisited: Roles in Tissue Engineering. Int Neurourol J. 2016; 20(Suppl 1):S23-29. PMC: 4895908. DOI: 10.5213/inj.1632600.318. View

10.

von Recum A, van Kooten T . The influence of micro-topography on cellular response and the implications for silicone implants. J Biomater Sci Polym Ed. 1995; 7(2):181-98. DOI: 10.1163/156856295x00698. View

11.

Dalby M, Riehle M, Johnstone H, Affrossman S, Curtis A . In vitro reaction of endothelial cells to polymer demixed nanotopography. Biomaterials. 2002; 23(14):2945-54. DOI: 10.1016/s0142-9612(01)00424-0. View

12.

Li H, Xu Y, Xu H, Chang J . Electrospun membranes: control of the structure and structure related applications in tissue regeneration and drug delivery. J Mater Chem B. 2020; 2(34):5492-5510. DOI: 10.1039/c4tb00913d. View

13.

Sinha R, Morris F, Shah S, Tuan R . Surface composition of orthopaedic implant metals regulates cell attachment, spreading, and cytoskeletal organization of primary human osteoblasts in vitro. Clin Orthop Relat Res. 1994; (305):258-72. View

14.

McClure M, Sell S, Ayres C, Simpson D, Bowlin G . Electrospinning-aligned and random polydioxanone-polycaprolactone-silk fibroin-blended scaffolds: geometry for a vascular matrix. Biomed Mater. 2009; 4(5):055010. DOI: 10.1088/1748-6041/4/5/055010. View

15.

Kim J, Hwang T, Aguilar L, Park C, Sang Kim C . A Controlled Design of Aligned and Random Nanofibers for 3D Bi-functionalized Nerve Conduits Fabricated via a Novel Electrospinning Set-up. Sci Rep. 2016; 6:23761. PMC: 4810461. DOI: 10.1038/srep23761. View

16.

Ma B, Xie J, Jiang J, Shuler F, Bartlett D . Rational design of nanofiber scaffolds for orthopedic tissue repair and regeneration. Nanomedicine (Lond). 2013; 8(9):1459-81. PMC: 3875778. DOI: 10.2217/nnm.13.132. View

17.

Baker B, Mauck R . The effect of nanofiber alignment on the maturation of engineered meniscus constructs. Biomaterials. 2007; 28(11):1967-77. PMC: 1847368. DOI: 10.1016/j.biomaterials.2007.01.004. View

18.

Salvatore L, Carofiglio V, Stufano P, Bonfrate V, Calo E, Scarlino S . Potential of Electrospun Poly(3-hydroxybutyrate)/Collagen Blends for Tissue Engineering Applications. J Healthc Eng. 2018; 2018:6573947. PMC: 5933042. DOI: 10.1155/2018/6573947. View

19.

Klossner R, Queen H, Coughlin A, Krause W . Correlation of chitosan's rheological properties and its ability to electrospin. Biomacromolecules. 2008; 9(10):2947-53. DOI: 10.1021/bm800738u. View

20.

Franchi M, Trire A, Quaranta M, Orsini E, Ottani V . Collagen structure of tendon relates to function. ScientificWorldJournal. 2007; 7:404-20. PMC: 5901217. DOI: 10.1100/tsw.2007.92. View