Impact of Ileal Transposition Surgical Intervention on Antioxidant Status Measured in Liver Tissue of Obese Zucker Rats (Crl:ZUC-Lepr)

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2018 Dec 12

PMID 30534350

Citations 3

Authors

Bronislawa Skrzep-Poloczek

Dominika Stygar

Tomasz Sawczyn

Ewa Romuk

Elzbieta Chelmecka

Jakub Poloczek

Agnieszka Dulska

Wojciech Kazura

Konrad W Karcz

Affiliations

Soon will be listed here.

Abstract

Background: The main factor characteristic for low-grade systemic inflammation typical for obesity is oxidative stress (OS). Reactive oxygen species (ROS) production is higher and more increased in time in the obese patients than in lean subjects.

Aims: To assess the effect of ileal transposition (IT) and sham types of bariatric procedures on the antioxidative systems in the liver tissue of Zucker rats (Crl:ZUC Lepr).

Method: 21 animals were divided into the experimental groups: control group ( = 7), sham group ( = 7), and IT group ( = 7). Sham and IT animals underwent selected surgery. The concentration of total antioxidant capacity (TAC), total antioxidant status (TOS), and activity of glutathione reductase (glutathione-disulfide reductase, GR, GSR), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and total superoxide dismutase activity (SOD) were assessed in liver tissue 3 months after surgery.

Results: IT procedure significantly increased TAC when compared to sham and the control group. Animals after IT showed higher levels of TOS when compared to sham procedure. The total amount of TOS was similar in IT and control groups. GPx activity was increased in the groups submitted to the sham and IT surgery in relation to control. GR and CAT activities were significantly higher after IT in comparison to control and sham procedures. Total SOD and MnSOD were significantly higher in sham-operated animals in comparison to IT intervention and control groups.

Conclusions: IT procedure had a positive impact on the diminishing of oxidative stress measured by TAC and TOS markers. The dynamic, adaptive, and protective mechanisms of enzymatic antioxidant systems were observed after the IT but not sham procedure. Nevertheless, 3 months after surgery, the midterm effect of bariatric surgery was observed, which might not fully balance the antioxidative response.

Citing Articles

Reverse Transposition - a New Surgical Model for the Study the Role of Ileum in Glucose Metabolism.

Sevastyanov L, Turgunov E, Shestakov D Bull Exp Biol Med. 2022; 172(5):608-611.

PMID: 35352251 DOI: 10.1007/s10517-022-05442-1.

General, 21-Day Postoperative Rehabilitation Program Has Beneficial Effect on Oxidative Stress Markers in Patients after Total Hip or Knee Replacement.

Skrzep-Poloczek B, Poloczek J, Chelmecka E, Kazura W, Dulska A, Idzik M Oxid Med Cell Longev. 2020; 2020:4598437.

PMID: 33062140 PMC: 7532996. DOI: 10.1155/2020/4598437.

The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery.

Skrzep-Poloczek B, Poloczek J, Chelmecka E, Dulska A, Romuk E, Idzik M Antioxidants (Basel). 2020; 9(2).

PMID: 32098399 PMC: 7070542. DOI: 10.3390/antiox9020183.

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