Molecular Detection of and Genes Among Vancomycin-resistant Enterococci in ICU-hospitalized Patients in Ahvaz in Southwest of Iran

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2018 Dec 12

PMID 30532561

Citations 13

Authors

Mojtaba Moosavian

Hosein Ghadri

Zahra Samli

Affiliations

Soon will be listed here.

Abstract

Objective: Nosocomial infections due to vancomycin-resistant enterococci (VRE) are known as a source of spreading these bacteria. The aim of this prospective study was molecular detection of and genes among VRE isolated from patients admitted to intensive care units (ICUs) in Ahvaz in southwest of Iran.

Materials And Methods: Overall, 243 non-duplicate rectal swab specimens were collected from ICU-hospitalized patients in teaching hospitals affiliated to Ahvaz Jundishapur University of Medical Sciences, Iran. The specimens were inoculated on suitable culture media, and isolates were identified by standard biochemical tests. The susceptibility and resistance of enterococci to 10 antibiotics were determined based on the Clinical and Laboratory Standards Institute guidelines. Resistance to vancomycin was phenotypically detected by vancomycin screening test, and the and genes in vancomycin-resistant isolates were amplified by multiplex PCR method.

Results: Of 175 specimens containing enterococci, 129 (73.7%) isolates were detected as and and 46 (26.3%) isolates as . The results of susceptibility test showed high rates of resistance to tetracycline, erythromycin, ciprofloxacin, and ampicillin. Moreover, based on this test, out of 129 isolates, 56 (43.4%) were resistant to vancomycin and teicoplanin. Also, among 59 vancomycin-resistant or semi-susceptible isolates, gene was detected in 54 (91.5%) isolates, while none of the isolates had gene.

Conclusion: According to the results of this study, to prevent the spread of vancomycin-resistant strains, especially in nosocomial infections, the susceptibility of isolates should be determined before vancomycin prescription.

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