Overexpression of ScMYBAS1 Alternative Splicing Transcripts Differentially Impacts Biomass Accumulation and Drought Tolerance in Rice Transgenic Plants

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2018 Dec 6

PMID 30517137

Citations 9

Authors

Rafael Favero Peixoto-Junior

Larissa Mara de Andrade

Michael Dos Santos Brito

Paula Macedo Nobile

Alexandre Palma Boer Martins

Samira Domingues Carlin

Rafael Vasconcelos Ribeiro

Maria Helena de Souza Goldman

Joao Felipe Nebo Carlos de Oliveira

Antonio Vargas de Oliveira Figueira

Silvana Creste

Affiliations

Soon will be listed here.

Abstract

Drought is the most significant environmental stress for agricultural production worldwide, and tremendous efforts have been made to improve crop yield under the increasing water scarcity. Transcription factors are major players in the regulation of water stress-related genes in plants. Recently, different MYB transcription factors were characterized for their involvement in drought response. A sugarcane R2R3-MYB gene (ScMYBAS1) and its four alternative forms of transcript (ScMYAS1-2, ScMYBAS1-3, ScMYBAS1-4 and ScMYBAS1-5) were identified in this study. The subcellular localization, in Nicotiniana benthamiana, of the TFs fused in frame with GFP revealed that ScMYBAS1-2-GFP and ScMYBAS1-3-GFP were observed in the nucleus. The overexpression of ScMYBAS1-2 and ScMYBAS1-3 spliced transcripts in rice promoted change in plant growth under both well-watered and drought conditions. The ScMYBAS1-2 and ScMYBAS1-3 transgenic lines revealed a higher relative water content (RWC) compared to the wild type before maximum stress under drought conditions. The ScMYBAS1-2 transgenic lines showed a reduction in biomass (total dry weight). Conversely, ScMYBAS1-3 showed an increased biomass (total dry weight) relative to the wild-type. The overexpression of ScMYBAS1-3 in rice transgenic lines showed involvement with drought tolerance and biomass and, for this reason, was considered a good target for plant transformation, particularly for use in developing genotypes with drought tolerance and biomass accumulation.

Citing Articles

Importance of pre-mRNA splicing and its study tools in plants.

Liu Y, Do S, Huynh H, Li J, Liu Y, Du Z Adv Biotechnol (Singap). 2025; 2(1):4.

PMID: 39883322 PMC: 11740881. DOI: 10.1007/s44307-024-00009-9.

Relevance and regulation of alternative splicing in plant secondary metabolism: current understanding and future directions.

Xu Z, Xiao Y, Guo J, Lv Z, Chen W Hortic Res. 2024; 11(8):uhae173.

PMID: 39135731 PMC: 11317897. DOI: 10.1093/hr/uhae173.

High Overexpression of Leads to Growth Inhibition and Protein Ectopic Localization in Transgenic .

Meng R, Li Z, Kang X, Zhang Y, Wang Y, Ma Y Int J Mol Sci. 2024; 25(11).

PMID: 38892028 PMC: 11172308. DOI: 10.3390/ijms25115840.

TrichomeLess Regulator 3 is required for trichome initial and cuticle biosynthesis in Artemisia annua.

Dong B, Xu Z, Wang X, Li J, Xiao Y, Huang D Mol Hortic. 2024; 4(1):10.

PMID: 38500223 PMC: 10949617. DOI: 10.1186/s43897-024-00085-4.

Alternative Splicing for Contributes to Variable Abiotic Stress Resistance in Transgenic Tobacco.

Wang H, Guo Y, Hao X, Zhang W, Xu Y, He W Genes (Basel). 2023; 14(8).

PMID: 37628601 PMC: 10454811. DOI: 10.3390/genes14081549.

References

Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L . MYB transcription factors in Arabidopsis. Trends Plant Sci. 2010; 15(10):573-81. DOI: 10.1016/j.tplants.2010.06.005. View

Yang A, Dai X, Zhang W . A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot. 2012; 63(7):2541-56. PMC: 3346221. DOI: 10.1093/jxb/err431. View

Lawlor D . Genetic engineering to improve plant performance under drought: physiological evaluation of achievements, limitations, and possibilities. J Exp Bot. 2012; 64(1):83-108. DOI: 10.1093/jxb/ers326. View

Karimi M, Depicker A, Hilson P . Recombinational cloning with plant gateway vectors. Plant Physiol. 2007; 145(4):1144-54. PMC: 2151728. DOI: 10.1104/pp.107.106989. View

Mu R, Cao Y, Liu Y, Lei G, Zou H, Liao Y . An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis. Cell Res. 2009; 19(11):1291-304. DOI: 10.1038/cr.2009.83. View

Hsiao T, OToole J, Yambao E, Turner N . Influence of Osmotic Adjustment on Leaf Rolling and Tissue Death in Rice (Oryza sativa L.). Plant Physiol. 1984; 75(2):338-41. PMC: 1066908. DOI: 10.1104/pp.75.2.338. View

Araki S, Ito M, Soyano T, Nishihama R, Machida Y . Mitotic cyclins stimulate the activity of c-Myb-like factors for transactivation of G2/M phase-specific genes in tobacco. J Biol Chem. 2004; 279(31):32979-88. DOI: 10.1074/jbc.M403171200. View

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

Vettore A, da Silva F, Kemper E, Souza G, da Silva A, Ferro M . Analysis and functional annotation of an expressed sequence tag collection for tropical crop sugarcane. Genome Res. 2003; 13(12):2725-35. PMC: 403815. DOI: 10.1101/gr.1532103. View

10.

Seo P, Xiang F, Qiao M, Park J, Lee Y, Kim S . The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis. Plant Physiol. 2009; 151(1):275-89. PMC: 2735973. DOI: 10.1104/pp.109.144220. View

11.

Stracke R, WERBER M, Weisshaar B . The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol. 2001; 4(5):447-56. DOI: 10.1016/s1369-5266(00)00199-0. View

12.

Guo J, Ling H, Ma J, Chen Y, Su Y, Lin Q . A sugarcane R2R3-MYB transcription factor gene is alternatively spliced during drought stress. Sci Rep. 2017; 7:41922. PMC: 5294458. DOI: 10.1038/srep41922. View

13.

Jain M, Nijhawan A, Tyagi A, Khurana J . Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun. 2006; 345(2):646-51. DOI: 10.1016/j.bbrc.2006.04.140. View

14.

Vinocur B, Altman A . Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr Opin Biotechnol. 2005; 16(2):123-32. DOI: 10.1016/j.copbio.2005.02.001. View

15.

Li J, Li X, Guo L, Lu F, Feng X, He K . A subgroup of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice. J Exp Bot. 2006; 57(6):1263-73. DOI: 10.1093/jxb/erj094. View

16.

Wilkins O, Nahal H, Foong J, Provart N, Campbell M . Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiol. 2008; 149(2):981-93. PMC: 2633813. DOI: 10.1104/pp.108.132795. View

17.

Ito M . Conservation and diversification of three-repeat Myb transcription factors in plants. J Plant Res. 2005; 118(1):61-9. DOI: 10.1007/s10265-005-0192-8. View

18.

Ambawat S, Sharma P, Yadav N, Yadav R . MYB transcription factor genes as regulators for plant responses: an overview. Physiol Mol Biol Plants. 2014; 19(3):307-21. PMC: 3715649. DOI: 10.1007/s12298-013-0179-1. View

19.

Seki M, Kamei A, Yamaguchi-Shinozaki K, Shinozaki K . Molecular responses to drought, salinity and frost: common and different paths for plant protection. Curr Opin Biotechnol. 2003; 14(2):194-9. DOI: 10.1016/s0958-1669(03)00030-2. View

20.

Yanhui C, Xiaoyuan Y, Kun H, Meihua L, Jigang L, Zhaofeng G . The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family. Plant Mol Biol. 2006; 60(1):107-24. DOI: 10.1007/s11103-005-2910-y. View