Transcriptome and DNA Methylome Dynamics During Triclosan-Induced Cardiomyocyte Differentiation Toxicity

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2018 Dec 5

PMID 30510588

Citations 5

Authors

Guizhen Du

Mingming Yu

Lingling Wang

Weiyue Hu

Ling Song

Chuncheng Lu

Xinru Wang

Affiliations

Soon will be listed here.

Abstract

Cardiac development is a dynamic process and sensitive to environmental chemicals. Triclosan is widely used as an antibacterial agent and reported to transport across the placenta and affect embryonic development. Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to determine the effects of TCS exposure on cardiac development. After TCS treatment, the differentiation process was significantly blocked and spontaneous beating rates of CMs were also decreased. Transcriptome analysis showed the dysregulation of genes involved in cardiogenesis, including GATA4 and TNNT2. Additionally, DNA methylation was also altered by TCS exposure, especially in those regions with GATA motif enrichment. These alterations of transcriptome and DNA methylation were all associated with signaling pathways integral to heart development. Our findings indicate that TCS exposure might cause cardiomyocyte differentiation toxicity and provide the new insights into how environmental factors regulate DNA methylation and gene expressions during heart development.

Citing Articles

Methylation Partially Mediated the Association of Exposure to Triclosan but Not Triclocarban with Type 2 Diabetes Mellitus: A Case-Control Study.

Gao Q, Huan C, Jia Z, Cao Q, Yuan P, Li X Int J Mol Sci. 2024; 25(22).

PMID: 39596180 PMC: 11594987. DOI: 10.3390/ijms252212113.

Association of same-day urinary phenol levels and cardiac electrical alterations: analysis of the Fernald Community Cohort.

Rubinstein J, Pinney S, Xie C, Wang H Environ Health. 2024; 23(1):76.

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Association of same-day urinary phenol levels and cardiac electrical alterations: analysis of the Fernald Community Cohort.

Rubinstein J, Pinney S, Xie C, Wang H Res Sq. 2024; .

PMID: 38853936 PMC: 11160919. DOI: 10.21203/rs.3.rs-4445657/v1.

Triclosan and Its Consequences on the Reproductive, Cardiovascular and Thyroid Levels.

Marques A, Mariana M, Cairrao E Int J Mol Sci. 2022; 23(19).

PMID: 36232730 PMC: 9570035. DOI: 10.3390/ijms231911427.

Expression of fibroblast growth factor receptor1, -2c, and -3c transcripts in mouse molars after tooth eruption.

Kano T, Morita T, Sumida K, Yumoto H, Baba O Anat Sci Int. 2021; 96(2):301-309.

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