Study of Gene Expression in Colorectal Cancer

Overview

Journal J Gastrointest Oncol

Date 2018 Dec 4

PMID 30505577

Citations 9

Authors

Samira Mousavi

Reza Safaralizadeh

Mohammadali Hosseinpour-Feizi

Arash Azimzadeh-Isfanjani

Shahryar Hashemzadeh

Affiliations

Soon will be listed here.

Abstract

Background: Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide. Cofilin is a key regulatory protein in the dynamics of actin filaments. Previous studies have shown cofilin 1's major role in cell migration process and its role in tumor cell migration and invasion. Therefore, cofilin 1 may have the potential as a novel diagnostic tumor marker in various cancers. In this study, differential expression of CFL1 in CRC tissues in comparison with adjacent non-tumor tissues was investigated and the diagnostic value of this protein in CRC was evaluated.

Methods: Synthesized cDNA from extracted RNAs of 30 patients were subjected to qRT-PCR to quantify relative expression of cofilin 1. The relationship between cofilin 1 expression and clinicopathological features of patients were studied too.

Results: The study showed significant upregulation of cofilin 1 in CRC tissue samples compared to adjacent non-tumor tissue samples (P<0.05). The receiver operating characteristic curve analysis showed higher area under the curve (0.85). There was no significant correlation between cofilin 1 expression levels and clinicopathological features of patients.

Conclusions: According to the obtained results, cofilin 1 can serve as a candidate for clinically useful diagnostic biomarker or therapeutic target for CRC.

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References

Shroff J, Thosani N, Batra S, Singh H, Guha S . Reduced incidence and mortality from colorectal cancer with flexible-sigmoidoscopy screening: a meta-analysis. World J Gastroenterol. 2015; 20(48):18466-76. PMC: 4277988. DOI: 10.3748/wjg.v20.i48.18466. View

Wang W, Mouneimne G, Sidani M, Wyckoff J, Chen X, Makris A . The activity status of cofilin is directly related to invasion, intravasation, and metastasis of mammary tumors. J Cell Biol. 2006; 173(3):395-404. PMC: 2063840. DOI: 10.1083/jcb.200510115. View

Yap C, Simpson T, Pratt T, Price D, Maciver S . The motility of glioblastoma tumour cells is modulated by intracellular cofilin expression in a concentration-dependent manner. Cell Motil Cytoskeleton. 2005; 60(3):153-65. DOI: 10.1002/cm.20053. View

Wang Y, Kuramitsu Y, Ueno T, Suzuki N, Yoshino S, Iizuka N . Differential expression of up-regulated cofilin-1 and down-regulated cofilin-2 characteristic of pancreatic cancer tissues. Oncol Rep. 2011; 26(6):1595-9. DOI: 10.3892/or.2011.1447. View

Nagaoka R, Abe H, Kusano K, Obinata T . Concentration of cofilin, a small actin-binding protein, at the cleavage furrow during cytokinesis. Cell Motil Cytoskeleton. 1995; 30(1):1-7. DOI: 10.1002/cm.970300102. View

Klamt F, Zdanov S, Levine R, Pariser A, Zhang Y, Zhang B . Oxidant-induced apoptosis is mediated by oxidation of the actin-regulatory protein cofilin. Nat Cell Biol. 2009; 11(10):1241-6. PMC: 3393095. DOI: 10.1038/ncb1968. View

Ghaffarzadehgan K, Jafarzadeh M, Raziee H, Sima H, Esmaili-Shandiz E, Hosseinnezhad H . Expression of cell adhesion molecule CD44 in gastric adenocarcinoma and its prognostic importance. World J Gastroenterol. 2008; 14(41):6376-81. PMC: 2766121. DOI: 10.3748/wjg.14.6376. View

Zhou J, Wang Y, Fei J, Zhang W . Expression of cofilin 1 is positively correlated with the differentiation of human epithelial ovarian cancer. Oncol Lett. 2012; 4(6):1187-1190. PMC: 3506761. DOI: 10.3892/ol.2012.897. View

Kim Y, Choi S, Choi M, Oh M, Lee S, Cho M . Cell adhesion-dependent cofilin serine 3 phosphorylation by the integrin-linked kinase.c-Src complex. J Biol Chem. 2008; 283(15):10089-96. DOI: 10.1074/jbc.M708300200. View

10.

Lu L, Fu N, Luo X, Li X, Li X . Overexpression of cofilin 1 in prostate cancer and the corresponding clinical implications. Oncol Lett. 2015; 9(6):2757-2761. PMC: 4473706. DOI: 10.3892/ol.2015.3133. View

11.

Zheng Y, Fang Y, Li S, Zheng B . [Detection of plasma cofilin protein for diagnosis of lung cancer]. Nan Fang Yi Ke Da Xue Xue Bao. 2013; 33(10):1551-3. View

12.

Bamburg J, McGough A, Ono S . Putting a new twist on actin: ADF/cofilins modulate actin dynamics. Trends Cell Biol. 1999; 9(9):364-70. DOI: 10.1016/s0962-8924(99)01619-0. View

13.

Steller E, Ritsma L, Raats D, Hoogwater F, Emmink B, Govaert K . The death receptor CD95 activates the cofilin pathway to stimulate tumour cell invasion. EMBO Rep. 2011; 12(9):931-7. PMC: 3166455. DOI: 10.1038/embor.2011.129. View

14.

van Rheenen J, Condeelis J, Glogauer M . A common cofilin activity cycle in invasive tumor cells and inflammatory cells. J Cell Sci. 2009; 122(Pt 3):305-11. PMC: 2772875. DOI: 10.1242/jcs.031146. View

15.

Collazo J, Zhu B, Larkin S, Martin S, Pu H, Horbinski C . Cofilin drives cell-invasive and metastatic responses to TGF-β in prostate cancer. Cancer Res. 2014; 74(8):2362-73. PMC: 4488067. DOI: 10.1158/0008-5472.CAN-13-3058. View

16.

Zhang Y, Tong X . Expression of the actin-binding proteins indicates that cofilin and fascin are related to breast tumour size. J Int Med Res. 2010; 38(3):1042-8. DOI: 10.1177/147323001003800331. View

17.

Okreglak V, Drubin D . Cofilin recruitment and function during actin-mediated endocytosis dictated by actin nucleotide state. J Cell Biol. 2007; 178(7):1251-64. PMC: 2064657. DOI: 10.1083/jcb.200703092. View

18.

Tsai C, Lin L, Wang C, Chiu Y, Chou Y, Chiu S . Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA. Biochim Biophys Acta. 2015; 1852(5):851-61. DOI: 10.1016/j.bbadis.2015.01.007. View

19.

Muller C, de Barros R, Castro M, Lopes F, Meurer R, Roehe A . Validation of cofilin-1 as a biomarker in non-small cell lung cancer: application of quantitative method in a retrospective cohort. J Cancer Res Clin Oncol. 2011; 137(9):1309-16. DOI: 10.1007/s00432-011-1001-5. View

20.

Ghosh M, Song X, Mouneimne G, Sidani M, Lawrence D, Condeelis J . Cofilin promotes actin polymerization and defines the direction of cell motility. Science. 2004; 304(5671):743-6. DOI: 10.1126/science.1094561. View