BAR Scaffolds Drive Membrane Fission by Crowding Disordered Domains

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2018 Dec 4

PMID 30504247

Citations 35

Authors

Wilton T Snead

Wade F Zeno

Grace Kago

Ryan W Perkins

J Blair Richter

Chi Zhao

Eileen M Lafer

Jeanne C Stachowiak

Affiliations

Soon will be listed here.

Abstract

Cellular membranes are continuously remodeled. The crescent-shaped bin-amphiphysin-rvs (BAR) domains remodel membranes in multiple cellular pathways. Based on studies of isolated BAR domains in vitro, the current paradigm is that BAR domain-containing proteins polymerize into cylindrical scaffolds that stabilize lipid tubules. But in nature, proteins that contain BAR domains often also contain large intrinsically disordered regions. Using in vitro and live cell assays, here we show that full-length BAR domain-containing proteins, rather than stabilizing membrane tubules, are instead surprisingly potent drivers of membrane fission. Specifically, when BAR scaffolds assemble at membrane surfaces, their bulky disordered domains become crowded, generating steric pressure that destabilizes lipid tubules. More broadly, we observe this behavior with BAR domains that have a range of curvatures. These data suggest that the ability to concentrate disordered domains is a key driver of membrane remodeling and fission by BAR domain-containing proteins.

Citing Articles

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Protein-membrane interactions: sensing and generating curvature.

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The ins and outs of membrane bending by intrinsically disordered proteins.

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