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Different Efflux Transporter Affinity and Metabolism of Tc-2-Methoxyisobutylisonitrile and Tc-Tetrofosmin for Multidrug Resistance Monitoring in Cancer

Overview
Journal Pharm Res
Specialties Pharmacology
Pharmacy
Date 2018 Dec 1
PMID 30498970
Citations 4
Authors
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Abstract

Background: Little is known about the affinity and stability of Tc-labeled 2-methoxyisobutylisonitrile (Tc-MIBI) and tetrofosmin (Tc-TF) for imaging of multiple drug resistance transporters in cancer. We examined the affinity of Tc-labeled compounds for these transporters and their stability.

Methods: Tc-MIBI and Tc-TF were incubated in vesicles expressing P-glycoprotein (MDR1), multidrug resistance-associated protein (MRP)1-4, or breast cancer resistance protein with and without verapamil (MDR1 inhibitor) or MK-571 (MRP inhibitor). Time activity curves of Tc-labeled compounds were established using SK-N-SH neuroblastoma, SK-MEL-28 melanoma, and PC-3 prostate adenocarcinoma cell lines, and transporter expression of multiple drug resistance was measured in these cells. The stability was evaluated.

Results: In vesicles, Tc-labeled compounds had affinity for MDR1 and MRP1. Tc-TF had additional affinity for MRP2 and MRP3. In SK-N-SH cells expressing MDR1 and MRP1, MK-571 produced the highest uptake of both Tc-labeled compounds. Tc-MIBI uptake with inhibitors was higher than Tc-TF uptake with inhibitors. Tc-TF was taken up more in SK-MEL-28 cells expressing MRP1 and MRP2 than PC-3 cells expressing MRP1 and MRP3. Tc-MIBI was metabolized, whereas Tc-TF had high stability.

Conclusion: Tc-MIBI is exported via MDR1 and MRP1 (MRP1 > MDR1) at greater levels and more quickly compared to Tc-TF, which is exported via MDR1 and MRP1-3 (MRP1 > MDR1; MRP1, 2 > MRP3). Because Tc-MIBI is metabolized, clinical imaging for monitoring MDR and shorter examination times may be possible with an earlier scanning time on late phase imaging. Tc-TF has high stability and accurately reflects the function of MDR1 and MRP1-3.

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