Hypothermic Oxygenated Machine Perfusion of the Human Donor Pancreas

Overview

Journal Transplant Direct

Publisher Wolters Kluwer

Specialty General Surgery

Date 2018 Dec 1

PMID 30498765

Citations 22

Authors

Marjolein Leemkuil

Grietje Lier

Marten A Engelse

Rutger J Ploeg

Eelco J P de Koning

Nils A t Hart

Christina Krikke

Henri G D Leuvenink

Affiliations

Soon will be listed here.

Abstract

Background: Transplantation of beta cells by pancreas or islet transplantation is the treatment of choice for a selected group of patients suffering from type 1 diabetes mellitus. Pancreata are frequently not accepted for transplantation, because of the relatively high vulnerability of these organs to ischemic injury. In this study, we evaluated the effects of hypothermic machine perfusion (HMP) on the quality of human pancreas grafts.

Methods: Five pancreata derived from donation after circulatory death (DCD) and 5 from donation after brain death (DBD) donors were preserved by oxygenated HMP. Hypothermic machine perfusion was performed for 6 hours at 25 mm Hg by separate perfusion of the mesenteric superior artery and the splenic artery. Results were compared with those of 10 pancreata preserved by static cold storage.

Results: During HMP, homogeneous perfusion of the pancreas could be achieved. Adenosine 5'-triphosphate concentration increased 6,8-fold in DCD and 2,6-fold in DBD pancreata. No signs of cellular injury, edema or formation of reactive oxygen species were observed. Islets of Langerhans with good viability and in vitro function could be isolated after HMP.

Conclusions: Oxygenated HMP is a feasible and safe preservation method for the human pancreas that increases tissue viability.

Citing Articles

Normothermic Machine Perfusion of Explanted Human Metabolic Livers: A Proof of Concept for Studying Inborn Errors of Metabolism.

Safarikia S, Cirelli R, Spagnoletti G, Martinelli D, Bravetti G, Francalanci P J Inherit Metab Dis. 2025; 48(2):e70010.

PMID: 40026238 PMC: 11874047. DOI: 10.1002/jimd.70010.

[Machine perfusion in transplantation surgery].

Kneifel F, Vondran F, Vogel T Chirurgie (Heidelb). 2024; 95(8):610-617.

PMID: 39052038 DOI: 10.1007/s00104-024-02122-w.

Exploring Preservation Modalities in a Split Human Pancreas Model to Investigate the Effect on the Islet Isolation Outcomes.

Buemi A, Mourad N, Bouzin C, Devresse A, Hoton D, Daumerie A Transplant Direct. 2024; 10(7):e1654.

PMID: 38881744 PMC: 11177812. DOI: 10.1097/TXD.0000000000001654.

Ex Vivo Perfusion of Porcine Pancreas and Liver Sourced from Commercial Abattoirs after Circulatory Death as a Research Resource: A Methodological Study.

Rai Z, Magbagbeola M, Doyle K, Lindenroth L, Dwyer G, Gander A Methods Protoc. 2023; 6(4).

PMID: 37489433 PMC: 10366747. DOI: 10.3390/mps6040066.

Sterile Pancreas Inflammation during Preservation and after Transplantation.

Kervella D, Mesnard B, Prudhomme T, Bruneau S, Masset C, Cantarovich D Int J Mol Sci. 2023; 24(5).

PMID: 36902067 PMC: 10003374. DOI: 10.3390/ijms24054636.

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