Sp. Nov., a Bacterium Isolated from an Arable Soil Sample of L

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated strain L4-6, was isolated from an arable soil sample of tobacco in Huize, south-western China and subjected to polyphasic taxonomic characterization. The cells showed oxidase-positive and catalase-positive reactions. Growth occurred at 20-35 °C, at pH 5.0-8.0 and with 0-2 % (w/v) NaCl, optimally at 30 °C, pH 6.0-7.0 and 0-1 % (w/v) NaCl. The major respiratory lipoquinone was ubiquinone-8. The predominant cellular fatty acids (>10.0 %) were identified as summed feature 9 (iso-Cω9 or C 10-methyl), iso-C and iso-C. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, two unidentified aminophospholipids and five unidentified aminolipids. The genomic DNA G+C content was 60.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain L4-6 should be affiliated to the genus and formed a clade with the most closely related organism JS12-10. 16S rRNA gene sequences similarity analysis showed that strain L4-6 was mostly closely related to JS12-10 (98.73 %) and DHOB07 (98.02 %). DNA-DNA hybridization data indicated that strain L4-6 represented a novel genomic species belonging to the genus . The polyphasic taxonomic characteristics indicated that the strain L4-6 represents a novel species of the genus , for which the name sp. nov. (type strain L4-6=CGMCC 1.16273=KCTC 62035) is proposed.