Evaluation and Optimization of a Conventional SPCE for FMD Post-vaccination Monitoring

Overview

Journal BMC Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2018 Nov 30

PMID 30486820

Citations 2

Authors

Yeong-Lim Kang

Ji-Yun Jeong

Hwi-Yeon Choi

Yanhong Zhang

Yumei Liu

Ho-Jong Lee

Jong-Chul Choi

So-Hyun Lee

Beom-Joo Lee

Sang-Won Lee

Joong-Bok Lee

Ki-Hyun Cho

Seung-Yong Park

Affiliations

Soon will be listed here.

Abstract

Background: Foot-and-mouth disease (FMD) can be controlled by either stamping out or vaccination, a choice which depends on both the economic importance of the livestock sector as well as the disease status. In FMD-free countries with vaccination, such as Korea, vaccination programs should guarantee prevention against transmission of FMD. Monitoring of vaccination programs is also essential for ensuring sufficient coverage that will limit the transmission of FMDV. There are several methods to screen FMD virus (FMDV) structural protein (SP) antibodies including SPCE (Solid-phase competitive ELISA), LPBE (Liquid-phase blocking ELISA), and VNT (Virus neutralization test). Among these, SPCE is widely used for serological monitoring since VNT-the gold standard method-has certain practical limitations, such as high costs in terms of time and labor. However, whether SPCE can ensure the vaccination status of individual animals and whole farms is unclear. In this study, SPCE, LPBE and VNT were compared with respect to correlation with each other and sensitivity at commercial pig farms.

Results: The positive results obtained by PrioCHECK SPCE differed from those obtained by LPBE and VNT. The sensitivity of SPCE relative to those of the other tests was fairly low. The raw data of SPCE were most highly correlated with those of VNT with XJ strain, while their positivity and negativity were most highly correlated with LPBE. The results of ROC analysis proposed new cut-off for PrioCHECK SPCE higher than the previous 50% inhibition.

Conclusions: The high false positive rate of PrioCHECK SPCE suggested that high seropositivity by SPCE may not guarantee a true vaccination coverage. Adjusting the cut-off percentage (%) inhibition value for SPCE is needed to address this problem, and it is highly recommended that routine FMDV serological monitoring programs using PrioCHECK SPCE should be combined with alternative methods such as LPBE or VNT.

Citing Articles

Advances in the Diagnosis of Foot-and-Mouth Disease.

Wong C, Yong C, Ong H, Ho K, Tan W Front Vet Sci. 2020; 7:477.

PMID: 32974392 PMC: 7473413. DOI: 10.3389/fvets.2020.00477.

Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis.

Nguyen Q, Yang J, Byun J, Pyo H, Park M, Ku B Pathogens. 2019; 8(4).

PMID: 31861046 PMC: 6963590. DOI: 10.3390/pathogens8040301.

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