Detection of JC Polyomavirus Tumor Antigen in Gastric Carcinoma: a Report from Iran

Overview

Journal Iran J Microbiol

Publisher Tehran University of Medical Sciences

Specialty Infectious Diseases

Date 2018 Nov 29

PMID 30483380

Citations 2

Authors

Samira Izi

Masoud Youssefi

Farzad Rahmani

Nema Mohammadian Roshan

Atefeh Yari

Farnaz Zahedi Avval

Affiliations

Soon will be listed here.

Abstract

Background And Objectives: Factors contributing to development of gastric cancer are still under investigation. The JC Virus (JCV), as an oncogenic virus, has been indicated to play a possible role in gastric carcinogenesis. Theoretically, tumor antigen (T-Ag), the viral transforming protein, is capable of binding and inactivating tumor suppressor proteins p53 and pRb, there by promoting cancer development although such a role in gastric cancer is still controversial and additional data is needed to reach a definite conclusion. The prevalence of the virus varies in different geographic regions, therefore, we aimed to investigate JCV presence in cancerous gastric tissues of Iranian patients.

Materials And Methods: Thirty-one paired samples were included in this study (total of 62 samples). T-Ag sequences were investigated using real-time PCR in formalin fixed paraffin embedded (FFPE) tissue samples from the tumor site and relevant adjacent non-cancerous tissues (ANCT). In positive samples, JCV copy number (viral load) was also measured using real-time PCR. To evaluate T-Ag protein expression, immunohistochemistry examination was performed using an anti-T-Ag specific antibody.

Results: JCV sequences were detected in 17 out of 31 gastric cancer tissue samples (54.84%) and in 10 out of 31 of the non-cancerous adjacent gastric mucosa (32.25%) (Odds ratio of 2.4). Viral load in tumoral and adjacent tissue samples was not statistically different (p=0.88). Immunohistochemical study confirmed presence of JC T-Ag in the nuclear compartment.

Conclusion: We showed the presence of the JC virus in gastric carcinoma tissue samples in our geographic region. This finding provides supportive data for a possible contribution of JCV in gastric cell transformation to malignancy. However, we highly recommend additional investigations to further explore JC virus and gastric cancer in order to reach a conclusion.

Citing Articles

Viral Pathogens in Oesophageal and Gastric Cancer.

Rajendra K, Sharma P Pathogens. 2022; 11(4).

PMID: 35456151 PMC: 9029269. DOI: 10.3390/pathogens11040476.

Associations Between Gastric Cancer Risk and Virus Infection Other Than Epstein-Barr Virus: A Systematic Review and Meta-analysis Based on Epidemiological Studies.

Wang H, Chen X, Liu K, Bai D, Zhang W, Chen X Clin Transl Gastroenterol. 2020; 11(7):e00201.

PMID: 32764207 PMC: 7386361. DOI: 10.14309/ctg.0000000000000201.

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