Nicotinic Acetylcholine Receptor α6 Subunit Mutation (G275V) Found in a Spinosad-resistant Strain of the Flower Thrips, (Thysanoptera: Thripidae)

Overview

Journal J Pestic Sci

Date 2018 Nov 28

PMID 30479549

Citations 4

Authors

Emika Hiruta

Misato Aizawa

Akio Nakano

Shoji Sonoda

Affiliations

Soon will be listed here.

Abstract

The flower thrips strain showing resistance to spinosad was established in the laboratory. The resistant strain showed an LC value of 1398.7 mg/L in a leaf dipping/contact assay. The LC value was 280 times higher than that of the most susceptible strain. An insecticidal assay using synergists suggested no involvement of degradation enzymes, such as cytochrome P450, glutathione -transferase, and carboxyl esterase, in the resistance. Glycine at amino acid position 275 of the nicotinic acetylcholine receptor (nAChR) α6 subunit was mutated to valine in the resistant strain. These results suggest that spinosad resistance in is conferred by the reduced sensitivity of nAChR.

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References

Wang J, Wang X, Lansdell S, Zhang J, Millar N, Wu Y . A three amino acid deletion in the transmembrane domain of the nicotinic acetylcholine receptor α6 subunit confers high-level resistance to spinosad in Plutella xylostella. Insect Biochem Mol Biol. 2016; 71:29-36. PMC: 4821178. DOI: 10.1016/j.ibmb.2016.02.001. View

Hsu J, Feng H, Wu W, Geib S, Mao C, Vontas J . Truncated transcripts of nicotinic acetylcholine subunit gene Bdα6 are associated with spinosad resistance in Bactrocera dorsalis. Insect Biochem Mol Biol. 2012; 42(10):806-15. DOI: 10.1016/j.ibmb.2012.07.010. View

Hou W, Liu Q, Tian L, Wu Q, Zhang Y, Xie W . The α6 nicotinic acetylcholine receptor subunit of Frankliniella occidentalis is not involved in resistance to spinosad. Pestic Biochem Physiol. 2014; 111:60-7. DOI: 10.1016/j.pestbp.2014.03.004. View

Rinkevich F, Chen M, Shelton A, Scott J . Transcripts of the nicotinic acetylcholine receptor subunit gene Pxylα6 with premature stop codons are associated with spinosad resistance in diamondback moth, Plutella xylostella. Invert Neurosci. 2010; 10(1):25-33. DOI: 10.1007/s10158-010-0102-1. View

Bao W, Narai Y, Nakano A, Kaneda T, Murai T, Sonoda S . Spinosad resistance of melon thrips, Thrips palmi, is conferred by G275E mutation in α6 subunit of nicotinic acetylcholine receptor and cytochrome P450 detoxification. Pestic Biochem Physiol. 2014; 112:51-5. DOI: 10.1016/j.pestbp.2014.04.013. View

Scott J . Cytochromes P450 and insecticide resistance. Insect Biochem Mol Biol. 1999; 29(9):757-77. DOI: 10.1016/s0965-1748(99)00038-7. View

Hemingway J, Hawkes N, McCarroll L, Ranson H . The molecular basis of insecticide resistance in mosquitoes. Insect Biochem Mol Biol. 2004; 34(7):653-65. DOI: 10.1016/j.ibmb.2004.03.018. View

Bielza P, Quinto V, Fernandez E, Gravalos C, Contreras J . Genetics of spinosad resistance in Frankliniella occidentalis (Thysanoptera: Thripidae). J Econ Entomol. 2007; 100(3):916-20. DOI: 10.1603/0022-0493(2007)100[916:gosrif]2.0.co;2. View

Puinean A, Lansdell S, Collins T, Bielza P, Millar N . A nicotinic acetylcholine receptor transmembrane point mutation (G275E) associated with resistance to spinosad in Frankliniella occidentalis. J Neurochem. 2012; 124(5):590-601. PMC: 3644170. DOI: 10.1111/jnc.12029. View

10.

Zhao J, Li Y, Collins H, Mau R, Thompson G, Shelto A . Monitoring and characterization of diamondback moth (Lepidoptera: Plutellidae) resistance to spinosad. J Econ Entomol. 2002; 95(2):430-6. DOI: 10.1603/0022-0493-95.2.430. View

11.

Inoue T, Sakurai T, Murai T, Maeda T . Specificity of accumulation and transmission of tomato spotted wilt virus (TSWV) in two genera, Frankliniella and Thrips (Thysanoptera: Thripidae). Bull Entomol Res. 2004; 94(6):501-7. DOI: 10.1079/ber2004326. View

12.

Feyereisen R . Insect P450 enzymes. Annu Rev Entomol. 1999; 44:507-33. DOI: 10.1146/annurev.ento.44.1.507. View

13.

Baxter S, Chen M, Dawson A, Zhao J, Vogel H, Shelton A . Mis-spliced transcripts of nicotinic acetylcholine receptor alpha6 are associated with field evolved spinosad resistance in Plutella xylostella (L.). PLoS Genet. 2010; 6(1):e1000802. PMC: 2792709. DOI: 10.1371/journal.pgen.1000802. View