Flavonoids Are Identified from the Extract of Scutellariae Radix to Suppress Inflammatory-induced Angiogenic Responses in Cultured RAW 264.7 Macrophages

Overview

Journal Sci Rep

Specialty Science

Date 2018 Nov 28

PMID 30479366

Citations 21

Authors

Guowei Gong

Huaiyou Wang

Xiangpeng Kong

Ran Duan

Tina T X Dong

Karl W K Tsim

Affiliations

Soon will be listed here.

Abstract

Scutellariae Radix (SR), also named Huangqin in China, is the dried root of Scutellaria baicalensis Georgi. Historically, the usage of SR was targeted to against inflammation. In fact, chronic inflammation has a close relationship with hypoxia and abnormal angiogenesis in tumor cells. Hence, we would like to probe the water extract of SR in suppressing the inflammation-induced angiogenesis. Prior to determine the pharmaceutical values of SR, the first step is to analysis the chemical compositions of SR according to China Pharmacopeia (2015). From the results, the amount of baicalin was 12.6% by weight. Furthermore, the anti-angiogenic properties of SR water extract were evaluated in lipopolysaccharide (LPS) pre-treated cultured macrophage RAW 264.7 cells by detecting the inflammatory markers, i.e. Cox-2, cytokine and iNOS, as well as the translocation activity of NFκB and angiogenic biomarker, i.e. VEGF. This herbal extract was capable of declining both inflammatory and angiogenic hallmarks in a concentration-dependent manner. Moreover, the SR-derived flavonoids, i.e. baicalin, baicalein, wogonin and wogonoside, were shown to be active chemicals in the anti-inflammatory-induced angiogenesis. Therefore, the inflammation-induced angiogenesis is believed to be suppressed by SR water extract, or its major ingredients. These results shed light in the benefiting role of SR in the inflammation-induced angiogenesis in vitro.

Citing Articles

Unveiling the power of flavonoids: A dynamic exploration of their impact on cancer through matrix metalloproteinases regulation.

Rajendran P Biomedicine (Taipei). 2024; 14(2):12-28.

PMID: 38939095 PMC: 11204124. DOI: 10.37796/2211-8039.1447.

Ethanol extract of basil ( L.) leaves inhibits endometriosis growth in a mouse model by modulating vascular endothelial growth factor (VEGF) expression.

Ayuni D, Saadi A, Widjiati W J Med Life. 2023; 16(8):1224-1230.

PMID: 38024822 PMC: 10652688. DOI: 10.25122/jml-2023-0225.

Biotechnological strategies for controlled accumulation of flavones in hairy root culture of Scutellaria lateriflora L.

Wilczanska A, Sparzak-Stefanowska B, Kokotkiewicz A, Jesionek A, Krolicka A, Luczkiewicz M Sci Rep. 2023; 13(1):20422.

PMID: 37990031 PMC: 10663461. DOI: 10.1038/s41598-023-47757-7.

Zhuanggu Guanjie herbal formula mitigates osteoarthritis the NF-κB transduction mechanism.

Guowei G, Yuzhong Z, Xuan Z, Zhi D, Juanhui D, Jing W Front Pharmacol. 2022; 13:896397.

PMID: 36532734 PMC: 9751418. DOI: 10.3389/fphar.2022.896397.

Characterization of a macrophagic-like cell line derived from rabbit fish (): An illustration of anti-inflammatory responses of the herbal extract of .

Xia Y, Cheng E, Xia Y, Wu Q, Zhang L, Lin S Fish Shellfish Immunol Rep. 2022; 2:100036.

PMID: 36420515 PMC: 9680100. DOI: 10.1016/j.fsirep.2021.100036.

References

Landskron G, De la Fuente M, Thuwajit P, Thuwajit C, Hermoso M . Chronic inflammation and cytokines in the tumor microenvironment. J Immunol Res. 2014; 2014:149185. PMC: 4036716. DOI: 10.1155/2014/149185. View

Aharonov O, Maftzir G, Benezra D . The role of cytokines in angiogenesis. Ocul Immunol Inflamm. 2012; 1(1-2):135-42. DOI: 10.3109/09273949309086550. View

Gao J, Morgan W, Sanchez-Medina A, Corcoran O . The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells. Toxicol Appl Pharmacol. 2011; 254(3):221-8. DOI: 10.1016/j.taap.2011.03.016. View

Li C, Lin G, Zuo Z . Pharmacological effects and pharmacokinetics properties of Radix Scutellariae and its bioactive flavones. Biopharm Drug Dispos. 2011; 32(8):427-45. DOI: 10.1002/bdd.771. View

Yang Y, Tang Y, Liu Y . Wogonoside displays anti-inflammatory effects through modulating inflammatory mediator expression using RAW264.7 cells. J Ethnopharmacol. 2013; 148(1):271-6. DOI: 10.1016/j.jep.2013.04.025. View

Newton K, Dixit V . Signaling in innate immunity and inflammation. Cold Spring Harb Perspect Biol. 2012; 4(3). PMC: 3282411. DOI: 10.1101/cshperspect.a006049. View

Li H, Chen X, Yang Y, Huang H, Zhang L, Zhang X . Wogonin attenuates inflammation by activating PPAR-γ in alcoholic liver disease. Int Immunopharmacol. 2017; 50:95-106. DOI: 10.1016/j.intimp.2017.06.013. View

Harmey J, Dimitriadis E, Kay E, Redmond H, Bouchier-Hayes D . Regulation of macrophage production of vascular endothelial growth factor (VEGF) by hypoxia and transforming growth factor beta-1. Ann Surg Oncol. 1998; 5(3):271-8. DOI: 10.1007/BF02303785. View

Hamrah P, Chen L, Zhang Q, Dana M . Novel expression of vascular endothelial growth factor receptor (VEGFR)-3 and VEGF-C on corneal dendritic cells. Am J Pathol. 2003; 163(1):57-68. PMC: 1868166. DOI: 10.1016/S0002-9440(10)63630-9. View

10.

Gately S, Li W . Multiple roles of COX-2 in tumor angiogenesis: a target for antiangiogenic therapy. Semin Oncol. 2004; 31(2 Suppl 7):2-11. DOI: 10.1053/j.seminoncol.2004.03.040. View

11.

Zhong T, Arancibia S, Born R, Tampe R, Villar J, Del Campo M . Hemocyanins Stimulate Innate Immunity by Inducing Different Temporal Patterns of Proinflammatory Cytokine Expression in Macrophages. J Immunol. 2016; 196(11):4650-62. PMC: 4873724. DOI: 10.4049/jimmunol.1501156. View

12.

Deng Z, Zhang J, Jiao M, Zhong W, Cui W, Cheng J . [Grades evaluation of Scutellariae Radix slices based on quality constant]. Zhongguo Zhong Yao Za Zhi. 2017; 42(9):1673-1678. DOI: 10.19540/j.cnki.cjcmm.20170223.009. View

13.

Howe L, Subbaramaiah K, Brown A, Dannenberg A . Cyclooxygenase-2: a target for the prevention and treatment of breast cancer. Endocr Relat Cancer. 2001; 8(2):97-114. DOI: 10.1677/erc.0.0080097. View

14.

Gong A, Zhang L, Lam C, Xu M, Wang H, Lin H . Polysaccharide of Danggui Buxue Tang, an Ancient Chinese Herbal Decoction, Induces Expression of Pro-inflammatory Cytokines Possibly Via Activation of NFκB Signaling in Cultured RAW 264.7 Cells. Phytother Res. 2016; 31(2):274-283. DOI: 10.1002/ptr.5745. View

15.

Dou J, Wang Z, Ma L, Peng B, Mao K, Li C . Baicalein and baicalin inhibit colon cancer using two distinct fashions of apoptosis and senescence. Oncotarget. 2018; 9(28):20089-20102. PMC: 5929448. DOI: 10.18632/oncotarget.24015. View

16.

Tseng Y, Wu Y, Leu Y, Yeh S, Chou C . Scutellariae radix suppresses hepatitis B virus production in human hepatoma cells. Front Biosci (Elite Ed). 2010; 2(4):1538-47. DOI: 10.2741/e213. View

17.

Coussens L, Werb Z . Inflammation and cancer. Nature. 2002; 420(6917):860-7. PMC: 2803035. DOI: 10.1038/nature01322. View

18.

Zhang L, Xing D, Wang W, Wang R, Du L . Kinetic difference of baicalin in rat blood and cerebral nuclei after intravenous administration of Scutellariae Radix extract. J Ethnopharmacol. 2005; 103(1):120-5. DOI: 10.1016/j.jep.2005.07.013. View

19.

Turner M, Nedjai B, Hurst T, Pennington D . Cytokines and chemokines: At the crossroads of cell signalling and inflammatory disease. Biochim Biophys Acta. 2014; 1843(11):2563-2582. DOI: 10.1016/j.bbamcr.2014.05.014. View

20.

Fan Y, Mao R, Yang J . NF-κB and STAT3 signaling pathways collaboratively link inflammation to cancer. Protein Cell. 2013; 4(3):176-85. PMC: 4875500. DOI: 10.1007/s13238-013-2084-3. View