Architecture and Subunit Arrangement of the Complete Saccharomyces Cerevisiae COMPASS Complex

Overview

Journal Sci Rep

Specialty Science

Date 2018 Nov 28

PMID 30479350

Citations 10

Authors

Yanxing Wang

Zhanyu Ding

Xiangyang Liu

Yu Bao

Min Huang

Catherine C L Wong

Xiaoyu Hong

Yao Cong

Affiliations

Soon will be listed here.

Abstract

Methylation of histone H3 lysine 4 (H3K4) is catalyzed by the multi-component COMPASS or COMPASS-like complex, which is highly conserved from yeast to human, and plays essential roles in gene expression and transcription, cell cycle progression, and DNA repair. Here we present a cryo-EM map of the complete S. cerevisiae COMPASS complex. Through tag or Fab labeling strategy combined with cryo-EM 3D reconstruction and cross-linking and mass spectrometry (XL-MS) analysis, we uncovered new information on the subunit arrangement: Cps50, Cps35, and Cps30 were determined to group together to form the face region in the head of the complex, and Cps40 and the N-terminal portion of Set1 reside on the top of the head. Our map reveals the location of the active center and a canyon in the back of the head. Together, our study provides the first snapshot of the complete architecture of yeast COMPASS and a picture of its subunit interaction network, which could facilitate our understanding of the COMPASS machinery and its functionality.

Citing Articles

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