Anther Culture in Rice Proportionally Rescues Microspores According to Gametophytic Gene Effect and Enhances Genetic Study of Hybrid Sterility

Overview

Journal Plant Methods

Publisher Biomed Central

Specialty Biology

Date 2018 Nov 27

PMID 30473723

Citations 1

Authors

Yoshitaka Kanaoka

Daichi Kuniyoshi

Eri Inada

Yohei Koide

Yoshihiro Okamoto

Hideshi Yasui

Yuji Kishima

Affiliations

Soon will be listed here.

Abstract

Background: To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice () and African rice (). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed loci, but this complicated genetic system remains poorly understood.

Results: Microspores from these interspecific hybrids form sterile pollen but are viable at the immature stage. Application of the anther culture (AC) method caused these immature microspores to induce callus. The segregation distortion of 11 among 13 known loci was assessed in the callus population. Using many individual calli, fine mapping of the loci was attempted based on heterozygotes produced from chromosome segment substitution lines (CSSLs). Transmission ratio distortion (TRD) from microspores was detected at 6 of 11 loci in the callus population. The fine mapping of and loci using CSSLs revealed precise distances of markers from the positions of loci exhibiting excessive TRD.

Conclusions: We demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple loci and dramatically shortened the process for mapping hybrid sterility genes.

Citing Articles

Diploid Male Gametes Circumvent Hybrid Sterility Between Asian and African Rice Species.

Kuniyoshi D, Masuda I, Kanaoka Y, Shimazaki-Kishi Y, Okamoto Y, Yasui H Front Plant Sci. 2020; 11:579305.

PMID: 33224168 PMC: 7674174. DOI: 10.3389/fpls.2020.579305.

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