Carboxy-terminal Domain of Cas Differentially Modulates Expression, DNA Synthesis, and Membrane Ruffling Induced by Insulin, EGF, and IGF-1

Overview

Journal Anim Cells Syst (Seoul)

Specialty Biology

Date 2018 Nov 22

PMID 30460082

Citations 1

Authors

Sun-Ju Yi

Seong Yun Hwang

Myung-Ju Oh

Kyunghwan Kim

Byung H Jhun

Affiliations

Soon will be listed here.

Abstract

p130 Crk-associated substrate (Cas) is an adaptor protein associating with many other signaling proteins and regulates a various biological processes including cell adhesion, migration, and growth factor stimulation. However, the exact functional role of Cas in growth factor signaling pathway was poorly understood. Here we investigated the role of Cas and its domains in the effects of insulin, EGF, and IGF-1 on c-Jun gene expression, DNA synthesis, cytoskeletal reorganization. We found that microinjection of anti-Cas antibody and C-terminal domain of Cas (Cas-CT) specifically inhibited EGF-induced, but not insulin- or IGF-1-induced, c-Jun expression. Cell cycle progression and cytoskeleton reorganization induced by insulin and EGF, but not by IGF-1, were inhibited by microinjected anti-Cas and Cas-CT. In contrast, microinjection of the substate domain (Cas-SD) of Cas did not have any inhibitory effects. These results revealed that the Cas-CT is differentially implicated in insulin and EGF-mediated, but not IGF-1-mediated, c-Jun expression, DNA synthesis and membrane ruffling.

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PMID: 30834155 PMC: 6394304. DOI: 10.1080/19768354.2018.1561516.

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