Pilot Safety Evaluation of a Novel Strain of

Overview

Journal Front Genet

Date 2018 Nov 22

PMID 30459813

Citations 12

Authors

Huizi Tan

Zhiming Yu

Chen Wang

Qingsong Zhang

Jianxin Zhao

Hao Zhang

Qixiao Zhai

Wei Chen

Affiliations

Soon will be listed here.

Abstract

ELH-B2 is considered as a potential next-generation probiotic due to its preventive effects on lipopolysaccharides-associated inflammation and intestinal microbiota disorders in mice. To study safety issues associated with ELH-B2, we conducted comprehensive and systematic experiments, including genetic assessments of potential virulence and antimicrobial resistance genes, and an acute toxicity study of both immunocompetent and immunosuppressed mice via cyclophosphamide treatment. The results indicated that this novel strain is non-toxigenic, fragilysin is not expressed, and most of potential virulence genes are correlated with cellular structures such as capsular polysaccharide and polysaccharide utilizations. The antibiotic resistance features are unlikely be transferred to other intestinal microorganisms as no plasmids nor related genomic islands were identified. Side effects were not observed in mice. ELH-B2 also alleviated the damages caused by cyclophosphamide injection.

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