Species Population Found in Pond Water of Parks in Mashhad City, Can the Physicochemical Factors Affect It?

species are the ubiquitous free-living amoebas that are found worldwide in soil and water. Among spp., can cause primary amebic meningoencephalitis (PAM). Ninety water samples were collected from the pond of parks. Also, the water quality parameters were measured at the sampling site (such as temperature, pH, total dissolved solids (TDS), electrical conductivity (EC) and Turbidity). After filtering, the samples were cultured on Bacto-agar enriched with . A PCR assay was conducted on the culture-positive samples in the ITS1, 5.8SrDNA and ITS2 regions, and then the PCR products were sequenced. The pond water of parks was contaminated with some spp. (except ) and a . There was no significant relationship between water quality parameters and the presence of (p > 0.05). Our protocol investigates to detect spp. from ponds water of parks in Mashhad city and the relations between the water quality parameters and its presence.