Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Lysates

Overview

Journal Bio Protoc

Specialty Biology

Date 2018 Nov 20

PMID 30450365

Citations 5

Authors

Olga Sin

Alejandro Mata-Cabana

Renee I Seinstra

Ellen A A Nollen

Affiliations

Soon will be listed here.

Abstract

Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein's native conformation, which ultimately results in its aggregation or abnormal localization within the cell. Using a model of polyglutamine diseases, we describe in detail the filter retardation assay, a method that captures protein aggregates in a cellulose acetate membrane and allows its detection and quantification by immunoblotting.

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