MiR-224 Is Significantly Upregulated and Targets Caspase-3 and Caspase-7 During Colorectal Carcinogenesis

Overview

Journal Transl Oncol

Specialty Oncology

Date 2018 Nov 19

PMID 30448733

Citations 7

Authors

Matteo Fassan

Ri Cui

Pierluigi Gasparini

Claudia Mescoli

Vincenza Guzzardo

Caterina Vicentini

Giada Munari

Fotios Loupakis

Sara Lonardi

Chiara Braconi

Marco Scarpa

Edoardo DAngelo

Salvatore Pucciarelli

Imerio Angriman

Marco Agostini

Renata DInca

Fabio Farinati

Roberta Gafa

Giovanni Lanza

Wendy L Frankel

Carlo Maria Croce

Nicola Valeri

Massimo Rugge

Affiliations

Soon will be listed here.

Abstract

miR-224 has recently emerged as a driver oncomiR in sporadic colorectal carcinogenesis, but its pathogenetic role is still controversial. A large phenotypical and molecularly characterized series of preinvasive and invasive colorectal lesions was investigated for miR-224 expression by qRT-PCR and in situ hybridization. The caspase-3 and caspase-7 status was also assessed and correlated to miR-224 dysregulation. miR-224 was significantly upregulated during the adenoma-carcinoma sequence and in the context of inflammatory bowel disease dysplastic lesions, whereas its expression was significantly downregulated among BRAF-mutated tumors and in the presence of a DNA mismatch repair deficiency. miR-224 targets caspase-3 and caspase-7 in colorectal cancer, and this inverse relation was already evident from the earliest phases of transformation in intestinal mucosa. The miR-224/caspases axis may represent an interesting field of study for innovative biomarkers/therapeutics for BRAF-mutated/DNA mismatch repair-deficient tumors.

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