Combining 3D Graphene-like Screen-printed Carbon Electrode with Methylene Blue-loaded Liposomal Nanoprobes for Phospholipase A Detection

Overview

Journal Biosens Bioelectron

Specialties Biochemistry
Biotechnology

Date 2018 Nov 17

PMID 30445300

Citations 2

Authors

Yonghua Zhang

Junjie Ai

Yanan Dong

Shiyu Zhang

Qiang Gao

Honglan Qi

Chengxiao Zhang

Zhiliang Cheng

Affiliations

Soon will be listed here.

Abstract

Phospholipase A (PLA) enzyme could be acted as a unique biomarker for forecasting and diagnosing certain diseases. Therefore, it is important to monitor PLA activity in biological and clinical samples. In this work, a simple electrochemical assay for PLA activity was developed based on a screen-printed carbon electrode (SPCE) with 3D graphene-like surface. When the PLA-containing sample was mixed with the nanoprobes, i.e. the electroactive marker methylene blue (MB) encapsulated within nanometer-sized phospholipid liposomes, MB was released and adsorbed/enriched in site onto the surface of SPCE in a micro-cell. The encapsulation and enzymatic release of MB were evaluated using UV-Vis and fluorescence. The peak current due to oxidation of the adsorbed MB on the SPCE was measured by square-wave voltammetry (SWV). The current was directly linear to the PLA activity from 5 U/L to 200 U/L with a detection limit of 3 U/L. The same method can also be used for screening PLA inhibitors. Thus, the enrichment strategy developed in this work could be a promising signal amplification method for the sensitive and selective detection of PLA in biological or clinical samples.

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