'Governor Vessel-unblocking and Mind-regulating' Acupuncture Therapy Ameliorates Cognitive Dysfunction in a Rat Model of Middle Cerebral Artery Occlusion

Overview

Journal Int J Mol Med

Specialty Genetics

Date 2018 Nov 16

PMID 30431067

Citations 18

Authors

Xuan Su

Zuqiang Wu

Fangyong Mai

Zhiyong Fan

Shujia Du

Hong Qian

Jingwen Zhu

Affiliations

Soon will be listed here.

Abstract

Acupuncture is a traditional Chinese medicinal therapy, which is used for the amelioration of cognitive dysfunction. The aim of this study was to investigate the effectiveness and relevancy mechanisms of 'governor vessel‑unblocking and mind‑regulating' acupuncture therapy for cognitive dysfunction in rats with ischemia. For this purpose, we used the middle cerebral artery occlusion (MCAO) method to induce cognitive dysfunction in rats. The behavioral changes in the rats were examined using the Morris water maze (MWM) test. The effects of the treatment on oxidative stress response and the function of the mitochondria in brain tissues were also assessed. The results revealed that 'governor vessel‑unblocking and mind‑regulating' acupuncture therapy markedly improved the cognitive ability of the rats with cognitive dysfunction. The production of pro‑oxidative stress factors, including nitric oxide (NO) and inducible nitric oxide synthase (iNOS), was also blocked along with the amelioration of cognitive function, while the production of adenosine triphosphate (ATP), superoxide dismutase (SOD) and cyclooxygenase (COX) was restored. At the molecular level, the accumulation of amyloid β (Aβ) in the mitochondria was suppressed by 'governor vessel‑unblocking and mind‑regulating' acupuncture therapy, which may be attributed to the inhibition of the function of translocase of outer mitochondrial membrane 40 (TOMM40) and translocase of inner mitochondrial membrane 17A (TIMM17A). On the whole, the findings of the present study confirm the effects of 'governor vessel‑unblocking and mind‑regulating' acupuncture therapy on cognitive dysfunction induced by brain ischemia in rats, and that the mechanisms underlying the effects of this treatment might be mediated through the inhibition of TOMM40 and TIMM17A synthesis, which can relieve mitochondrial dysfunction from the accumulation of Aβ.

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References

Leung M, Yip K, Ho Y, Siu F, Li W, Garner B . Mechanisms underlying the effect of acupuncture on cognitive improvement: a systematic review of animal studies. J Neuroimmune Pharmacol. 2014; 9(4):492-507. DOI: 10.1007/s11481-014-9550-4. View

Strittmatter W, Weisgraber K, Huang D, Dong L, Salvesen G, Pericak-Vance M . Binding of human apolipoprotein E to synthetic amyloid beta peptide: isoform-specific effects and implications for late-onset Alzheimer disease. Proc Natl Acad Sci U S A. 1993; 90(17):8098-102. PMC: 47295. DOI: 10.1073/pnas.90.17.8098. View

Wallace D . Mitochondrial diseases in man and mouse. Science. 1999; 283(5407):1482-8. DOI: 10.1126/science.283.5407.1482. View

Ferencz B, Karlsson S, Kalpouzos G . Promising Genetic Biomarkers of Preclinical Alzheimer's Disease: The Influence of APOE and TOMM40 on Brain Integrity. Int J Alzheimers Dis. 2012; 2012:421452. PMC: 3328927. DOI: 10.1155/2012/421452. View

Wallace D . Mitochondrial genetics: a paradigm for aging and degenerative diseases?. Science. 1992; 256(5057):628-32. DOI: 10.1126/science.1533953. View

Chou P, Chu H, Lin J . Effects of electroacupuncture treatment on impaired cognition and quality of life in Taiwanese stroke patients. J Altern Complement Med. 2010; 15(10):1067-1073. View

Tabner B, Turnbull S, El-Agnaf O, Allsop D . Formation of hydrogen peroxide and hydroxyl radicals from A(beta) and alpha-synuclein as a possible mechanism of cell death in Alzheimer's disease and Parkinson's disease. Free Radic Biol Med. 2002; 32(11):1076-83. DOI: 10.1016/s0891-5849(02)00801-8. View

Caselli R, Dueck A, Huentelman M, Lutz M, Saunders A, Reiman E . Longitudinal modeling of cognitive aging and the TOMM40 effect. Alzheimers Dement. 2012; 8(6):490-5. PMC: 3483561. DOI: 10.1016/j.jalz.2011.11.006. View

Ye S, Huang Y, Mullendorff K, Dong L, Giedt G, Meng E . Apolipoprotein (apo) E4 enhances amyloid beta peptide production in cultured neuronal cells: apoE structure as a potential therapeutic target. Proc Natl Acad Sci U S A. 2005; 102(51):18700-5. PMC: 1311738. DOI: 10.1073/pnas.0508693102. View

10.

Sultana R, Butterfield D . Oxidatively modified, mitochondria-relevant brain proteins in subjects with Alzheimer disease and mild cognitive impairment. J Bioenerg Biomembr. 2009; 41(5):441-6. PMC: 2920455. DOI: 10.1007/s10863-009-9241-7. View

11.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

12.

Bozner P, Grishko V, LeDoux S, Wilson G, Chyan Y, Pappolla M . The amyloid beta protein induces oxidative damage of mitochondrial DNA. J Neuropathol Exp Neurol. 1997; 56(12):1356-62. DOI: 10.1097/00005072-199712000-00010. View

13.

Glenner G, Wong C . Alzheimer's disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein. Biochem Biophys Res Commun. 1984; 120(3):885-90. DOI: 10.1016/s0006-291x(84)80190-4. View

14.

Lu X, HongCai S, Jiaying W, Jing H, Jun X . Assessing the quality of reports about randomized controlled trials of acupuncture treatment on mild cognitive impairment. PLoS One. 2011; 6(2):e16922. PMC: 3045380. DOI: 10.1371/journal.pone.0016922. View

15.

Shen P, Kong L, Ni L, Guo H, Yang S, Zhang L . Acupuncture intervention in ischemic stroke: a randomized controlled prospective study. Am J Chin Med. 2012; 40(4):685-93. DOI: 10.1142/S0192415X12500516. View

16.

Kim S, Lee B, Bae J, Kim K, Steffensen S, Ryu Y . Peripheral afferent mechanisms underlying acupuncture inhibition of cocaine behavioral effects in rats. PLoS One. 2013; 8(11):e81018. PMC: 3832370. DOI: 10.1371/journal.pone.0081018. View

17.

Manczak M, Anekonda T, Henson E, Park B, Quinn J, Reddy P . Mitochondria are a direct site of A beta accumulation in Alzheimer's disease neurons: implications for free radical generation and oxidative damage in disease progression. Hum Mol Genet. 2006; 15(9):1437-49. DOI: 10.1093/hmg/ddl066. View

18.

Aggarwal N, Tripathi M, Dodge H, Alladi S, Anstey K . Trends in Alzheimer's disease and dementia in the asian-pacific region. Int J Alzheimers Dis. 2013; 2012:171327. PMC: 3523465. DOI: 10.1155/2012/171327. View

19.

Wang J, Markesbery W, Lovell M . Increased oxidative damage in nuclear and mitochondrial DNA in mild cognitive impairment. J Neurochem. 2006; 96(3):825-32. DOI: 10.1111/j.1471-4159.2005.03615.x. View

20.

McBride H, Neuspiel M, Wasiak S . Mitochondria: more than just a powerhouse. Curr Biol. 2006; 16(14):R551-60. DOI: 10.1016/j.cub.2006.06.054. View