Complex Reproductive Secretions Occur in All Extant Gymnosperm Lineages: a Proteomic Survey of Gymnosperm Pollination Drops

Overview

Journal Plant Reprod

Publisher Springer

Specialty Biology

Date 2018 Nov 16

PMID 30430247

Citations 7

Authors

Natalie Prior

Stefan A Little

Ian Boyes

Patrick Griffith

Chad Husby

Cary Pirone-Davies

Dennis W Stevenson

P Barry Tomlinson

Patrick von Aderkas

Affiliations

Soon will be listed here.

Abstract

Complex protein-containing reproductive secretions are a conserved trait amongst all extant gymnosperms; the pollination drops of most groups include carbohydrate-modifying enzymes and defence proteins. Pollination drops are aqueous secretions that receive pollen and transport it to the ovule interior in gymnosperms (Coniferales, Cycadales, Ginkgoales, Gnetales). Proteins are well established as components of pollination drops in conifers (Coniferales) and Ephedra spp. (Gnetales), but it is unknown whether proteins are also present in the pollination drops of cycads (Cycadales), Ginkgo (Ginkgoales), Gnetum (Gnetales), or in the pollination drops produced by sterile ovules occurring on pollen plants in the Gnetales. We used liquid chromatography-tandem mass spectrometry followed by database-derived protein identification to conduct proteomic surveys of pollination drops collected from: Ceratozamia hildae, Zamia furfuracea and Cycas rumphii (Cycadales); Ginkgo biloba (Ginkgoales); Gnetum gnemon and Welwitschia mirabilis, including pollination drops from both microsporangiate and ovulate plants (Gnetales). We identified proteins in all samples: C. hildae (61), Z. furfuracea (40), C. rumphii (9), G. biloba (57), G. gnemon ovulate (17) and sterile ovules from microsporangiate plants (25) and W. mirabilis fertile ovules (1) and sterile ovules from microsporangiate plants (138). Proteins involved in defence and carbohydrate modification occurred in the drops of most groups, indicating conserved functions for proteins in pollination drops. Our study demonstrates that all extant gymnosperm groups produce complex reproductive secretions containing proteins, an ancient trait that likely contributed to the evolutionary success of seed plants.

Citing Articles

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Teyssier C, Rogier O, Claverol S, Gautier F, Lelu-Walter M, Durufle H Biomolecules. 2023; 13(9).

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Composition of Sexual Fluids in Ovules During Pollination and Fertilization.

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