Effects of Probiotic Supplementation on Selected Parameters of Blood Prooxidant-Antioxidant Balance in Elite Athletes: A Double-Blind Randomized Placebo-Controlled Study

Overview

Journal J Hum Kinet

Publisher Termedia

Date 2018 Nov 16

PMID 30429904

Citations 15

Authors

Danica Michalickova

Jelena Kotur-Stevuljevic

Milica Miljkovic

Nenad Dikic

Marija Kostic-Vucicevic

Marija Andjelkovic

Vladimir Koricanac

Brizita Djordjevic

Affiliations

Soon will be listed here.

Abstract

A randomized, double-blind, placebo-controlled study was conducted, in order to evaluate if Lactobacillus helveticus Lafti® L10 (Lallemand Health Solutions, Montreal, Canada) supplementation during three months could influence oxidative markers in the population of elite athletes: triathletes, cyclists and endurance athletes. Twenty-two elite athletes were randomized to either placebo (n = 12) or probiotic (n = 10) groups. The probiotic group received 2x1010 colony forming units of Lafti® L10. Before and after the supplementation serum samples were collected. Markers of oxidative stress and anti-oxidative defense: superoxide dismutase (SOD), paraoxonase (PON), advanced oxidation protein products (AOPP), malondialdehyde (MDA), total antioxidant status, total oxidant status, pro-oxidant-antioxidant balance, oxidative stress index, bilirubin, uric acid and albumin were determined in serum. Parameters of lipid status, as well as susceptibility to copper-induced oxidation of LDL particles in vitro were also determined. There was a significant interaction effect for MDA (p = 0.039), with a decrease in MDA in the probiotic group only (p = 0.049). There was a significant interaction effect for AOPP (p = 0.037), with a significant decrease in the probiotic group (p = 0.045). Interaction effect for SOD was approaching to formal significance (p = 0.108) and the post-hoc test showed a significant decrease in the probiotic group (p = 0.041) only. A significant correlation between AOPP and SOD (p = 0.012, r = -0.40) was found in the probiotic group at the end of the study. PON1 activity was decreased in both the probiotic (p = 0.032) and placebo group (p = 0.035). No significant changes in the remainder of the evaluated parameters were noted. In conclusion, probiotic strain Lafti® L10 exerts certain antioxidant potential, but further research is needed.

Citing Articles

The Effect of Synbiotic Supplementation on Bone Complications, Anemia, and Gastrointestinal Function in Hemodialysis Patients: A Double-Blind Randomized Clinical Trial.

Azamian Y, Abdollahzad H, Rezaeian S, Rouhani M, Fatehi M Clin Nutr Res. 2024; 13(4):272-283.

PMID: 39526206 PMC: 11543452. DOI: 10.7762/cnr.2024.13.4.272.

Effects of probiotic supplementation on 12 min run performance, mood management, body composition and gut microbiota in amateur marathon runners: A double-blind controlled trial.

Wang L, Meng F, Jin Y, Wu L, Tang R, Xu K J Exerc Sci Fit. 2024; 22(4):297-304.

PMID: 38706951 PMC: 11066675. DOI: 10.1016/j.jesf.2024.04.004.

Lactiplantibacillus plantarum strains KABP011, KABP012, and KABP013 modulate bile acids and cholesterol metabolism in humans.

Padro T, Santisteban V, Huedo P, Puntes M, Aguilo M, Espadaler-Mazo J Cardiovasc Res. 2024; 120(7):708-722.

PMID: 38525555 PMC: 11135648. DOI: 10.1093/cvr/cvae061.

How Supplementation with SOD-Rich Plant Extract, Combined with Gliadin, Can Affect Oxidative Stress Markers and Zonulin Levels in Exercise-Induced Oxidative Stress.

Dudasova Petrovicova O, Stankovic I, ordevic B, Dopsaj V, Milinkovic N, Dopsaj M Metabolites. 2023; 13(12).

PMID: 38132882 PMC: 10745368. DOI: 10.3390/metabo13121200.

Neuroprotective Effects of Sinomenine on Experimental Autoimmune Encephalomyelitis via Anti-Inflammatory and Nrf2-Dependent Anti-Oxidative Stress Activity.

Fan H, Yang Y, Bai Q, Wang D, Shi X, Zhang L Neuromolecular Med. 2023; 25(4):545-562.

PMID: 37735290 DOI: 10.1007/s12017-023-08756-z.

References

Michalickova D, Kostic-Vucicevic M, Vukasinovic-Vesic M, Stojmenovic T, Dikic N, Andjelkovic M . Lactobacillus helveticus Lafti L10 Supplementation Modulates Mucosal and Humoral Immunity in Elite Athletes: A Randomized, Double-Blind, Placebo-Controlled Trial. J Strength Cond Res. 2016; 31(1):62-70. DOI: 10.1519/JSC.0000000000001456. View

Senti M, Tomas M, Anglada R, Elosua R, Marrugat J, Covas M . Interrelationship of smoking, paraoxonase activity, and leisure time physical activity: a population-based study. Eur J Intern Med. 2003; 14(3):178-184. DOI: 10.1016/s0953-6205(03)00041-4. View

Gleeson M, Bishop N, Oliveira M, Tauler P . Influence of training load on upper respiratory tract infection incidence and antigen-stimulated cytokine production. Scand J Med Sci Sports. 2011; 23(4):451-7. DOI: 10.1111/j.1600-0838.2011.01422.x. View

Alamdari D, Paletas K, Pegiou T, Sarigianni M, Befani C, Koliakos G . A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients. Clin Biochem. 2007; 40(3-4):248-54. DOI: 10.1016/j.clinbiochem.2006.10.017. View

Melville G, Siegler J, Marshall P . The effects of d-aspartic acid supplementation in resistance-trained men over a three month training period: A randomised controlled trial. PLoS One. 2017; 12(8):e0182630. PMC: 5571970. DOI: 10.1371/journal.pone.0182630. View

Girotti M, Khan N, McLellan B . Early measurement of systemic lipid peroxidation products in the plasma of major blunt trauma patients. J Trauma. 1991; 31(1):32-5. DOI: 10.1097/00005373-199101000-00007. View

Mishra V, Shah C, Mokashe N, Chavan R, Yadav H, Prajapati J . Probiotics as potential antioxidants: a systematic review. J Agric Food Chem. 2015; 63(14):3615-26. DOI: 10.1021/jf506326t. View

Erel O . A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004; 37(4):277-85. DOI: 10.1016/j.clinbiochem.2003.11.015. View

Michalickova D, Minic R, Dikic N, Andjelkovic M, Kostic-Vucicevic M, Stojmenovic T . Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial. Appl Physiol Nutr Metab. 2016; 41(7):782-9. DOI: 10.1139/apnm-2015-0541. View

10.

Erel O . A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38(12):1103-11. DOI: 10.1016/j.clinbiochem.2005.08.008. View

11.

Witko-Sarsat V, Friedlander M, Nguyen-Khoa T, Nguyen A, Zingraff J, Jungers P . Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int. 1996; 49(5):1304-13. DOI: 10.1038/ki.1996.186. View

12.

Lamprecht M, Bogner S, Schippinger G, Steinbauer K, Fankhauser F, Hallstroem S . Probiotic supplementation affects markers of intestinal barrier, oxidation, and inflammation in trained men; a randomized, double-blinded, placebo-controlled trial. J Int Soc Sports Nutr. 2012; 9(1):45. PMC: 3465223. DOI: 10.1186/1550-2783-9-45. View

13.

Martarelli D, Verdenelli M, Scuri S, Cocchioni M, Silvi S, Cecchini C . Effect of a probiotic intake on oxidant and antioxidant parameters in plasma of athletes during intense exercise training. Curr Microbiol. 2011; 62(6):1689-96. DOI: 10.1007/s00284-011-9915-3. View

14.

Richter R, Furlong C . Determination of paraoxonase (PON1) status requires more than genotyping. Pharmacogenetics. 2000; 9(6):745-53. View

15.

Martinovic J, Dopsaj V, Dopsaj M, Kotur-Stevuljevic J, Vujovic A, Stefanovic A . Long-term effects of oxidative stress in volleyball players. Int J Sports Med. 2009; 30(12):851-6. DOI: 10.1055/s-0029-1238289. View

16.

Ejtahed H, Mohtadi-Nia J, Homayouni-Rad A, Niafar M, Asghari-Jafarabadi M, Mofid V . Probiotic yogurt improves antioxidant status in type 2 diabetic patients. Nutrition. 2011; 28(5):539-43. DOI: 10.1016/j.nut.2011.08.013. View

17.

Teixeira V, Valente H, Casal S, Marques A, Moreira P . Antioxidants do not prevent postexercise peroxidation and may delay muscle recovery. Med Sci Sports Exerc. 2009; 41(9):1752-60. DOI: 10.1249/MSS.0b013e31819fe8e3. View

18.

Ainsworth B, Haskell W, Herrmann S, Meckes N, Bassett Jr D, Tudor-Locke C . 2011 Compendium of Physical Activities: a second update of codes and MET values. Med Sci Sports Exerc. 2011; 43(8):1575-81. DOI: 10.1249/MSS.0b013e31821ece12. View

19.

Tomas M, Elosua R, Senti M, Molina L, Vila J, Anglada R . Paraoxonase1-192 polymorphism modulates the effects of regular and acute exercise on paraoxonase1 activity. J Lipid Res. 2002; 43(5):713-20. View

20.

Misra H, Fridovich I . The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972; 247(10):3170-5. View