Amino Acid Composition of Nanofibrillar Self-assembling Peptide Hydrogels Affects Responses of Periodontal Tissue Cells in Vitro

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2018 Nov 15

PMID 30425485

Citations 6

Authors

Franziska Koch

Anne Wolff

Stephanie Mathes

Uwe Pieles

Sina S Saxer

Bernd Kreikemeyer

Kirsten Peters

Affiliations

Soon will be listed here.

Abstract

Background: The regeneration of tissue defects at the interface between soft and hard tissue, eg, in the periodontium, poses a challenge due to the divergent tissue requirements. A class of biomaterials that may support the regeneration at the soft-to-hard tissue interface are self-assembling peptides (SAPs), as their physicochemical and mechanical properties can be rationally designed to meet tissue requirements.

Materials And Methods: In this work, we investigated the effect of two single-component and two complementary β-sheet forming SAP systems on their hydrogel properties such as nanofibrillar architecture, surface charge, and protein adsorption as well as their influence on cell adhesion, morphology, growth, and differentiation.

Results: We showed that these four 11-amino acid SAP (P11-SAP) hydrogels possessed physico-chemical characteristics dependent on their amino acid composition that allowed variabilities in nanofibrillar network architecture, surface charge, and protein adsorption (eg, the single-component systems demonstrated an ~30% higher porosity and an almost 2-fold higher protein adsorption compared with the complementary systems). Cytocompatibility studies revealed similar results for cells cultured on the four P11-SAP hydrogels compared with cells on standard cell culture surfaces. The single-component P11-SAP systems showed a 1.7-fold increase in cell adhesion and cellular growth compared with the complementary P11-SAP systems. Moreover, significantly enhanced osteogenic differentiation of human calvarial osteoblasts was detected for the single-component P11-SAP system hydrogels compared with standard cell cultures.

Conclusion: Thus, single-component system P11-SAP hydrogels can be assessed as suitable scaffolds for periodontal regeneration therapy, as they provide adjustable, extracellular matrix-mimetic nanofibrillar architecture and favorable cellular interaction with periodontal cells.

Citing Articles

New Insights in Hydrogels for Periodontal Regeneration.

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Yazdanian M, Alam M, Abbasi K, Rahbar M, Farjood A, Tahmasebi E Front Bioeng Biotechnol. 2022; 10:987195.

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[Research progress on the effect of surface charge of biomaterials on bone formation].

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Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges.

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PMID: 33015006 PMC: 7505933. DOI: 10.3389/fbioe.2020.01016.

Development and application of a 3D periodontal in vitro model for the evaluation of fibrillar biomaterials.

Koch F, Meyer N, Valdec S, Jung R, Mathes S BMC Oral Health. 2020; 20(1):148.

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