Elevated MicroRNA-141-3p in Placenta of Non-diabetic Macrosomia Regulate Trophoblast Proliferation

Overview

Journal EBioMedicine

Specialty Biomedical Engineering

Date 2018 Nov 14

PMID 30420300

Citations 7

Authors

Dan Guo

Hua Jiang

Yiqiu Chen

Jing Yang

Ziqiang Fu

Jing Li

Xiumei Han

Xian Wu

Yankai Xia

Xinru Wang

Liping Chen

Qiuqin Tang

Wei Wu

Affiliations

Soon will be listed here.

Abstract

Background: Several studies have reported microRNAs (miRNAs) could regulate the placental development, though the role and mechanism of miRNAs in the development of non-diabetic macrosomia (NDFMS) remains unclear.

Methods: To identify the aberrantly expressed key miRNAs in placenta of NDFMS, we employed a strategy consisting of initial screening with miRNA microarray and further validation with quantitative RT-PCR assay (qRT-PCR). In vitro cellular model and a mouse pregnancy model were used to delineate the functional effects of key miRNA on proliferation, invasion, and migration.

Findings: miR-141-3p was identified as the key miRNA with expression level significantly higher in placentas of NDFMS compared with those from normal controls. Overexpressed miR-141-3p in HTR-8/SVneo cells contributed to increased cell proliferation, invasion, and migration. miR-141-3p inhibition in HTR-8/SVneo cells resulted in decreased cell proliferation and invasion. Significantly increased infant birth weight was observed in late pregnancy of C57BL/6J mice treated with miR-141-3p agomir. However, no significant difference was found in early pregnancy of C57BL/6J mice treated with miR-141-3p agomir.

Interpretation: miR-141-3p could stimulate placental cell proliferation to participate in the occurrence and development of NDFMS.

Citing Articles

The role of microRNAs in pregnancies complicated by maternal diabetes.

Owen M, Kennedy M, Quilang R, Scott E, Forbes K Clin Sci (Lond). 2024; 138(18):1179-1207.

PMID: 39289953 PMC: 11409017. DOI: 10.1042/CS20230681.

The Role of Placental Non-Coding RNAs in Adverse Pregnancy Outcomes.

Ren J, Jin H, Zhu Y Int J Mol Sci. 2023; 24(5).

PMID: 36902459 PMC: 10003511. DOI: 10.3390/ijms24055030.

Embryo cryopreservation leads to sex-specific DNA methylation perturbations in both human and mouse placentas.

Mani S, Ghosh J, Rhon-Calderon E, Lan Y, Ord T, Kalliora C Hum Mol Genet. 2022; 31(22):3855-3872.

PMID: 35717573 PMC: 9652110. DOI: 10.1093/hmg/ddac138.

Downregulation of lncRNA in the placentas of pregnant women with non‑diabetic fetal macrosomia promotes trophoblast cell proliferation.

Lu Y, Tang Q, Yang S, Cheng Y, Li M, Guo D Mol Med Rep. 2022; 26(2).

PMID: 35674181 PMC: 9218729. DOI: 10.3892/mmr.2022.12766.

MiR-141-3p regulates myogenic differentiation in C2C12 myoblasts via CFL2-YAP-mediated mechanotransduction.

Nguyen M, Lee W BMB Rep. 2022; 55(2):104-109.

PMID: 35000671 PMC: 8891624.

References

Higashijima A, Miura K, Mishima H, Kinoshita A, Jo O, Abe S . Characterization of placenta-specific microRNAs in fetal growth restriction pregnancy. Prenat Diagn. 2013; 33(3):214-22. DOI: 10.1002/pd.4045. View

Longtine M, Nelson D . Placental dysfunction and fetal programming: the importance of placental size, shape, histopathology, and molecular composition. Semin Reprod Med. 2011; 29(3):187-96. PMC: 3682832. DOI: 10.1055/s-0031-1275515. View

Jimenez P, Mainigi M, Word R, Kraus W, Mendelson C . miR-200 Regulates Endometrial Development During Early Pregnancy. Mol Endocrinol. 2016; 30(9):977-87. PMC: 5004115. DOI: 10.1210/me.2016-1050. View

Li J, Zhang Z, Du M, Guan Y, Yao J, Yu H . microRNA-141-3p fosters the growth, invasion, and tumorigenesis of cervical cancer cells by targeting FOXA2. Arch Biochem Biophys. 2018; 657:23-30. DOI: 10.1016/j.abb.2018.09.008. View

Koyanagi A, Zhang J, Dagvadorj A, Hirayama F, Shibuya K, Souza J . Macrosomia in 23 developing countries: an analysis of a multicountry, facility-based, cross-sectional survey. Lancet. 2013; 381(9865):476-83. DOI: 10.1016/S0140-6736(12)61605-5. View

Li J, Chen L, Tang Q, Wu W, Gu H, Liu L . The role, mechanism and potentially novel biomarker of microRNA-17-92 cluster in macrosomia. Sci Rep. 2015; 5:17212. PMC: 4657041. DOI: 10.1038/srep17212. View

Sabri A, Lai D, DSilva A, Seeho S, Kaur J, Ng C . Differential placental gene expression in term pregnancies affected by fetal growth restriction and macrosomia. Fetal Diagn Ther. 2014; 36(2):173-80. DOI: 10.1159/000360535. View

Li J, Fu Z, Jiang H, Chen L, Wu X, Ding H . IGF2-derived miR-483-3p contributes to macrosomia through regulating trophoblast proliferation by targeting RB1CC1. Mol Hum Reprod. 2018; 24(9):444-452. DOI: 10.1093/molehr/gay027. View

Tian F, Wang X, Xie C, Zhao B, Niu Z, Fan L . Placental surface area mediates the association between methylation in placenta and full-term low birth weight in girls. Clin Epigenetics. 2018; 10:39. PMC: 5863829. DOI: 10.1186/s13148-018-0472-5. View

10.

Pundir J, Sinha P . Non-diabetic macrosomia: an obstetric dilemma. J Obstet Gynaecol. 2009; 29(3):200-5. DOI: 10.1080/01443610902735140. View

11.

Shi P, Yang W, Yu Q, Zhao Q, Li C, Ma X . Overweight, gestational weight gain and elevated fasting plasma glucose and their association with macrosomia in chinese pregnant women. Matern Child Health J. 2013; 18(1):10-15. DOI: 10.1007/s10995-013-1253-6. View

12.

Winterbottom E, Fei D, Koestler D, Giambelli C, Wika E, Capobianco A . GLI3 Links Environmental Arsenic Exposure and Human Fetal Growth. EBioMedicine. 2015; 2(6):536-43. PMC: 4535308. DOI: 10.1016/j.ebiom.2015.04.019. View

13.

Chim S, Shing T, Hung E, Leung T, Lau T, Chiu R . Detection and characterization of placental microRNAs in maternal plasma. Clin Chem. 2008; 54(3):482-90. DOI: 10.1373/clinchem.2007.097972. View

14.

Mak C, Yung M, Hui L, Leung L, Liang R, Chen K . MicroRNA-141 enhances anoikis resistance in metastatic progression of ovarian cancer through targeting KLF12/Sp1/survivin axis. Mol Cancer. 2017; 16(1):11. PMC: 5240442. DOI: 10.1186/s12943-017-0582-2. View

15.

Ospina-Prieto S, Chaiwangyen W, Herrmann J, Groten T, Schleussner E, Markert U . MicroRNA-141 is upregulated in preeclamptic placentae and regulates trophoblast invasion and intercellular communication. Transl Res. 2016; 172:61-72. DOI: 10.1016/j.trsl.2016.02.012. View

16.

Tang Q, Wu W, Xu X, Huang L, Gao Q, Chen H . miR-141 contributes to fetal growth restriction by regulating PLAG1 expression. PLoS One. 2013; 8(3):e58737. PMC: 3598866. DOI: 10.1371/journal.pone.0058737. View

17.

Murphy M, Casselman R, Tayade C, Smith G . Differential expression of plasma microRNA in preeclamptic patients at delivery and 1 year postpartum. Am J Obstet Gynecol. 2015; 213(3):367.e1-9. DOI: 10.1016/j.ajog.2015.05.013. View

18.

Rossant J, CROSS J . Placental development: lessons from mouse mutants. Nat Rev Genet. 2001; 2(7):538-48. DOI: 10.1038/35080570. View

19.

Lou G, Dong X, Xia C, Ye B, Yan Q, Wu S . Direct targeting sperm-associated antigen 9 by miR-141 influences hepatocellular carcinoma cell growth and metastasis via JNK pathway. J Exp Clin Cancer Res. 2016; 35:14. PMC: 4721207. DOI: 10.1186/s13046-016-0289-z. View

20.

Yuan H, Sun K, Yu K . Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway. Mol Med Rep. 2013; 9(1):350-4. DOI: 10.3892/mmr.2013.1786. View