Detection of Copro-prevalence by Polymerase Chain Reaction Using Repetitive 529 Bp Gene in Feces of Pet Cats () in Yogyakarta, Indonesia

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2018 Nov 10

PMID 30410243

Citations 3

Authors

Muhammad Hanafiah

Joko Prastowo

Sri Hartati

Dwinna Aliza

Raden Wisnu Nurcahyo

Affiliations

Soon will be listed here.

Abstract

Aim: The aim of this research was to determine the copro-prevalence of using polymerase chain reaction (PCR) with repetitive 529 bp gene and to construct the phylogenetic tree of oocyst from pet cats in Yogyakarta.

Materials And Methods: 9 of 132 pet cat samples which serologically positive for were used in this research. To determine the copro-prevalence of in pet cat, 10 g of feces samples taken from practitioners and household cats in Yogyakarta were used in the PCR method utilizing repetitive 529 bp gene sequences.

Results: The result shows that copro-prevalence by PCR using repetitive 529 bp gene was 33.3% (3/9). The phylogenetic tree of grouped into two clades, which clade 1 consists of isolates collected from pet cats in Yogyakarta Indonesia and isolates from China and in clade 2 consist of the isolates from India.

Conclusion: Copro-prevalence of in pet cats in Yogyakarta by means of PCR using repetitive 529 bp gene is around 33.3%.

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