Functional Analysis of a Putative Target of Spatially Varying Selection in the Gene of

Overview

Journal G3 (Bethesda)

Publisher Oxford University Press

Specialties Genetics
Molecular Biology

Date 2018 Nov 9

PMID 30404774

Citations 4

Authors

Nicolas Svetec

Perot Saelao

Julie M Cridland

Ary A Hoffmann

David J Begun

Affiliations

Soon will be listed here.

Abstract

While significant effort has been devoted to investigating the potential influence of spatially varying selection on genomic variation, relatively little effort has been devoted to experimental analysis of putative variants or genes experiencing such selection. Previous population genetic work identified an amino acid polymorphism in the gene as one of the most strongly latitudinally differentiated SNPs in the genome of in the United States and Australia. Here we report the results of our transgenic analysis of this amino acid polymorphism. Genotypes carrying alternative alleles differed in multiple phenotypes in a direction generally consistent with phenotypic differences previously observed along latitudinal clines. These results support inferences from earlier population genomic work that this variant influences fitness, and support the idea that the alleles exhibiting clines may be likely to have pleiotropic effects that are correlated along the axes favored by natural selection.

Citing Articles

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A clinal polymorphism in the insulin signaling transcription factor foxo contributes to life-history adaptation in Drosophila.

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References

Papaconstantinou M, Pepper A, Wu Y, Kasimer D, Westwood T, Campos A . Menin links the stress response to genome stability in Drosophila melanogaster. PLoS One. 2010; 5(11):e14049. PMC: 2987805. DOI: 10.1371/journal.pone.0014049. View

Yeaman S . Local Adaptation by Alleles of Small Effect. Am Nat. 2015; 186 Suppl 1:S74-89. DOI: 10.1086/682405. View

Chan Y, Marks M, Jones F, Villarreal Jr G, Shapiro M, Brady S . Adaptive evolution of pelvic reduction in sticklebacks by recurrent deletion of a Pitx1 enhancer. Science. 2009; 327(5963):302-5. PMC: 3109066. DOI: 10.1126/science.1182213. View

Copeland N, Jenkins N, Court D . Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet. 2001; 2(10):769-79. DOI: 10.1038/35093556. View

Catalan A, Glaser-Schmitt A, Argyridou E, Duchen P, Parsch J . An Indel Polymorphism in the MtnA 3' Untranslated Region Is Associated with Gene Expression Variation and Local Adaptation in Drosophila melanogaster. PLoS Genet. 2016; 12(4):e1005987. PMC: 4847869. DOI: 10.1371/journal.pgen.1005987. View

Blom N, Gammeltoft S, Brunak S . Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. J Mol Biol. 1999; 294(5):1351-62. DOI: 10.1006/jmbi.1999.3310. View

Bozicevic V, Hutter S, Stephan W, Wollstein A . Population genetic evidence for cold adaptation in European Drosophila melanogaster populations. Mol Ecol. 2015; 25(5):1175-91. DOI: 10.1111/mec.13464. View

Adrion J, Hahn M, Cooper B . Revisiting classic clines in Drosophila melanogaster in the age of genomics. Trends Genet. 2015; 31(8):434-44. PMC: 4526433. DOI: 10.1016/j.tig.2015.05.006. View

Simmons M, Crow J . Mutations affecting fitness in Drosophila populations. Annu Rev Genet. 1977; 11:49-78. DOI: 10.1146/annurev.ge.11.120177.000405. View

10.

Schmidt P, Zhu C, Das J, Batavia M, Yang L, Eanes W . An amino acid polymorphism in the couch potato gene forms the basis for climatic adaptation in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2008; 105(42):16207-11. PMC: 2570987. DOI: 10.1073/pnas.0805485105. View

11.

Reinhardt J, Kolaczkowski B, Jones C, Begun D, Kern A . Parallel geographic variation in Drosophila melanogaster. Genetics. 2014; 197(1):361-73. PMC: 4012493. DOI: 10.1534/genetics.114.161463. View

12.

Yang Y, Edery I . Parallel clinal variation in the mid-day siesta of Drosophila melanogaster implicates continent-specific targets of natural selection. PLoS Genet. 2018; 14(9):e1007612. PMC: 6138418. DOI: 10.1371/journal.pgen.1007612. View

13.

Turner T, Levine M, Eckert M, Begun D . Genomic analysis of adaptive differentiation in Drosophila melanogaster. Genetics. 2008; 179(1):455-73. PMC: 2390623. DOI: 10.1534/genetics.107.083659. View

14.

Lack J, Cardeno C, Crepeau M, Taylor W, Corbett-Detig R, Stevens K . The Drosophila genome nexus: a population genomic resource of 623 Drosophila melanogaster genomes, including 197 from a single ancestral range population. Genetics. 2015; 199(4):1229-41. PMC: 4391556. DOI: 10.1534/genetics.115.174664. View

15.

Kolaczkowski B, Kern A, Holloway A, Begun D . Genomic differentiation between temperate and tropical Australian populations of Drosophila melanogaster. Genetics. 2010; 187(1):245-60. PMC: 3018305. DOI: 10.1534/genetics.110.123059. View

16.

Laurie-Ahlberg C, Stam L . Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster. Genetics. 1987; 115(1):129-40. PMC: 1203048. DOI: 10.1093/genetics/115.1.129. View

17.

Chappell M, Hayes J, Snyder L . HEMOGLOBIN POLYMORPHISMS IN DEER MICE (PEROMYSCUS MANICULATUS): PHYSIOLOGY OF BETA-GLOBIN VARIANTS AND ALPHA-GLOBIN RECOMBINANTS. Evolution. 2017; 42(4):681-688. DOI: 10.1111/j.1558-5646.1988.tb02486.x. View

18.

Machado H, Bergland A, OBrien K, Behrman E, Schmidt P, Petrov D . Comparative population genomics of latitudinal variation in Drosophila simulans and Drosophila melanogaster. Mol Ecol. 2015; 25(3):723-40. PMC: 5089931. DOI: 10.1111/mec.13446. View

19.

Svetec N, Werzner A, Wilches R, Pavlidis P, Alvarez-Castro J, Broman K . Identification of X-linked quantitative trait loci affecting cold tolerance in Drosophila melanogaster and fine mapping by selective sweep analysis. Mol Ecol. 2011; 20(3):530-44. PMC: 3668310. DOI: 10.1111/j.1365-294X.2010.04951.x. View

20.

Hilbish T, Koehn R . THE PHYSIOLOGICAL BASIS OF NATURAL SELECTION AT THE LAP LOCUS. Evolution. 2017; 39(6):1302-1317. DOI: 10.1111/j.1558-5646.1985.tb05696.x. View