MicroR408 Regulates Defense Response Upon Wounding in Sweet Potato

Overview

Journal J Exp Bot

Publisher Oxford University Press

Specialty Biology

Date 2018 Nov 8

PMID 30403812

Citations 17

Authors

Yun-Wei Kuo

Jeng-Shane Lin

Yu-Chi Li

Min-Yao Jhu

Yu-Chi King

Shih-Tong Jeng

Affiliations

Soon will be listed here.

Abstract

MiRNAs play diverse roles in plant development and defense responses by binding to their mRNA targets based on sequence complementarity. Here, we investigated a wound-related miR408 and its target genes in sweet potato (Ipomoea batatas) by small RNA deep sequencing and transcriptome analysis. The expression patterns of miR408 and the miR408 precursor were significantly repressed by wounding and jasmonate (JA). In contrast, expression of the putative target genes IbKCS (3-ketoacyl-CoA synthase 4), IbPCL (plantacyanin), and IbGAUT (galacturonosyltransferase 7-like) of miR408 was increased following wounding, whereas only IbKCS was increased after JA treatment. Target cleavage site mapping and Agrobacterium-mediated transient assay demonstrated that IbKCS, IbPCL, and IbGAUT were the targets of miR408. The expression of miR408 target genes was repressed in transgenic sweet potatoes overexpressing miR408. These data indicated a relationship between miR408 and its target genes. Notably, miR408-overexpressing plants showed a semi-dwarf phenotype and attenuated resistance to insect feeding, while transgenic plants overexpressing IbKCS exhibited more insect resistance than plants overexpressing only the empty vector. Collectively, sweet potato reduces the abundance of miR408 upon wounding to elevate the expression of IbKCS, IbPCL, and IbGAUT. The expression of IbKCS enhances the defense system against herbivore wounding.

Citing Articles

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References

Bozorov T, Baldwin I, Kim S . Identification and profiling of miRNAs during herbivory reveals jasmonate-dependent and -independent patterns of accumulation in Nicotiana attenuata. BMC Plant Biol. 2012; 12:209. PMC: 3502350. DOI: 10.1186/1471-2229-12-209. View

Starega-Roslan J, Krol J, Koscianska E, Kozlowski P, Szlachcic W, Sobczak K . Structural basis of microRNA length variety. Nucleic Acids Res. 2010; 39(1):257-68. PMC: 3017592. DOI: 10.1093/nar/gkq727. View

Axtell M, Westholm J, Lai E . Vive la différence: biogenesis and evolution of microRNAs in plants and animals. Genome Biol. 2011; 12(4):221. PMC: 3218855. DOI: 10.1186/gb-2011-12-4-221. View

Rajendran S, Lin I, Chen M, Chen C, Yeh K . Differential activation of sporamin expression in response to abiotic mechanical wounding and biotic herbivore attack in the sweet potato. BMC Plant Biol. 2014; 14:112. PMC: 4108030. DOI: 10.1186/1471-2229-14-112. View

Feng H, Zhang Q, Wang Q, Wang X, Liu J, Li M . Target of tae-miR408, a chemocyanin-like protein gene (TaCLP1), plays positive roles in wheat response to high-salinity, heavy cupric stress and stripe rust. Plant Mol Biol. 2013; 83(4-5):433-43. DOI: 10.1007/s11103-013-0101-9. View

Ruan X, Luo F, Li D, Zhang J, Liu Z, Xu W . Cotton BCP genes encoding putative blue copper-binding proteins are functionally expressed in fiber development and involved in response to high-salinity and heavy metal stresses. Physiol Plant. 2010; 141(1):71-83. DOI: 10.1111/j.1399-3054.2010.01420.x. View

Weidenbach D, Jansen M, Franke R, Hensel G, Weissgerber W, Ulferts S . Evolutionary conserved function of barley and Arabidopsis 3-KETOACYL-CoA SYNTHASES in providing wax signals for germination of powdery mildew fungi. Plant Physiol. 2014; 166(3):1621-33. PMC: 4226380. DOI: 10.1104/pp.114.246348. View

Meyers B, Axtell M, Bartel B, Bartel D, Baulcombe D, Bowman J . Criteria for annotation of plant MicroRNAs. Plant Cell. 2008; 20(12):3186-90. PMC: 2630443. DOI: 10.1105/tpc.108.064311. View

Pozo M, van der Ent S, van Loon L, Pieterse C . Transcription factor MYC2 is involved in priming for enhanced defense during rhizobacteria-induced systemic resistance in Arabidopsis thaliana. New Phytol. 2008; 180(2):511-523. DOI: 10.1111/j.1469-8137.2008.02578.x. View

10.

Mitchell C, Brennan R, Graham J, Karley A . Plant Defense against Herbivorous Pests: Exploiting Resistance and Tolerance Traits for Sustainable Crop Protection. Front Plant Sci. 2016; 7:1132. PMC: 4965446. DOI: 10.3389/fpls.2016.01132. View

11.

Zhao X, Hong P, Wu J, Chen X, Ye X, Pan Y . The tae-miR408-Mediated Control of TaTOC1 Genes Transcription Is Required for the Regulation of Heading Time in Wheat. Plant Physiol. 2016; 170(3):1578-94. PMC: 4775108. DOI: 10.1104/pp.15.01216. View

12.

Gorb E, Gorb S . Anti-adhesive effects of plant wax coverage on insect attachment. J Exp Bot. 2017; 68(19):5323-5337. DOI: 10.1093/jxb/erx271. View

13.

Miskiewicz J, Tomczyk K, Mickiewicz A, Sarzynska J, Szachniuk M . Bioinformatics Study of Structural Patterns in Plant MicroRNA Precursors. Biomed Res Int. 2017; 2017:6783010. PMC: 5322449. DOI: 10.1155/2017/6783010. View

14.

. A simple and general method for transferring genes into plants. Science. 1985; 227(4691):1229-31. DOI: 10.1126/science.227.4691.1229. View

15.

De Bigault Du Granrut A, Cacas J . How Very-Long-Chain Fatty Acids Could Signal Stressful Conditions in Plants?. Front Plant Sci. 2016; 7:1490. PMC: 5067520. DOI: 10.3389/fpls.2016.01490. View

16.

Zhang J, Yu Y, Feng Y, Zhou Y, Zhang F, Yang Y . MiR408 Regulates Grain Yield and Photosynthesis via a Phytocyanin Protein. Plant Physiol. 2017; 175(3):1175-1185. PMC: 5664482. DOI: 10.1104/pp.17.01169. View

17.

Orfila C, Sorensen S, Harholt J, Geshi N, Crombie H, Truong H . QUASIMODO1 is expressed in vascular tissue of Arabidopsis thaliana inflorescence stems, and affects homogalacturonan and xylan biosynthesis. Planta. 2005; 222(4):613-22. DOI: 10.1007/s00425-005-0008-z. View

18.

Voinnet O . Origin, biogenesis, and activity of plant microRNAs. Cell. 2009; 136(4):669-87. DOI: 10.1016/j.cell.2009.01.046. View

19.

Yamasaki H, Hayashi M, Fukazawa M, Kobayashi Y, Shikanai T . SQUAMOSA Promoter Binding Protein-Like7 Is a Central Regulator for Copper Homeostasis in Arabidopsis. Plant Cell. 2009; 21(1):347-61. PMC: 2648088. DOI: 10.1105/tpc.108.060137. View

20.

Howe G, Lightner J, Browse J, Ryan C . An octadecanoid pathway mutant (JL5) of tomato is compromised in signaling for defense against insect attack. Plant Cell. 1996; 8(11):2067-77. PMC: 161335. DOI: 10.1105/tpc.8.11.2067. View