Role of MiR-520b in Non-small Cell Lung Cancer

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2018 Nov 8

PMID 30402147

Citations 9

Authors

Linlin Zhang

Shuangquan Yu

Affiliations

Soon will be listed here.

Abstract

The aim of the present study was to investigate the expression of microRNA (miR)-520b in non-small cell lung cancer (NSCLC) and its biological functions. Reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-520b in 52 cases of NSCLC tissues, and its associations with tumor clinical staging and lymph node metastasis were analyzed. miR-520b mimics was transfected into A549 and Calu-3 cells. Cell proliferation, cell cycle, and cell invasion and migration abilities were assessed via cell counting kit-8 assay, flow cytometry and Transwell chamber assay, respectively. Western blot analysis was performed to detected protein expression levels, and dual luciferase reporter assay was used to detect the gene interaction. miR-520b expression was significantly downregulated in NSCLC. The expression of miR-520b in tumor tissues at N1 stage was lower than that at the N0 stage. miR-520b expression was negatively associated with clinical TNM staging. Furthermore, miR-520b mimic transfection inhibited the proliferation and invasion and metastasis abilities of A549 and Calu-3 cells. The expression of Rab22A was downregulated in the miR-520b mimics-transfected cells, whereas E-cadherin expression was increased, and vimentin expression was downregulated. Dual luciferase reporter assay demonstrated that miR-520b directly targeted the expression of Rab22A. Furthermore, Rab22A reversal downregulated the inhibitory effect of miR-520b. miR-520b expression was downregulated in NSCLC, which was negatively correlated with lymph node metastasis and TNM staging. miR-520b targeted on Rab22A to work as a tumor suppressor, inhibiting tumor proliferation and metastasis.

Citing Articles

Insights into the complex interactions between Rab22a and extracellular vesicles in cancers.

Huang S, Bao Y, Kong L, Gao S, Hua C Inflamm Res. 2023; 73(1):99-110.

PMID: 38066108 DOI: 10.1007/s00011-023-01821-0.

The Importance of the Immune System and Molecular Cell Signaling Pathways in the Pathogenesis and Progression of Lung Cancer.

Smok-Kalwat J, Mertowska P, Mertowski S, Smolak K, Kozinska A, Koszalka F Int J Mol Sci. 2023; 24(2).

PMID: 36675020 PMC: 9861992. DOI: 10.3390/ijms24021506.

Narrative review: molecular and genetic profiling of oligometastatic non-small cell lung cancer.

Rashdan S, Iyengar P, Minna J, Gerber D Transl Lung Cancer Res. 2021; 10(7):3351-3368.

PMID: 34430372 PMC: 8350108. DOI: 10.21037/tlcr-21-448.

Clinical Significance and Biological Function of miR-1274a in Non-small Cell Lung Cancer.

Zhu S, Wang X, Hu S, Fang Y, Guan B, Li J Mol Biotechnol. 2021; 64(1):9-16.

PMID: 34427871 DOI: 10.1007/s12033-021-00385-w.

MicroRNAs and Long Non-Coding RNAs as Potential Candidates to Target Specific Motifs of SARS-CoV-2.

Natarelli L, Parca L, Mazza T, Weber C, Virgili F, Fratantonio D Noncoding RNA. 2021; 7(1).

PMID: 33670580 PMC: 7931055. DOI: 10.3390/ncrna7010014.

References

Zhou Y, Wu B, Li J, Nan G, Jiang J, Chen Z . Rab22a enhances CD147 recycling and is required for lung cancer cell migration and invasion. Exp Cell Res. 2017; 357(1):9-16. DOI: 10.1016/j.yexcr.2017.04.020. View

Lu W, Zhang H, Niu Y, Wu Y, Sun W, Li H . Long non-coding RNA linc00673 regulated non-small cell lung cancer proliferation, migration, invasion and epithelial mesenchymal transition by sponging miR-150-5p. Mol Cancer. 2017; 16(1):118. PMC: 5504775. DOI: 10.1186/s12943-017-0685-9. View

Farooqi A, Shu C, Huang H, Wang H, Chang Y, Fayyaz S . TRAIL, Wnt, Sonic Hedgehog, TGFβ, and miRNA Signalings Are Potential Targets for Oral Cancer Therapy. Int J Mol Sci. 2017; 18(7). PMC: 5536013. DOI: 10.3390/ijms18071523. View

Proto C, Lo Russo G, Corrao G, Ganzinelli M, Facchinetti F, Minari R . Treatment in EGFR-mutated non-small cell lung cancer: how to block the receptor and overcome resistance mechanisms. Tumori. 2017; 103(4):325-337. DOI: 10.5301/tj.5000663. View

Yang X, Yin J, Xiang Q, Xie H, Yu J, Gan R . [MiR-503 sensitizes human hepatocellular carcinoma cells to cisplatin by targeting bcl-2]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2017; 42(6):605-610. DOI: 10.11817/j.issn.1672-7347.2017.06.001. View

Lu Y, Cheng A, Lee L, You G, Li Y, Chen H . MiR-520b as a novel molecular target for suppressing stemness phenotype of head-neck cancer by inhibiting CD44. Sci Rep. 2017; 7(1):2042. PMC: 5435724. DOI: 10.1038/s41598-017-02058-8. View

Yasukawa M, Kawaguchi T, Kawai N, Tojo T, Taniguchi S . [Prognostic Significance of Mode of Nodal Involvement in Pulmonary pN1 Squamous Cell Carcinoma]. Kyobu Geka. 2018; 71(3):163-168. View

Wang H, Tang M, Ou L, Hou M, Feng T, Huang Y . Biological analysis of cancer specific microRNAs on function modeling in osteosarcoma. Sci Rep. 2017; 7(1):5382. PMC: 5511279. DOI: 10.1038/s41598-017-05819-7. View

Li S, Zhang H, Ning T, Wang X, Liu R, Yang H . MiR-520b/e Regulates Proliferation and Migration by Simultaneously Targeting EGFR in Gastric Cancer. Cell Physiol Biochem. 2016; 40(6):1303-1315. DOI: 10.1159/000453183. View

10.

Zheng Z, Qu J, Yi H, Ye X, Huang W, Xiao T . MiR-125b regulates proliferation and apoptosis of nasopharyngeal carcinoma by targeting A20/NF-κB signaling pathway. Cell Death Dis. 2017; 8(6):e2855. PMC: 5520883. DOI: 10.1038/cddis.2017.211. View

11.

Moles R . MicroRNAs-based Therapy: A Novel and Promising Strategy for Cancer Treatment. Microrna. 2017; 6(2):102-109. DOI: 10.2174/2211536606666170710183039. View

12.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

13.

Wang T, Gilkes D, Takano N, Xiang L, Luo W, Bishop C . Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis. Proc Natl Acad Sci U S A. 2014; 111(31):E3234-42. PMC: 4128139. DOI: 10.1073/pnas.1410041111. View

14.

Yuwen D, Sheng B, Liu J, Wenyu W, Shu Y . MiR-146a-5p level in serum exosomes predicts therapeutic effect of cisplatin in non-small cell lung cancer. Eur Rev Med Pharmacol Sci. 2017; 21(11):2650-2658. View

15.

He J, Yu L, Wang C, Zhou X . MiR-1275 promotes non-small cell lung cancer cell proliferation and metastasis by regulating LZTS3 expression. Eur Rev Med Pharmacol Sci. 2018; 22(9):2680-2687. DOI: 10.26355/eurrev_201805_14964. View

16.

Ren D, Yang Q, Dai Y, Guo W, Du H, Song L . Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-κB signaling pathway. Mol Cancer. 2017; 16(1):117. PMC: 5504657. DOI: 10.1186/s12943-017-0688-6. View

17.

Xiong F, Liu K, Zhang F, Sha K, Wang X, Guo X . MiR-204 inhibits the proliferation and invasion of renal cell carcinoma by inhibiting RAB22A expression. Oncol Rep. 2016; 35(5):3000-8. DOI: 10.3892/or.2016.4624. View

18.

Muralimanoharan S, Guo C, Myatt L, Maloyan A . Sexual dimorphism in miR-210 expression and mitochondrial dysfunction in the placenta with maternal obesity. Int J Obes (Lond). 2015; 39(8):1274-81. PMC: 4526386. DOI: 10.1038/ijo.2015.45. View

19.

Botsa E, Thanou I, Papatheodoropoulou A, Thanos L . Thermal Ablation in the Management of Adrenal Metastasis Originating from Non-small Cell Lung Cancer: A 5-year Single-center Experience. Chin Med J (Engl). 2017; 130(17):2027-2032. PMC: 5586169. DOI: 10.4103/0366-6999.210496. View

20.

Ali A, Kim S, Kim M, Choi M, Kang S, Cho G . O-GlcNAcylation of NF-κB Promotes Lung Metastasis of Cervical Cancer Cells via Upregulation of CXCR4 Expression. Mol Cells. 2017; 40(7):476-484. PMC: 5547217. DOI: 10.14348/molcells.2017.2309. View