A Study on the Prevention and Treatment of Murine Calvarial Inflammatory Osteolysis Induced by Ultra-high-molecular-weight Polyethylene Particles with Neomangiferin

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2018 Nov 8

PMID 30402145

Citations 1

Authors

Hong-Tao Wang

Jia Li

Shi-Ting Ma

Wen-Yu Feng

Qi Wang

Hong-Yan Zhou

Jin-Min Zhao

Jun Yao

Affiliations

Soon will be listed here.

Abstract

The present study aimed to examine the influence of neomangiferin on murine calvarial inflammatory osteolysis induced by ultra-high-molecular-weight polyethylene (UHMWPE) particles. Eight-week-old male C57BL/J6 mice served as an inflammatory osteolysis model, in which UHMWPE particles were implanted into the calvarial subperiosteal space. The mice were randomly distributed into four groups and treated with different interventions; namely, a sham group [phosphate-buffered saline (PBS) injection and no UHMWPE particles], model group (PBS injection and implantation of UHMWPE particles), low-dose neomangiferin group (UHMWPE particles +2.5 mg/kg neomangiferin), and high-dose neomangiferin group (UHMWPE particles +5 mg/kg neomangiferin). Following 3 weeks of feeding according to the above regimens, celiac artery blood samples were collected for an enzyme-linked immunosorbent assay (ELISA) to determine the expression of receptor activator of nuclear factor-κB ligand (RANKL), osteoclast-related receptor (OSCAR), cross-linked C-telopeptide of type I collagen (CTX-1); osteoprotegerin (OPG), tumor necrosis factor (TNF)-α, and interleukin (IL)-1β. Subsequently, the mice were sacrificed by cervical dislocation following ether-inhalation anesthesia, and the skull was separated for osteolysis analysis by micro-computed tomography (micro-CT). Following hematoxylin and eosin staining, tartrate-resistant acid phosphatase (TRAP) staining was performed to observe the dissolution and destruction of the skull. The micro-CT results suggested that neomangiferin significantly inhibited the murine calvarial osteolysis and bone resorption induced by UHMWPE particles. In addition, the ELISA results showed that neomangiferin decreased the expression levels of osteoclast markers RANKL, OSCAR, CTX-1, TNF-α and IL-1β. By contrast, the levels of OPG increased with the neomangiferin dose. Histopathological examination revealed that the TRAP-positive cell count was significantly reduced in the neomangiferin-treated animals compared with that in the positive control group, and the degree of bone resorption was also markedly reduced. Neomangiferin was found to have significant anti-inflammatory effects and to inhibit osteoclastogenesis. Therefore, it has the potential to prevent the aseptic loosening of a prosthesis following artificial joint replacement.

Citing Articles

In Vitro Evaluation of the Interaction of Seven Biologically Active Components in with P-gp.

Dai J, He Y, Fang J, Wang H, Chao L, Zhao L Molecules. 2022; 27(23).

PMID: 36500651 PMC: 9740098. DOI: 10.3390/molecules27238556.

References

Lin T, Yao Z, Sato T, Keeney M, Li C, Pajarinen J . Suppression of wear-particle-induced pro-inflammatory cytokine and chemokine production in macrophages via NF-κB decoy oligodeoxynucleotide: a preliminary report. Acta Biomater. 2014; 10(8):3747-55. PMC: 4081023. DOI: 10.1016/j.actbio.2014.04.034. View

Liu G, Ma C, Wang P, Zhang P, Qu X, Liu S . Pilose antler peptide potentiates osteoblast differentiation and inhibits osteoclastogenesis via manipulating the NF-κB pathway. Biochem Biophys Res Commun. 2017; 491(2):388-395. DOI: 10.1016/j.bbrc.2017.07.091. View

Seo C, Ha H, Kim Y, Jungb J . HPLC-pDA simultaneous determination and protective effect of Anemarrhena asphodeloides against acute renal failure. Nat Prod Commun. 2014; 9(6):829-32. View

Boyle W, Simonet W, Lacey D . Osteoclast differentiation and activation. Nature. 2003; 423(6937):337-42. DOI: 10.1038/nature01658. View

Jamsen E, Kouri V, Olkkonen J, Cor A, Goodman S, Konttinen Y . Characterization of macrophage polarizing cytokines in the aseptic loosening of total hip replacements. J Orthop Res. 2014; 32(9):1241-6. DOI: 10.1002/jor.22658. View

Petit A, Mwale F, Antoniou J, Zukor D, Huk O . Effect of bisphosphonates on the stimulation of macrophages by alumina ceramic particles: a comparison with ultra-high-molecular-weight polyethylene. J Mater Sci Mater Med. 2006; 17(7):667-73. DOI: 10.1007/s10856-006-9230-x. View

Nie S, Xu J, Zhang C, Xu C, Liu M, Yu D . Salicortin inhibits osteoclast differentiation and bone resorption by down-regulating JNK and NF-κB/NFATc1 signaling pathways. Biochem Biophys Res Commun. 2016; 470(1):61-67. DOI: 10.1016/j.bbrc.2015.12.115. View

Song F, Wei C, Zhou L, Qin A, Yang M, Tickner J . Luteoloside prevents lipopolysaccharide-induced osteolysis and suppresses RANKL-induced osteoclastogenesis through attenuating RANKL signaling cascades. J Cell Physiol. 2017; 233(2):1723-1735. DOI: 10.1002/jcp.26084. View

He L, Li X, Zeng X, Duan H, Wang S, Lei L . Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation. Acta Pharmacol Sin. 2013; 35(2):203-10. PMC: 4651217. DOI: 10.1038/aps.2013.139. View

10.

Zhu X, Gao J, Ng P, Qin A, Steer J, Pavlos N . Alexidine Dihydrochloride Attenuates Osteoclast Formation and Bone Resorption and Protects Against LPS-Induced Osteolysis. J Bone Miner Res. 2015; 31(3):560-72. DOI: 10.1002/jbmr.2710. View

11.

Goodman S, Gibon E, Pajarinen J, Lin T, Keeney M, Ren P . Novel biological strategies for treatment of wear particle-induced periprosthetic osteolysis of orthopaedic implants for joint replacement. J R Soc Interface. 2014; 11(93):20130962. PMC: 3928932. DOI: 10.1098/rsif.2013.0962. View

12.

Lim S, Kang G, Jeong J, Choi H, Kim D . Neomangiferin modulates the Th17/Treg balance and ameliorates colitis in mice. Phytomedicine. 2016; 23(2):131-40. DOI: 10.1016/j.phymed.2016.01.002. View

13.

Chiu R, Ma T, Smith R, Goodman S . Polymethylmethacrylate particles inhibit osteoblastic differentiation of bone marrow osteoprogenitor cells. J Biomed Mater Res A. 2006; 77(4):850-6. DOI: 10.1002/jbm.a.30697. View

14.

Newman D, Cragg G . Natural Products as Sources of New Drugs from 1981 to 2014. J Nat Prod. 2016; 79(3):629-61. DOI: 10.1021/acs.jnatprod.5b01055. View

15.

Sabokbar A, Kudo O, Athanasou N . Two distinct cellular mechanisms of osteoclast formation and bone resorption in periprosthetic osteolysis. J Orthop Res. 2003; 21(1):73-80. DOI: 10.1016/S0736-0266(02)00106-7. View

16.

Zhou C, Zhou J, Han N, Liu Z, Xiao B, Yin J . Beneficial effects of neomangiferin on high fat diet-induced nonalcoholic fatty liver disease in rats. Int Immunopharmacol. 2015; 25(1):218-28. DOI: 10.1016/j.intimp.2015.01.027. View

17.

Zhai Z, Qu X, Li H, Yang K, Wan P, Tan L . The effect of metallic magnesium degradation products on osteoclast-induced osteolysis and attenuation of NF-κB and NFATc1 signaling. Biomaterials. 2014; 35(24):6299-310. DOI: 10.1016/j.biomaterials.2014.04.044. View

18.

Zheng M, Ge Y, Li H, Yan M, Zhou J, Zhang Y . Bergapten prevents lipopolysaccharide mediated osteoclast formation, bone resorption and osteoclast survival. Int Orthop. 2013; 38(3):627-34. PMC: 3936084. DOI: 10.1007/s00264-013-2184-y. View

19.

Goodman S, Gibon E, Yao Z . The basic science of periprosthetic osteolysis. Instr Course Lect. 2013; 62:201-6. PMC: 3766766. View

20.

Ingham E, Fisher J . The role of macrophages in osteolysis of total joint replacement. Biomaterials. 2004; 26(11):1271-86. DOI: 10.1016/j.biomaterials.2004.04.035. View