Genetic Resistance to Avian Leukosis Viruses Induced by CRISPR/Cas9 Editing of Specific Receptor Genes in Chicken Cells

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2018 Nov 8

PMID 30400152

Citations 24

Authors

Anna Koslova

Dana Kucerova

Marketa Reinisova

Josef Geryk

Pavel Trefil

Jiri Hejnar

Affiliations

Soon will be listed here.

Abstract

Avian leukosis viruses (ALVs), which are pathogens of concern in domestic poultry, utilize specific receptor proteins for cell entry that are both necessary and sufficient for host susceptibility to a given ALV subgroup. This unequivocal relationship offers receptors as suitable targets of selection and biotechnological manipulation with the aim of obtaining virus-resistant poultry. This approach is further supported by the existence of natural knock-outs of receptor genes that segregate in inbred lines of chickens. We used CRISPR/Cas9 genome editing tools to introduce frame-shifting indel mutations into , , and loci encoding receptors for the A, C, and J ALV subgroups, respectively. For all three loci, the homozygous frame-shifting indels generating premature stop codons induced phenotypes which were fully resistant to the virus of respective subgroup. In the locus, we also obtained in-frame deletions corroborating the importance of W38 and the four amino-acids preceding it. We demonstrate that CRISPR/Cas9-mediated knock-out or the fine editing of ALV receptor genes might be the first step in the development of virus-resistant chickens.

Citing Articles

Antiviral Effects of Avian Interferon-Stimulated Genes.

He X, Zhang S, Zou Z, Gao P, Yang L, Xiang B Animals (Basel). 2024; 14(21).

PMID: 39518785 PMC: 11545081. DOI: 10.3390/ani14213062.

Rapid adaptive evolution of avian leukosis virus subgroup J in response to biotechnologically induced host resistance.

Matouskova M, Plachy J, Kucerova D, Pecnova L, Reinisova M, Geryk J PLoS Pathog. 2024; 20(8):e1012468.

PMID: 39146367 PMC: 11349186. DOI: 10.1371/journal.ppat.1012468.

Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology.

Mishu M, Nath S, Sohidullah M, Hossain M J Adv Vet Anim Res. 2024; 11(2):483-493.

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Perspectives in Genome-Editing Techniques for Livestock.

Popova J, Bets V, Kozhevnikova E Animals (Basel). 2023; 13(16).

PMID: 37627370 PMC: 10452040. DOI: 10.3390/ani13162580.

Avian Leukosis: Will We Be Able to Get Rid of It?.

Fandino S, Gomez-Lucia E, Benitez L, Domenech A Animals (Basel). 2023; 13(14).

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