A Lateral Flow Assay for Simultaneous Detection of Deoxynivalenol, Fumonisin B and Aflatoxin B

Deoxynivalenol (DON), Fumonisin B (FB) and Aflatoxin B (AFB) have strong toxicity to humans and exist widely in grain and food. It is necessary to screen these mycotoxins to guarantee food safety. In order to develop a rapid, simple, low-cost and simultaneous-detection method, composites of antibody-nano-Au-particles, DON-BSA, FB-BSA, and AFB-BSA were prepared to establish a lateral flow assay based on competitive inhibition. The results suggested that the visual detection limits for DON, FB and AFB were 10, 30, and 10 ng mL, respectively. On the other hand, it had no reactivity to T-2 toxin, zearalenone, ochratoxin A and nivalenol, which demonstrates good specificity. Besides, the strip had good repeatability and stability. Therefore, DON, FB and AFB would be screened simultaneously by lateral flow assay for food safety.