Baicalein Protects Renal Tubular Epithelial Cells Againsthypoxia-reoxygenation Injury

Overview

Journal Ren Fail

Publisher Informa Healthcare

Date 2018 Nov 3

PMID 30384801

Citations 5

Authors

Chun Chen

Chudan Cai

Hanfei Lin

Weidai Zhang

Yanqiang Peng

Kefei Wu

Affiliations

Soon will be listed here.

Abstract

Background: To investigate the protective effects and mechanism of baicalein (BAI), a naturally occurring flavonoid, against hypoxia-reoxygenation (HR) injury in renal tubular epithelial cells (HK-2).

Methods: Cultured human renal proximal tubular cell line HK-2 was exposed to 24 h of hypoxia (5% CO, 1% O, and 94% N), followed by 12 h of reoxygenation (5% CO, 21% O, and 74% N). HK-2 cells were divided into three groups: control, HR, and HR-BAI (0.3 µg/ml). Reactive oxygen species (ROS) were measured and cell apoptosis was analyzed by flow cytometry and morphology. ELISAs were performed to determine the levels of IL-1, intercellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1). IL-1β, ICAM-1, and MCP-1 mRNA levels were determined by real-time quantitative PCR.

Results: HK-2 cells that underwent HR exhibited increases in IL-1β expression by 0.94%, ROS by 0.59%, ICAM-1 expression by 0.8%, and MCP-1 expression by 1.2%. Moreover, HK-2 cell apoptosis was increased after HR (p < .05). Compared with the HR group, BAI treatment reduced the elevation of oxidative stress (ROS) by 0.76%, as well as HR-mediated induction of IL-1β and apoptosis of HK2 cells. Protein and mRNA levels of ICAM-1 and MCP-1 were also reduced.

Conclusions: BAI protects renal tubular epithelial cells from HR injury by reducing inflammatory cytokine expression and oxidative stress.

Citing Articles

Glutathione metabolism rewiring protects renal tubule cells against cisplatin-induced apoptosis and ferroptosis.

Dong X, Chu L, Cao X, Xiong Q, Mao Y, Chen C Redox Rep. 2023; 28(1):2152607.

PMID: 36692085 PMC: 9879199. DOI: 10.1080/13510002.2022.2152607.

Baicalein and Αlpha-Tocopherol Inhibit Toll-like Receptor Pathways in Cisplatin-Induced Nephrotoxicity.

Awadalla A, Mahdi M, Zahran M, Abdelbaset-Ismail A, El-Dosoky M, Negm A Molecules. 2022; 27(7).

PMID: 35408581 PMC: 9000769. DOI: 10.3390/molecules27072179.

Nephroprotective Role of Chrysophanol in Hypoxia/Reoxygenation-Induced Renal Cell Damage via Apoptosis, ER Stress, and Ferroptosis.

Lin C, Tseng H, Hsieh P, Chiu V, Lin T, Lan C Biomedicines. 2021; 9(9).

PMID: 34572468 PMC: 8467645. DOI: 10.3390/biomedicines9091283.

Inhibition of Oxidative Stress and ALOX12 and NF-κB Pathways Contribute to the Protective Effect of Baicalein on Carbon Tetrachloride-Induced Acute Liver Injury.

Dai C, Li H, Wang Y, Tang S, Velkov T, Shen J Antioxidants (Basel). 2021; 10(6).

PMID: 34207230 PMC: 8235740. DOI: 10.3390/antiox10060976.

Baicalein Neutralizes Hypercholesterolemia-Induced Aggravation of Oxidative Injury in Rats.

AlSaad A, Mohany M, Almalki M, Almutham I, Alahmari A, Alsulaiman M Int J Med Sci. 2020; 17(9):1156-1166.

PMID: 32547311 PMC: 7294921. DOI: 10.7150/ijms.46108.

References

Hao X, Yao A, Gong J, Zhu W, Li N, Li J . Berberine ameliorates pro-inflammatory cytokine-induced endoplasmic reticulum stress in human intestinal epithelial cells in vitro. Inflammation. 2011; 35(3):841-9. DOI: 10.1007/s10753-011-9385-6. View

Hoste E, Bagshaw S, Bellomo R, Cely C, Colman R, Cruz D . Epidemiology of acute kidney injury in critically ill patients: the multinational AKI-EPI study. Intensive Care Med. 2015; 41(8):1411-23. DOI: 10.1007/s00134-015-3934-7. View

Tsutsui H, Shimokawa T, Miura T, Takama M, Nishinaka T, Terada T . Effect of monoamine oxidase inhibitors on ischaemia/reperfusion-induced acute kidney injury in rats. Eur J Pharmacol. 2017; 818:38-42. DOI: 10.1016/j.ejphar.2017.10.021. View

Schiffl H . Prevention of severe acute kidney injury by implementation of care bundles: Some progress but still a lot of work ahead. Saudi J Kidney Dis Transpl. 2018; 29(3):513-517. DOI: 10.4103/1319-2442.235195. View

Tumlin J, Stacul F, Adam A, Becker C, Davidson C, Lameire N . Pathophysiology of contrast-induced nephropathy. Am J Cardiol. 2006; 98(6A):14K-20K. DOI: 10.1016/j.amjcard.2006.01.020. View

Baradaran A, Nasri H, Rafieian-Kopaei M . Protection of renal tubular cells by antioxidants: current knowledge and new trends. Cell J. 2015; 16(4):568-71. PMC: 4297497. DOI: 10.22074/cellj.2015.503. View

van Rijt W, Nieuwenhuijs-Moeke G, Goor H, Ottens P, Ploeg R, Leuvenink H . Renoprotective capacities of non-erythropoietic EPO derivative, ARA290, following renal ischemia/reperfusion injury. J Transl Med. 2013; 11:286. PMC: 3842642. DOI: 10.1186/1479-5876-11-286. View

Song L, Yang H, Wang H, Tian C, Liu Y, Zeng X . Inhibition of 12/15 lipoxygenase by baicalein reduces myocardial ischemia/reperfusion injury via modulation of multiple signaling pathways. Apoptosis. 2013; 19(4):567-80. DOI: 10.1007/s10495-013-0946-z. View

Park J . Postoperative acute kidney injury. Korean J Anesthesiol. 2017; 70(3):258-266. PMC: 5453887. DOI: 10.4097/kjae.2017.70.3.258. View

10.

Sun Y, Xun L, Jin G, Shi L . Salidroside protects renal tubular epithelial cells from hypoxia/reoxygenation injury in vitro. J Pharmacol Sci. 2018; 137(2):170-176. DOI: 10.1016/j.jphs.2018.05.011. View

11.

Ozturk H, Cetinkaya A, Duzcu S, Tekce B, Ozturk H . Carvacrol attenuates histopathogic and functional impairments induced by bilateral renal ischemia/reperfusion in rats. Biomed Pharmacother. 2018; 98:656-661. DOI: 10.1016/j.biopha.2017.12.060. View

12.

Gao D, Tawa R, Masaki H, Okano Y, Sakurai H . Protective effects of baicalein against cell damage by reactive oxygen species. Chem Pharm Bull (Tokyo). 1998; 46(9):1383-7. DOI: 10.1248/cpb.46.1383. View

13.

Schrier R, Wang W, Poole B, Mitra A . Acute renal failure: definitions, diagnosis, pathogenesis, and therapy. J Clin Invest. 2004; 114(1):5-14. PMC: 437979. DOI: 10.1172/JCI22353. View

14.

Gameiro J, Agapito Fonseca J, Neves M, Jorge S, Antonio Lopes J . Acute kidney injury in major abdominal surgery: incidence, risk factors, pathogenesis and outcomes. Ann Intensive Care. 2018; 8(1):22. PMC: 5807256. DOI: 10.1186/s13613-018-0369-7. View

15.

Chien C, Lee P, Chen C, Ma M, Lai M, Hsu S . De novo demonstration and co-localization of free-radical production and apoptosis formation in rat kidney subjected to ischemia/reperfusion. J Am Soc Nephrol. 2001; 12(5):973-982. DOI: 10.1681/ASN.V125973. View

16.

Susin S, Daugas E, Ravagnan L, Samejima K, Zamzami N, Loeffler M . Two distinct pathways leading to nuclear apoptosis. J Exp Med. 2000; 192(4):571-80. PMC: 2193229. DOI: 10.1084/jem.192.4.571. View

17.

Ge Y, Zhang Y, Li R, Chen W, Li Y, Chen G . Berberine regulated Gck, G6pc, Pck1 and Srebp-1c expression and activated AMP-activated protein kinase in primary rat hepatocytes. Int J Biol Sci. 2011; 7(5):673-84. PMC: 3107476. DOI: 10.7150/ijbs.7.673. View

18.

Brezis M, Rosen S . Hypoxia of the renal medulla--its implications for disease. N Engl J Med. 1995; 332(10):647-55. DOI: 10.1056/NEJM199503093321006. View

19.

Bruno M, Piozzi F, Rosselli S . Natural and hemisynthetic neoclerodane diterpenoids from scutellaria and their antifeedant activity. Nat Prod Rep. 2002; 19(3):357-78. DOI: 10.1039/b111150g. View

20.

Jung E, Lee C . Baicalein attenuates proteasome inhibition-induced apoptosis by suppressing the activation of the mitochondrial pathway and the caspase-8- and Bid-dependent pathways. Eur J Pharmacol. 2014; 730:116-24. DOI: 10.1016/j.ejphar.2014.02.039. View