Developments with 3D Bioprinting for Novel Drug Discovery

Overview

Journal Expert Opin Drug Discov

Specialties Chemistry
Pharmacology

Date 2018 Nov 3

PMID 30384781

Citations 24

Authors

Aishwarya Satpathy

Pallab Datta

Yang Wu

Bugra Ayan

Ertugrul Bayram

Ibrahim T Ozbolat

Affiliations

Soon will be listed here.

Abstract

: Although there have been significant contributions from the pharmaceutical industry to clinical practice, several diseases remain unconquered, with the discovery of new drugs remaining a paramount objective. The actual process of drug discovery involves many steps including pre-clinical and clinical testing, which are highly time- and resource-consuming, driving researchers to improve the process efficiency. The shift of modelling technology from two-dimensions (2D) to three-dimensions (3D) is one of such advancements. 3D Models allow for close mimicry of cellular interactions and tissue microenvironments thereby improving the accuracy of results. The advent of bioprinting for fabrication of tissues has shown potential to improve 3D culture models. : The present review provides a comprehensive update on a wide range of bioprinted tissue models and appraise them for their potential use in drug discovery research. : Efficiency, reproducibility, and standardization are some impediments of the bioprinted models. Vascularization of the constructs has to be addressed in the near future. While much progress has already been made with several seminal works, the next milestone will be the commercialization of these models after due regulatory approval.

Citing Articles

3D bioprinting for the construction of drug testing models-development strategies and regulatory concerns.

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High-throughput bioprinting of spheroids for scalable tissue fabrication.

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Embedded 3D bioprinting - An emerging strategy to fabricate biomimetic & large vascularized tissue constructs.

Budharaju H, Sundaramurthi D, Sethuraman S Bioact Mater. 2023; 32:356-384.

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Three Dimensional Bioprinting for Hepatic Tissue Engineering: From In Vitro Models to Clinical Applications.

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References

Underhill G, Khetani S . Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies. Cell Mol Gastroenterol Hepatol. 2018; 5(3):426-439.e1. PMC: 5904032. DOI: 10.1016/j.jcmgh.2017.11.012. View

Langhans S . Three-Dimensional Cell Culture Models in Drug Discovery and Drug Repositioning. Front Pharmacol. 2018; 9:6. PMC: 5787088. DOI: 10.3389/fphar.2018.00006. View

Bonnier F, Keating M, Wrobel T, Majzner K, Baranska M, Garcia-Munoz A . Cell viability assessment using the Alamar blue assay: a comparison of 2D and 3D cell culture models. Toxicol In Vitro. 2014; 29(1):124-31. DOI: 10.1016/j.tiv.2014.09.014. View

Fang Y, Eglen R . Three-Dimensional Cell Cultures in Drug Discovery and Development. SLAS Discov. 2017; 22(5):456-472. PMC: 5448717. DOI: 10.1177/1087057117696795. View

Haisler W, Timm D, Gage J, Tseng H, Killian T, Souza G . Three-dimensional cell culturing by magnetic levitation. Nat Protoc. 2013; 8(10):1940-9. DOI: 10.1038/nprot.2013.125. View

Koch L, Deiwick A, Schlie S, Michael S, Gruene M, Coger V . Skin tissue generation by laser cell printing. Biotechnol Bioeng. 2012; 109(7):1855-63. DOI: 10.1002/bit.24455. View

Ozbolat I, Peng W, Ozbolat V . Application areas of 3D bioprinting. Drug Discov Today. 2016; 21(8):1257-71. DOI: 10.1016/j.drudis.2016.04.006. View

Gintant G, George C . Introduction to biological complexity as a missing link in drug discovery. Expert Opin Drug Discov. 2018; 13(8):753-763. DOI: 10.1080/17460441.2018.1480608. View

Wang Z, Lee S, Cheng H, Yoo J, Atala A . 3D bioprinted functional and contractile cardiac tissue constructs. Acta Biomater. 2018; 70:48-56. PMC: 6022829. DOI: 10.1016/j.actbio.2018.02.007. View

10.

Reichl S, Bednarz J, Muller-Goymann C . Human corneal equivalent as cell culture model for in vitro drug permeation studies. Br J Ophthalmol. 2004; 88(4):560-5. PMC: 1772077. DOI: 10.1136/bjo.2003.028225. View

11.

Madden L, Nguyen T, Garcia-Mojica S, Shah V, Le A, Peier A . Bioprinted 3D Primary Human Intestinal Tissues Model Aspects of Native Physiology and ADME/Tox Functions. iScience. 2018; 2:156-167. PMC: 6135981. DOI: 10.1016/j.isci.2018.03.015. View

12.

Cui X, Gao G, Qiu Y . Accelerated myotube formation using bioprinting technology for biosensor applications. Biotechnol Lett. 2012; 35(3):315-21. DOI: 10.1007/s10529-012-1087-0. View

13.

Cui X, Dean D, Ruggeri Z, Boland T . Cell damage evaluation of thermal inkjet printed Chinese hamster ovary cells. Biotechnol Bioeng. 2010; 106(6):963-9. DOI: 10.1002/bit.22762. View

14.

Baker B, Chen C . Deconstructing the third dimension: how 3D culture microenvironments alter cellular cues. J Cell Sci. 2012; 125(Pt 13):3015-24. PMC: 3434846. DOI: 10.1242/jcs.079509. View

15.

Pickl M, Ries C . Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab. Oncogene. 2008; 28(3):461-8. DOI: 10.1038/onc.2008.394. View

16.

Datta P, Barui A, Wu Y, Ozbolat V, Moncal K, Ozbolat I . Essential steps in bioprinting: From pre- to post-bioprinting. Biotechnol Adv. 2018; 36(5):1481-1504. DOI: 10.1016/j.biotechadv.2018.06.003. View

17.

Rodriguez-Devora J, Zhang B, Reyna D, Shi Z, Xu T . High throughput miniature drug-screening platform using bioprinting technology. Biofabrication. 2012; 4(3):035001. DOI: 10.1088/1758-5082/4/3/035001. View

18.

Snyder J, Hamid Q, Wang C, Chang R, Emami K, Wu H . Bioprinting cell-laden matrigel for radioprotection study of liver by pro-drug conversion in a dual-tissue microfluidic chip. Biofabrication. 2011; 3(3):034112. DOI: 10.1088/1758-5082/3/3/034112. View

19.

Nguyen D, Funk J, Robbins J, Crogan-Grundy C, Presnell S, Singer T . Bioprinted 3D Primary Liver Tissues Allow Assessment of Organ-Level Response to Clinical Drug Induced Toxicity In Vitro. PLoS One. 2016; 11(7):e0158674. PMC: 4936711. DOI: 10.1371/journal.pone.0158674. View

20.

Isaacson A, Swioklo S, Connon C . 3D bioprinting of a corneal stroma equivalent. Exp Eye Res. 2018; 173:188-193. PMC: 6083436. DOI: 10.1016/j.exer.2018.05.010. View