Microfluidics-enabled Rapid Manufacturing of Hierarchical Silica-magnetic Microflower Toward Enhanced Circulating Tumor Cell Screening

Overview

Journal Biomater Sci

Publisher Royal Society of Chemistry

Specialties Biomedical Engineering
Pharmacology

Date 2018 Oct 31

PMID 30375583

Citations 10

Authors

Nanjing Hao

Yuan Nie

Amogha Tadimety

Ting Shen

John X J Zhang

Affiliations

Soon will be listed here.

Abstract

The emergence of microfluidic techniques provides new opportunities for chemical synthesis and biomedical applications. Herein, we first develop a microfluidics-based flow and sustainable strategy to synthesize hierarchical silica-magnetic microflower with unique multilayered structure for the efficient capture of circulating tumor cells through our engineered microfluidic screening chip. The production of microflower materials can be realized within 94 milliseconds and a yield of nearly 5 grams per hour can be achieved. The enhanced bioaccessibility of such a multilayered microflower towards cancer cells (MCF-7 and MDA-MB-231) is demonstrated, and the cancer cell capture efficiency of this hierarchical immunomagnetic system in clinical blood samples is significantly increased compared with a standard CellSearch™ assay. These findings bring new insights for engineering functional micro-/nanomaterials in liquid biopsy.

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References

Hoshino K, Chen P, Huang Y, Zhang X . Computational analysis of microfluidic immunomagnetic rare cell separation from a particulate blood flow. Anal Chem. 2012; 84(10):4292-9. PMC: 3359653. DOI: 10.1021/ac2032386. View

Hao N, Nie Y, Tadimety A, Closson A, Zhang J . Microfluidics-mediated self-template synthesis of anisotropic hollow ellipsoidal mesoporous silica nanomaterials. Mater Res Lett. 2019; 5(8):584-590. PMC: 6405212. DOI: 10.1080/21663831.2017.1376720. View

Park J, Lee J, Lee J, Jeong H, Oh J, Kim Y . Highly efficient assay of circulating tumor cells by selective sedimentation with a density gradient medium and microfiltration from whole blood. Anal Chem. 2012; 84(17):7400-7. DOI: 10.1021/ac3011704. View

Valencia P, Farokhzad O, Karnik R, Langer R . Microfluidic technologies for accelerating the clinical translation of nanoparticles. Nat Nanotechnol. 2012; 7(10):623-9. PMC: 3654404. DOI: 10.1038/nnano.2012.168. View

Augustsson P, Magnusson C, Nordin M, Lilja H, Laurell T . Microfluidic, label-free enrichment of prostate cancer cells in blood based on acoustophoresis. Anal Chem. 2012; 84(18):7954-62. PMC: 3445767. DOI: 10.1021/ac301723s. View

Huang Y, Chen P, Wu C, Hoshino K, Sokolov K, Lane N . Screening and Molecular Analysis of Single Circulating Tumor Cells Using Micromagnet Array. Sci Rep. 2015; 5:16047. PMC: 4633592. DOI: 10.1038/srep16047. View

Lu Y, Liang H, Yu T, Xie J, Chen S, Dong H . Isolation and characterization of living circulating tumor cells in patients by immunomagnetic negative enrichment coupled with flow cytometry. Cancer. 2015; 121(17):3036-45. DOI: 10.1002/cncr.29444. View

Hoshino K, Huang Y, Lane N, Huebschman M, Uhr J, Frenkel E . Microchip-based immunomagnetic detection of circulating tumor cells. Lab Chip. 2011; 11(20):3449-57. PMC: 3379551. DOI: 10.1039/c1lc20270g. View

Liu Z, Huang F, Du J, Shu W, Feng H, Xu X . Rapid isolation of cancer cells using microfluidic deterministic lateral displacement structure. Biomicrofluidics. 2014; 7(1):11801. PMC: 3555967. DOI: 10.1063/1.4774308. View

10.

Chen P, Huang Y, Hoshino K, Zhang J . Microscale magnetic field modulation for enhanced capture and distribution of rare circulating tumor cells. Sci Rep. 2015; 5:8745. PMC: 4348664. DOI: 10.1038/srep08745. View

11.

Han K, Li C, Seong G . Microfluidic chips for immunoassays. Annu Rev Anal Chem (Palo Alto Calif). 2013; 6:119-41. DOI: 10.1146/annurev-anchem-062012-092616. View

12.

Gratton S, Ropp P, Pohlhaus P, Luft J, Madden V, Napier M . The effect of particle design on cellular internalization pathways. Proc Natl Acad Sci U S A. 2008; 105(33):11613-8. PMC: 2575324. DOI: 10.1073/pnas.0801763105. View

13.

Blanco E, Shen H, Ferrari M . Principles of nanoparticle design for overcoming biological barriers to drug delivery. Nat Biotechnol. 2015; 33(9):941-51. PMC: 4978509. DOI: 10.1038/nbt.3330. View

14.

Elvira K, Casadevall I Solvas X, Wootton R, deMello A . The past, present and potential for microfluidic reactor technology in chemical synthesis. Nat Chem. 2013; 5(11):905-15. DOI: 10.1038/nchem.1753. View

15.

Zborowski M, Chalmers J . Rare cell separation and analysis by magnetic sorting. Anal Chem. 2011; 83(21):8050-6. PMC: 3205221. DOI: 10.1021/ac200550d. View

16.

Alix-Panabieres C, Pantel K . Challenges in circulating tumour cell research. Nat Rev Cancer. 2014; 14(9):623-31. DOI: 10.1038/nrc3820. View

17.

Hyun K, Lee T, Jung H . Negative enrichment of circulating tumor cells using a geometrically activated surface interaction chip. Anal Chem. 2013; 85(9):4439-45. DOI: 10.1021/ac3037766. View

18.

Nie Y, Hao N, Zhang J . Ultrafast Synthesis of Multifunctional Submicrometer Hollow Silica Spheres in Microfluidic Spiral Channels. Sci Rep. 2017; 7(1):12616. PMC: 5626769. DOI: 10.1038/s41598-017-12856-9. View

19.

Geng Y, Dalhaimer P, Cai S, Tsai R, Tewari M, Minko T . Shape effects of filaments versus spherical particles in flow and drug delivery. Nat Nanotechnol. 2008; 2(4):249-55. PMC: 2740330. DOI: 10.1038/nnano.2007.70. View

20.

Tadimety A, Closson A, Li C, Yi S, Shen T, Zhang J . Advances in liquid biopsy on-chip for cancer management: Technologies, biomarkers, and clinical analysis. Crit Rev Clin Lab Sci. 2018; 55(3):140-162. PMC: 6101655. DOI: 10.1080/10408363.2018.1425976. View