In Vivo Extracellular PH Mapping of Tumors Using Electron Paramagnetic Resonance

Overview

Journal Anal Chem

Specialty Chemistry

Date 2018 Oct 30

PMID 30372035

Citations 17

Authors

Denis A Komarov

Yuki Ichikawa

Kumiko Yamamoto

Neil J Stewart

Shingo Matsumoto

Hironobu Yasui

Igor A Kirilyuk

Valery V Khramtsov

Osamu Inanami

Hiroshi Hirata

Affiliations

Soon will be listed here.

Abstract

An electron paramagnetic resonance (EPR)-based method for noninvasive three-dimensional extracellular pH mapping was developed using a pH-sensitive nitroxyl radical as an exogenous paramagnetic probe. Fast projection scanning with a constant magnetic field sweep enabled the acquisition of four-dimensional (3D spatial +1D spectral) EPR images within 7.5 min. Three-dimensional maps of pH were reconstructed by processing the pH-dependent spectral information on the images. To demonstrate the proposed method of pH mapping, the progress of extracellular acidosis in tumor-bearing mouse legs was studied. Furthermore, extracellular pH mapping was used to visualize the spatial distribution of acidification in different tumor xenograft mouse models of human-derived pancreatic ductal adenocarcinoma cells. The proposed EPR-based pH mapping method enabled quantitative visualization of regional changes in extracellular pH associated with altered tumor metabolism.

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