The Constitutive, Glucose-repression-insensitive Mutation of the Yeast MAL4 Locus is an Alteration of the MAL43 Gene

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1987 May 1

PMID 3036644

Citations 17

Authors

M J Charron

C A Michels

Affiliations

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Abstract

Mutations resulting in constitutive production of maltase have been identified at each of the five MAL loci of Saccharomyces yeasts. Here we examine a dominant constitutive, glucose-repression-insensitive allele of the MAL4 locus (MAL4-C). Our results demonstrate that MAL4-C is an alteration in the MAL43 gene, which encodes the positive regulator of the MAL structural genes, and that its product is trans-acting. The MAL43 gene from the MAL4-C strain was cloned and integrated into a series of nonfermenting strains lacking a functional regulatory gene but carrying copies of the maltose permease and maltase structural genes. Expression of the maltase structural gene was both constitutive and insensitive to glucose repression in these transformants. The MAL4-C allele also results in constitutive expression of the unlinked MAL12 gene (encoding maltase) in this strain. In addition, the cloned MAL43 gene was shown to be dominant to the wild-type MAL63 gene. We also show that most of the glucose repression insensitivity of strains carrying the MAL4-C allele results from alteration of MAL43.

Citing Articles

Hsp90 cochaperone Aha1 is a negative regulator of the Saccharomyces MAL activator and acts early in the chaperone activation pathway.

Ran F, Gadura N, Michels C J Biol Chem. 2010; 285(18):13850-62.

PMID: 20177068 PMC: 2859548. DOI: 10.1074/jbc.M109.040600.

Hsp90/Hsp70 chaperone machine regulation of the Saccharomyces MAL-activator as determined in vivo using noninducible and constitutive mutant alleles.

Ran F, Bali M, Michels C Genetics. 2008; 179(1):331-43.

PMID: 18458105 PMC: 2390613. DOI: 10.1534/genetics.107.084921.

Genetic Analysis of Haploids from Industrial Strains of Baker's Yeast.

Oda Y, Ouchi K Appl Environ Microbiol. 1989; 55(7):1742-7.

PMID: 16347967 PMC: 202944. DOI: 10.1128/aem.55.7.1742-1747.1989.

Analysis of the mechanism by which glucose inhibits maltose induction of MAL gene expression in Saccharomyces.

Hu Z, Yue Y, Jiang H, Zhang B, SHERWOOD P, Michels C Genetics. 2000; 154(1):121-32.

PMID: 10628974 PMC: 1460925. DOI: 10.1093/genetics/154.1.121.

Genetic evidence that high noninduced maltase and maltose permease activities, governed by MALx3-encoded transcriptional regulators, determine efficiency of gas production by baker's yeast in unsugared dough.

Higgins V, Braidwood M, Bell P, Bissinger P, Dawes I, Attfield P Appl Environ Microbiol. 1999; 65(2):680-5.

PMID: 9925600 PMC: 91079. DOI: 10.1128/AEM.65.2.680-685.1999.

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